APOPTOSIS OF CARDIAC MYOCYTES AND ENDOTHELIAL CELLS CONTRIBUTES
TO PATHOGENESIS OF DOXORUBICIN-INDUCED CARDIOMYOPATHY IN
SPONTANEOUSLY HYPERTENSIVE RATS

J 2002

APOPTOSIS OF CARDIAC MYOCYTES AND ENDOTHELIAL CELLS CONTRIBUTES TO PATHOGENESIS OF DOXORUBICIN-INDUCED CARDIOMYOPATHY IN SPONTANEOUSLY HYPERTENSIVE RATS

UMLAUF, Jozef, Šárka KUCHTÍČKOVÁ, B. SLANÁ a Vladimír KOTALA

Základní údaje

Originální název

APOPTOSIS OF CARDIAC MYOCYTES AND ENDOTHELIAL CELLS CONTRIBUTES TO PATHOGENESIS OF DOXORUBICIN-INDUCED CARDIOMYOPATHY IN SPONTANEOUSLY HYPERTENSIVE RATS

Autoři

UMLAUF, Jozef (203 Česká republika, garant), Šárka KUCHTÍČKOVÁ (203 Česká republika), B. SLANÁ (203 Česká republika) a Vladimír KOTALA (203 Česká republika)

Vydání

Biomarkers and Enviroment, Česká republika, CECHTUMA, s.r.o. 2002, 1211-8869

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/02:00007253

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Doxorubicin; Apoptosis; Endothelial cells; Cardiac myocytes; Caspase-3; TUNEL assay

Štítky

apoptosis, cardiac myocytes, caspase-3, doxorubicin, endothelial cells, TUNEL assay

Změněno: 16. 1. 2003 17:04, Mgr. Vladimír Kotala, Ph.D.

Anotace

V originále

The aim of our study was to detect apoptosis of cardiac myocytes and endothelial cells of the left ventricular myocardium in SHR. We used the TUNEL assay and caspase-3 antibodies to assess critical steps in the pathways of active cell death, which involve rapid proteolysis and final DNA fragmentation. The spontaneously hypertensive rats were treated with doxorubucin for 8 weeks. The left ventricular myocardium was harvested after the 1st, 2nd, 3rd, 4th and 8th weeks. The histological examination revealed a rapid onset of nuclear caspase- 3 positivity as well as TUNEL positivity after 7 days of treatment, which continued in all the periods investigated. We also found caspase-3 positive nuclei in cardiac myocytes after the 1st week of doxorubicin treatment. The number of TUNEL positive cardiomyocytes rose after the second week of treatment and peaked after 4 weeks of treatment. Our data show that apoptosis of endothelial cells and cardiac myocytes plays a critical role in the pathogenesis of doxorubicin induced cardiomyopathy.

Návaznosti

MSM 141100002, záměr

Název: Molekulární patofyziologie multigenních chorob

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární patofyziologie multigenních chorob

Citovat

UMLAUF, Jozef, Šárka KUCHTÍČKOVÁ, B. SLANÁ a Vladimír KOTALA. APOPTOSIS OF CARDIAC MYOCYTES AND ENDOTHELIAL CELLS CONTRIBUTES TO PATHOGENESIS OF DOXORUBICIN-INDUCED CARDIOMYOPATHY IN SPONTANEOUSLY HYPERTENSIVE RATS. Biomarkers and Enviroment. Česká republika: CECHTUMA, s.r.o., 2002, roč. 5, č. 1, s. 32-34. ISSN 1211-8869.

@article{483362,
   author = {Umlauf, Jozef and Kuchtíčková, Šárka and Slaná, B. and Kotala, Vladimír},
   article_location = {Česká republika},
   article_number = {1},
   keywords = {Doxorubicin; Apoptosis; Endothelial cells; Cardiac myocytes; Caspase-3; TUNEL assay},
   language = {eng},
   issn = {1211-8869},
   journal = {Biomarkers and Enviroment},
   title = {APOPTOSIS OF CARDIAC MYOCYTES AND ENDOTHELIAL CELLS CONTRIBUTES TO PATHOGENESIS OF DOXORUBICIN-INDUCED CARDIOMYOPATHY IN SPONTANEOUSLY HYPERTENSIVE RATS},
   volume = {5},
   year = {2002}
}

TY  - JOUR
ID  - 483362
AU  - Umlauf, Jozef - Kuchtíčková, Šárka - Slaná, B. - Kotala, Vladimír
PY  - 2002
TI  - APOPTOSIS OF CARDIAC MYOCYTES AND ENDOTHELIAL CELLS CONTRIBUTES TO PATHOGENESIS OF DOXORUBICIN-INDUCED CARDIOMYOPATHY IN SPONTANEOUSLY HYPERTENSIVE RATS
JF  - Biomarkers and Enviroment
VL  - 5
IS  - 1
SP  - 32
EP  - 32
PB  - CECHTUMA, s.r.o.
SN  - 12118869
KW  - Doxorubicin
KW  - Apoptosis
KW  - Endothelial cells
KW  - Cardiac myocytes
KW  - Caspase-3
KW  - TUNEL assay
N2  - The aim of our study was to detect apoptosis of cardiac myocytes and endothelial cells of the left ventricular myocardium in SHR. We used the TUNEL assay and caspase-3 antibodies to assess critical steps in the pathways of active cell death, which involve rapid proteolysis and final DNA fragmentation. The spontaneously hypertensive rats were treated with doxorubucin for 8 weeks. The left ventricular myocardium was harvested after the 1st, 2nd, 3rd, 4th and 8th weeks. The histological examination revealed a rapid onset of nuclear caspase- 3 positivity as well as TUNEL positivity after 7 days of treatment, which continued in all the periods investigated. We also found caspase-3 positive nuclei in cardiac myocytes after the 1st week of doxorubicin treatment. The number of TUNEL positive cardiomyocytes rose after the second week of treatment and peaked after 4 weeks of treatment. Our data show that apoptosis of endothelial cells and cardiac myocytes plays a critical role in the pathogenesis of doxorubicin induced cardiomyopathy.
ER  -

UMLAUF, Jozef, Šárka KUCHTÍČKOVÁ, B. SLANÁ a Vladimír KOTALA. APOPTOSIS OF CARDIAC MYOCYTES AND ENDOTHELIAL CELLS CONTRIBUTES TO PATHOGENESIS OF DOXORUBICIN-INDUCED CARDIOMYOPATHY IN SPONTANEOUSLY HYPERTENSIVE RATS. \textit{Biomarkers and Enviroment}. Česká republika: CECHTUMA, s.r.o., 2002, roč.~5, č.~1, s.~32-34. ISSN~1211-8869.

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