Determination of homocysteine in human plasma by mekc and
in-capillary detection reaction with 2,2-dipyridyldisulfide

J 2003

Determination of homocysteine in human plasma by mekc and in-capillary detection reaction with 2,2-dipyridyldisulfide

ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ a Josef TOMANDL

Základní údaje

Originální název

Determination of homocysteine in human plasma by mekc and in-capillary detection reaction with 2,2-dipyridyldisulfide

Autoři

ŠEVČÍKOVÁ, Petra (203 Česká republika), Zdeněk GLATZ (203 Česká republika, garant, domácí) a Josef TOMANDL (203 Česká republika, domácí)

Vydání

Journal of Chromatography A, Netherlands, Elsevier, 2003, 0021-9606

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.950

Kód RIV

RIV/00216224:14310/03:00007946

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000181647200022

Klíčová slova anglicky

Capillary electrophoresis; on-column derivatization; homocysteine

Štítky

Capillary electrophoresis, homocysteine, On-Column Derivatization

Změněno: 6. 6. 2012 08:30, doc. RNDr. Josef Tomandl, Ph.D.

Anotace

V originále

In this paper, we present a new method for homocysteine quantitation in human plasma based on in-capillary reaction of homocysteine with 2,2-dipyridyldisulfide. Homocysteine is in this so called thiol-exchange reaction quantitatively transformed in mixed disulfide concomitantly with formation of equimolar amount of 2-thiopyridone that is further separated by MEKC and determined specifically at 343 nm. The concentration of homocysteine is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay was from 0.03 to 3 mM (correlation coefficient 0.994) with a detection limit of 6 mM and a limit of quantitation 20 mM. The inter-day reproducibility of the peak area and the migration time were 1.37 % and 0.05 %, respectively. The method is simple, relatively rapid and can be easily automated. Moreover the common CE apparatus with UV detector can be used to distinguish between normal and pathological hyperhomocysteinemia plasma samples.

Návaznosti

GA203/02/1447, projekt VaV

Název: Kapilární elektrochromatografie v lineárních a planárních mikrostrukturách s využitím polymerních náplní

Investor: Grantová agentura ČR, Kapilární elektrochromatografie v lineárních a planárních mikrostrukturách s využitím polymerních náplní

GA203/03/1125, projekt VaV

Název: Využití kapilární zónové elektroforézy pro studium enzymů

Investor: Grantová agentura ČR, Využití kapilární zónové elektroforézy pro studium enzymů

Citovat

ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ a Josef TOMANDL. Determination of homocysteine in human plasma by mekc and in-capillary detection reaction with 2,2-dipyridyldisulfide. Journal of Chromatography A. Netherlands: Elsevier, 2003, roč. 990, (1+2), s. 197-204. ISSN 0021-9606.

@article{484268,
   author = {Ševčíková, Petra and Glatz, Zdeněk and Tomandl, Josef},
   article_location = {Netherlands},
   article_number = {(1+2)},
   keywords = {Capillary electrophoresis; on-column derivatization; homocysteine},
   language = {eng},
   issn = {0021-9606},
   journal = {Journal of Chromatography A},
   title = {Determination of homocysteine in human plasma by mekc and in-capillary detection reaction with 2,2-dipyridyldisulfide},
   volume = {990},
   year = {2003}
}

TY  - JOUR
ID  - 484268
AU  - Ševčíková, Petra - Glatz, Zdeněk - Tomandl, Josef
PY  - 2003
TI  - Determination of homocysteine in human plasma by mekc and in-capillary detection reaction with 2,2-dipyridyldisulfide
JF  - Journal of Chromatography A
VL  - 990
IS  - (1+2)
SP  - 197
EP  - 197
PB  - Elsevier
SN  - 00219606
KW  - Capillary electrophoresis
KW  - on-column derivatization
KW  - homocysteine
N2  - In this paper, we present a new method for homocysteine quantitation in human plasma based on in-capillary reaction of homocysteine with 2,2-dipyridyldisulfide. Homocysteine is in this so called thiol-exchange reaction quantitatively transformed in mixed disulfide concomitantly with formation of equimolar amount of 2-thiopyridone that is further separated by MEKC and determined specifically at 343 nm. The concentration of homocysteine is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay was from 0.03 to 3 mM (correlation coefficient 0.994) with a detection limit of 6 mM and a limit of quantitation 20 mM. The inter-day reproducibility of the peak area and the migration time were 1.37 % and 0.05 %, respectively. The method is simple, relatively rapid and can be easily automated. Moreover the common CE apparatus with UV detector can be used to distinguish between normal and pathological hyperhomocysteinemia plasma samples.
ER  -

ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ a Josef TOMANDL. Determination of homocysteine in human plasma by mekc and in-capillary detection reaction with 2,2-dipyridyldisulfide. \textit{Journal of Chromatography A}. Netherlands: Elsevier, 2003, roč.~990, (1+2), s.~197-204. ISSN~0021-9606.

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