Interaction of DNA with echinomycin at the mercury electrode
surface as detected by impedance and chronopotentiometric
measurements.

J 2002

Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements.

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN and Vladimír VETTERL

Basic information

Original name

Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements.

Authors

HASOŇ, Stanislav (203 Czech Republic), Jakub DVOŘÁK (203 Czech Republic), František JELEN (203 Czech Republic) and Vladimír VETTERL (203 Czech Republic, guarantor)

Edition

Talanta, Elsevier Science, 2002, 0039-9140

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.054

RIV identification code

RIV/00216224:14310/02:00007360

Organization unit

Faculty of Science

Keywords in English

electrochemical impedance spectroscopy; EIS; DNA; bis-intercalator; echinomycin;

Abstract

V originále

The capacitance measurements (dependence of the differential capacitance C of the electrode double layer on potential e, C-E curves, electrochemical impedance spectroscopy (EIS - frequency deoendence of the impedance Z of the electrode double layer) and constant current chronopotentiometry (depndence of dt/dE on potential E at constant current, chronopotentiometric stripping analysis - CPSA) have been used for electrochemical study of echinomycin and its interaction with single-stranded (ss) and double-stranded(ds) DNA at the hanging mercury drop electrode (HMDE). The capacitance measurement showed that echinomycin gives a pseudocapacitance redox peak strongly dependent on the a.c.voltage frequency at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. Similar results were obtained using CPSA masurements. Thus capacitance measurements and CPSA can distinguish with the aid of the bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the mercury electrode surface, which promise to use this technique for detection of dsDNA in hybridisation reaction.

Links

GV204/97/K084, research and development project

Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research

IAA4004002, research and development project

Name: Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Investor: Academy of Sciences of the Czech Republic, Structure and Interaction of Nucleic Acids on Metal Surfaces

Citovat

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN and Vladimír VETTERL. Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements. Talanta. Elsevier Science, 2002, vol. 56, No 5, p. 905-913. ISSN 0039-9140.

@article{483756,
   author = {Hasoň, Stanislav and Dvořák, Jakub and Jelen, František and Vetterl, Vladimír},
   article_number = {5},
   keywords = {electrochemical impedance spectroscopy; EIS; DNA; bis-intercalator; echinomycin;},
   language = {eng},
   issn = {0039-9140},
   journal = {Talanta},
   title = {Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements.},
   volume = {56},
   year = {2002}
}

TY  - JOUR
ID  - 483756
AU  - Hasoň, Stanislav - Dvořák, Jakub - Jelen, František - Vetterl, Vladimír
PY  - 2002
TI  - Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements.
JF  - Talanta
VL  - 56
IS  - 5
SP  - 905
EP  - 905
PB  - Elsevier Science
SN  - 00399140
KW  - electrochemical impedance spectroscopy
KW  - EIS
KW  - DNA
KW  - bis-intercalator
KW  - echinomycin;
N2  - The capacitance measurements (dependence of the differential capacitance C of the electrode double layer on potential e, C-E curves, electrochemical impedance spectroscopy (EIS - frequency deoendence of the impedance Z of the electrode double layer) and constant current chronopotentiometry (depndence of dt/dE on potential E at constant current, chronopotentiometric stripping analysis - CPSA) have been used for electrochemical study of echinomycin and its interaction with single-stranded (ss) and double-stranded(ds) DNA at the hanging mercury drop electrode (HMDE). The capacitance measurement showed that echinomycin gives a pseudocapacitance redox peak strongly dependent on the a.c.voltage frequency at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. Similar results were obtained using CPSA masurements. Thus capacitance measurements and CPSA can distinguish with the aid of the bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the mercury electrode surface, which promise to use this technique for detection of dsDNA in hybridisation reaction.
ER  -

HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN and Vladimír VETTERL. Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements. \textit{Talanta}. Elsevier Science, 2002, vol.~56, No~5, p.~905-913. ISSN~0039-9140.

Detailed Information on Publication Record