Induction of apoptosis in human cervix carcinoma cells during
therapy by cisplatin

J 2002

Induction of apoptosis in human cervix carcinoma cells during therapy by cisplatin

SCHLOFFER, Daniela, Marcel HORKÝ, Vladimír KOTALA a Jozefa WESIERSKA-GADEK

Základní údaje

Originální název

Induction of apoptosis in human cervix carcinoma cells during therapy by cisplatin

Autoři

SCHLOFFER, Daniela (40 Rakousko), Marcel HORKÝ (203 Česká republika), Vladimír KOTALA (203 Česká republika) a Jozefa WESIERSKA-GADEK (40 Rakousko, garant)

Vydání

Cancer Detection and Prevention, USA, x, 2002, 0361-090X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.289

Kód RIV

RIV/00216224:14330/02:00007252

Organizační jednotka

Fakulta informatiky

UT WoS

000187247300010

Klíčová slova anglicky

activated caspases; cytochrome C release; DNA replication; mitosis; nucleolar segregation; PARP-1 degradation

Štítky

activated caspases, cytochrome C release, DNA replication, mitosis, nucleolar segregation, PARP-1 degradation

Změněno: 3. 6. 2003 10:56, Mgr. Vladimír Kotala, Ph.D.

Anotace

V originále

The aim of the therapy of human malignancies is the inhibition of cell proliferation and/or induction of apoptosis. We studied the kinetics of the morphological and biochemical changes in HeLa cells during chemotherapy by cisplatin. Apoptosis was evaluated by scoring of cells exhibiting changes characteristic for early and late stages of apoptosis as determined by Hoehst 33258 staining and by examination of positive reaction for activated caspase-3. Expression and intracellular localization of distinct proteins eas analyzed by immunoblotting of subcellular fractions and segregation of nucleoli by immunocytochemistry.Chromatin fragmentation characteristic for apoptosis was observed in single cells after 3h cisplatin. A strong cytoplasmic accumulation of cytochrome C detected by immunoblotting 6h post-treatment was accompanied by an activation of caspase-9. Neither inhibition of cell division nor blocking of DNA replication preceded the onset of apoptosis, Our results show that after short treatment by cisplatin cell proliferation and apoptosis concomitantly occurred

Návaznosti

MSM 141100002, záměr

Název: Molekulární patofyziologie multigenních chorob

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární patofyziologie multigenních chorob

Citovat

SCHLOFFER, Daniela, Marcel HORKÝ, Vladimír KOTALA a Jozefa WESIERSKA-GADEK. Induction of apoptosis in human cervix carcinoma cells during therapy by cisplatin. Cancer Detection and Prevention. USA: x, 2002, accepted, x, s. x, x. ISSN 0361-090X.

@article{483358,
   author = {Schloffer, Daniela and Horký, Marcel and Kotala, Vladimír and WesierskaandGadek, Jozefa},
   article_location = {USA},
   article_number = {x},
   keywords = {activated caspases; cytochrome C release; DNA replication; mitosis; nucleolar segregation; PARP-1 degradation},
   language = {eng},
   issn = {0361-090X},
   journal = {Cancer Detection and Prevention},
   title = {Induction of apoptosis in human cervix carcinoma cells during therapy by cisplatin},
   volume = {accepted},
   year = {2002}
}

TY  - JOUR
ID  - 483358
AU  - Schloffer, Daniela - Horký, Marcel - Kotala, Vladimír - Wesierska-Gadek, Jozefa
PY  - 2002
TI  - Induction of apoptosis in human cervix carcinoma cells during therapy by cisplatin
JF  - Cancer Detection and Prevention
VL  - accepted
IS  - x
SP  - x
EP  - x
PB  - x
SN  - 0361090X
KW  - activated caspases
KW  - cytochrome C release
KW  - DNA replication
KW  - mitosis
KW  - nucleolar segregation
KW  - PARP-1 degradation
N2  - The aim of the therapy of human malignancies is the inhibition of cell proliferation and/or induction of apoptosis. We studied the kinetics of the morphological and biochemical changes in HeLa cells during chemotherapy by cisplatin. Apoptosis was evaluated by scoring of cells exhibiting changes characteristic for early and late stages of apoptosis as determined by Hoehst 33258 staining and by examination of positive reaction for activated caspase-3. Expression and intracellular localization of distinct proteins eas analyzed by immunoblotting of subcellular fractions and segregation of nucleoli by immunocytochemistry.Chromatin fragmentation characteristic for apoptosis was observed in single cells after 3h cisplatin. A strong cytoplasmic accumulation of cytochrome C detected by immunoblotting 6h post-treatment was accompanied by an activation of caspase-9. Neither inhibition of cell division nor blocking of DNA replication preceded the onset of apoptosis, Our results show that after short treatment by cisplatin cell proliferation and apoptosis concomitantly occurred
ER  -

SCHLOFFER, Daniela, Marcel HORKÝ, Vladimír KOTALA a Jozefa WESIERSKA-GADEK. Induction of apoptosis in human cervix carcinoma cells during therapy by cisplatin. \textit{Cancer Detection and Prevention}. USA: x, 2002, accepted, x, s.~x, x. ISSN~0361-090X.

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