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@misc{483353,
author = {Havliš, Jan and Revilla Vázquez, Alma Luísa and Kozák, Libor and Havel, Josef},
address = {23rd HPLC, Granada, Spain},
keywords = {DNA methylation; HPLC; MALDI-TOF MS; ED-ANN},
language = {eng},
location = {23rd HPLC, Granada, Spain},
publisher = {n / a},
title = {Determination of purins, pyrimidines and their nucleosides in urine by HPLC and MALDI-TOF mass spectrometry},
year = {1999}
}
TY - GEN
ID - 483353
AU - Havliš, Jan - Revilla Vázquez, Alma Luísa - Kozák, Libor - Havel, Josef
PY - 1999
TI - Determination of purins, pyrimidines and their nucleosides in urine by HPLC and MALDI-TOF mass spectrometry
VL - n / a
PB - n / a
CY - 23rd HPLC, Granada, Spain
KW - DNA methylation
KW - HPLC
KW - MALDI-TOF MS
KW - ED-ANN
N2 - Experimental design combined with artificial neural networks enables faster optimization of the separation conditions than classical single variable approach. HPLC can be successfully used for the determination of the state of 2-deoxycytidine 5-monophosphate methylation in DNA. Utilization of ED-ANN within HPLC has greatly enhanced the method of quantitative determination of 5-methyl-2'-deoxycytidine 5'-monophosphate and 2'-deoxycytidine 5'-monophosphate in the cell homogenate. Use of this approach has allowed us to optimize separation of several types of mixtures containing purine and pyrimidine bases, their nucleosides and nucleotides.
ER -
HAVLIŠ, Jan, Alma Luísa REVILLA VÁZQUEZ, Libor KOZÁK a Josef HAVEL. \textit{Determination of purins, pyrimidines and their nucleosides in urine by HPLC and MALDI-TOF mass spectrometry}. 23rd HPLC, Granada, Spain: n / a, 1999. n / a.
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