| WESIERSKA-GADEK, Jozefa, Daniela SCHLOFFER, Vladimír KOTALA a Marcel HORKÝ. Escape of p53 protein from E6-mediated degradation in HeLa cells after cisplatin therapy. International Journal of Cancer. USA: Wiley InterScience, 2002, roč. 101, č. 2, s. 128-135. ISSN 0020-7136. |
Další formáty:
BibTeX
LaTeX
RIS
@article{483355,
author = {WesierskaandGadek, Jozefa and Schloffer, Daniela and Kotala, Vladimír and Horký, Marcel},
article_location = {USA},
article_number = {2},
keywords = {apoptosis; nucleolar segregation; E6; cisplatin; PARP-1},
language = {eng},
issn = {0020-7136},
journal = {International Journal of Cancer},
title = {Escape of p53 protein from E6-mediated degradation in HeLa cells after cisplatin therapy},
volume = {101},
year = {2002}
}
TY - JOUR
ID - 483355
AU - Wesierska-Gadek, Jozefa - Schloffer, Daniela - Kotala, Vladimír - Horký, Marcel
PY - 2002
TI - Escape of p53 protein from E6-mediated degradation in HeLa cells after cisplatin therapy
JF - International Journal of Cancer
VL - 101
IS - 2
SP - 128
EP - 128
PB - Wiley InterScience
SN - 00207136
KW - apoptosis
KW - nucleolar segregation
KW - E6
KW - cisplatin
KW - PARP-1
N2 - We previously reported that therapy of human cervical carcinoma HeLa cells with CP induced segregation of nucleoli and changes of nuclei characteristic of apoptosis. We raised the question of whether p53 can be reactivated by chemotherapy in HeLa cells despite the presence of HPV-encoded E6 activity. Since the reduced stability of wild-type p53 protein in HeLa cells is a consequence of its enhanced ubiquitination by virally encoded E6 protein, resulting in its accelerated degradation, we checked the cellular level of E6 during CP therapy. Six hours after application of CP, E6 protein expression was markedly reduced. This coincided with the increase of cellular p53 and preceded the nucleolar accumulation of p53 protein, indicating that repression of virally coded E6 protein by CP contributes to the restoration of p53 expression.
ER -
WESIERSKA-GADEK, Jozefa, Daniela SCHLOFFER, Vladimír KOTALA a Marcel HORKÝ. Escape of p53 protein from E6-mediated degradation in HeLa cells after cisplatin therapy. \textit{International Journal of Cancer}. USA: Wiley InterScience, 2002, roč.~101, č.~2, s.~128-135. ISSN~0020-7136.
|
