Interaction of DNA with echinomycin at the mercury electrode
surface as detected by impedance and chronopotentiometric
measurements.

J 2002

Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements.

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN a Vladimír VETTERL

Základní údaje

Originální název

Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements.

Autoři

HASOŇ, Stanislav (203 Česká republika), Jakub DVOŘÁK (203 Česká republika), František JELEN (203 Česká republika) a Vladimír VETTERL (203 Česká republika, garant)

Vydání

Talanta, Elsevier Science, 2002, 0039-9140

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.054

Kód RIV

RIV/00216224:14310/02:00007360

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

electrochemical impedance spectroscopy; EIS; DNA; bis-intercalator; echinomycin;

Štítky

bis-intercalator, DNA, echinomycin, EIS, electrochemical impedance spectroscopy

Změněno: 27. 1. 2003 22:56, prof. RNDr. Vladimír Vetterl, DrSc.

Anotace

V originále

The capacitance measurements (dependence of the differential capacitance C of the electrode double layer on potential e, C-E curves, electrochemical impedance spectroscopy (EIS - frequency deoendence of the impedance Z of the electrode double layer) and constant current chronopotentiometry (depndence of dt/dE on potential E at constant current, chronopotentiometric stripping analysis - CPSA) have been used for electrochemical study of echinomycin and its interaction with single-stranded (ss) and double-stranded(ds) DNA at the hanging mercury drop electrode (HMDE). The capacitance measurement showed that echinomycin gives a pseudocapacitance redox peak strongly dependent on the a.c.voltage frequency at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. Similar results were obtained using CPSA masurements. Thus capacitance measurements and CPSA can distinguish with the aid of the bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the mercury electrode surface, which promise to use this technique for detection of dsDNA in hybridisation reaction.

Návaznosti

GV204/97/K084, projekt VaV

Název: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Grantová agentura ČR, Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

IAA4004002, projekt VaV

Název: Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Investor: Akademie věd ČR, Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Citovat

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN a Vladimír VETTERL. Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements. Talanta. Elsevier Science, 2002, roč. 56, č. 5, s. 905-913. ISSN 0039-9140.

@article{483756,
   author = {Hasoň, Stanislav and Dvořák, Jakub and Jelen, František and Vetterl, Vladimír},
   article_number = {5},
   keywords = {electrochemical impedance spectroscopy; EIS; DNA; bis-intercalator; echinomycin;},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements.},
   volume = {56},
   year = {2002}
}

TY  - JOUR
ID  - 483756
AU  - Hasoň, Stanislav - Dvořák, Jakub - Jelen, František - Vetterl, Vladimír
PY  - 2002
TI  - Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements.
JF  - Talanta
VL  - 56
IS  - 5
SP  - 905
EP  - 905
PB  - Elsevier Science
SN  - 00399140
KW  - electrochemical impedance spectroscopy
KW  - EIS
KW  - DNA
KW  - bis-intercalator
KW  - echinomycin;
N2  - The capacitance measurements (dependence of the differential capacitance C of the electrode double layer on potential e, C-E curves, electrochemical impedance spectroscopy (EIS - frequency deoendence of the impedance Z of the electrode double layer) and constant current chronopotentiometry (depndence of dt/dE on potential E at constant current, chronopotentiometric stripping analysis - CPSA) have been used for electrochemical study of echinomycin and its interaction with single-stranded (ss) and double-stranded(ds) DNA at the hanging mercury drop electrode (HMDE). The capacitance measurement showed that echinomycin gives a pseudocapacitance redox peak strongly dependent on the a.c.voltage frequency at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. Similar results were obtained using CPSA masurements. Thus capacitance measurements and CPSA can distinguish with the aid of the bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the mercury electrode surface, which promise to use this technique for detection of dsDNA in hybridisation reaction.
ER  -

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN a Vladimír VETTERL. Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements. \textit{Talanta}. Elsevier Science, 2002, roč.~56, č.~5, s.~905-913. ISSN~0039-9140.

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