Electrochemical impedance spectroscopy of polynucleotides.

D 2001

Electrochemical impedance spectroscopy of polynucleotides.

VETTERL, Vladimír, Luděk STRAŠÁK, Stanislav HASOŇ a Jakub DVOŘÁK

Základní údaje

Originální název

Electrochemical impedance spectroscopy of polynucleotides.

Autoři

VETTERL, Vladimír (203 Česká republika, garant), Luděk STRAŠÁK (203 Česká republika), Stanislav HASOŇ (203 Česká republika) a Jakub DVOŘÁK (203 Česká republika)

Vydání

Bratislava, Book of Abstracts, XVI th International Symposium on Bioelectrochemistry and Bioenergetics, s. 110-110, 2001

Nakladatel

XVI th International Symposium on Bioelectrochemistry and Bioenergetics

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10610 Biophysics

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/01:00007530

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

electrochemical impedance spectroscopy; EIS; DNA adsorption; polyadenylic acid adsorption; poly a adsorption;; differential capacitance of the electrode double layer;

Štítky

DNA adsorption, EIS, electrochemical impedance spectroscopy, poly a adsorption, polyadenylic acid adsorption

Změněno: 29. 5. 2003 10:49, prof. RNDr. Vladimír Vetterl, DrSc.

Anotace

V originále

The dependence of the impedance of the electrode double layer of mercury electrode on frequency around the potentials of the tensammetric peaks of single-stranded and double-helical polynucleotides and DNA was studied. From the frequency dependence of the impedance of the electrode double layer represented in a complex impedance plot (the imaginary component Z'' is plotted against the real component Z', Cole-Cole or Nyquist plot) the electric equivalent circuit of the electrode covered with adsorbed DNA layer was determined. It was concluded the desorption of denatured ssDNA is accompanied by higher dielectric losses than the desorption of native dsDNA. This can be explained by the higher flexibility of ssDNA compared to the dsDNA. The capacitance peak of single stranded poly A observed at pH 8 around -1.3 V splits at low frequencies in two peaks.

Návaznosti

GV204/97/K084, projekt VaV

Název: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Grantová agentura ČR, Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

IAA4004002, projekt VaV

Název: Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Investor: Akademie věd ČR, Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Citovat

VETTERL, Vladimír, Luděk STRAŠÁK, Stanislav HASOŇ a Jakub DVOŘÁK. Electrochemical impedance spectroscopy of polynucleotides. In Book of Abstracts, XVI th International Symposium on Bioelectrochemistry and Bioenergetics. Bratislava: XVI th International Symposium on Bioelectrochemistry and Bioenergetics, 2001, s. 110.

@inproceedings{484374,
   author = {Vetterl, Vladimír and Strašák, Luděk and Hasoň, Stanislav and Dvořák, Jakub},
   address = {Bratislava},
   booktitle = {Book of Abstracts, XVI th International Symposium on Bioelectrochemistry and Bioenergetics},
   keywords = {electrochemical impedance spectroscopy; EIS; DNA adsorption; polyadenylic acid adsorption; poly a adsorption;; differential capacitance of the electrode double layer;},
   language = {eng},
   location = {Bratislava},
   pages = {110-110},
   publisher = {XVI th International Symposium on Bioelectrochemistry and Bioenergetics},
   title = {Electrochemical impedance spectroscopy of polynucleotides.},
   year = {2001}
}

TY  - JOUR
ID  - 484374
AU  - Vetterl, Vladimír - Strašák, Luděk - Hasoň, Stanislav - Dvořák, Jakub
PY  - 2001
TI  - Electrochemical impedance spectroscopy of polynucleotides.
PB  - XVI th International Symposium on Bioelectrochemistry and Bioenergetics
CY  - Bratislava
KW  - electrochemical impedance spectroscopy
KW  - EIS
KW  - DNA adsorption
KW  - polyadenylic acid adsorption
KW  - poly a adsorption;
KW  - differential capacitance of the electrode double layer;
N2  - The dependence of the impedance of the electrode double layer of mercury electrode on frequency around the potentials of the tensammetric peaks of single-stranded and double-helical polynucleotides and DNA was studied. From the frequency dependence of the impedance of the electrode double layer represented in a complex impedance plot (the imaginary component Z'' is plotted against the real component Z', Cole-Cole or Nyquist plot) the electric equivalent circuit of the electrode covered with adsorbed DNA layer was determined. It was concluded the desorption of denatured ssDNA is accompanied by higher dielectric losses than the desorption of native dsDNA. This can be explained by the higher flexibility of ssDNA compared to the dsDNA. The capacitance peak of single stranded poly A observed at pH 8 around -1.3 V splits at low frequencies in two peaks.
ER  -

VETTERL, Vladimír, Luděk STRAŠÁK, Stanislav HASOŇ a Jakub DVOŘÁK. Electrochemical impedance spectroscopy of polynucleotides. In \textit{Book of Abstracts, XVI th International Symposium on Bioelectrochemistry and Bioenergetics}. Bratislava: XVI th International Symposium on Bioelectrochemistry and Bioenergetics, 2001, s.~110.

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