Alkylsulfonates as probes of uncoupling protein transport
mechanism: ion pair transport demonstrates that H+
translocation by UCP1 is not necessary for uncoupling

J 2001

Alkylsulfonates as probes of uncoupling protein transport mechanism: ion pair transport demonstrates that H+ translocation by UCP1 is not necessary for uncoupling

JABŮREK, M.; M. VAŘECHA; P. JEŽEK a K.D. GARLID

Základní údaje

Originální název

Alkylsulfonates as probes of uncoupling protein transport mechanism: ion pair transport demonstrates that H+ translocation by UCP1 is not necessary for uncoupling

Název česky

Alkylsulfonáty jako sondy transportního mechanismu odpřahujícího proteinu: transport iontových páru demonstruje, že přenos H+ pomocí UCP1 není nezbytný pro odpřažení

Autoři

JABŮREK, M.; M. VAŘECHA; P. JEŽEK a K.D. GARLID

Vydání

Journal of Biological Chemistry, Bethesda, USA, Amer. Soc. Biochem. Mol. Biol. 2001, 0021-9258

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 7.258

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14330/01:00030508

Organizační jednotka

Fakulta informatiky

UT WoS

000170613500060

Klíčová slova anglicky

UCP1;uncoupling protein;mitochondria

Štítky

mitochondria, UCP1, uncoupling protein

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 6. 2010 11:59, Mgr. Miroslav Vařecha, Ph.D.

Anotace

ORIG CZ

V originále

The mechanism of fatty acid-dependent uncoupling by mitochondrial uncoupling proteins (UCP) is still in debate. We have hypothesized that the anionic fatty acid head group is translocated by UCP, and the proton is transported electroneutrally in the bilayer by flip-flop of the protonated fatty acid. Alkylsulfonates are useful as probes of the UCP transport mechanism. They are analogues of fatty acids, and they are transported by UCP1, UCP2, and UCP3. We show that undecanesulfonate and laurate are mutually competitive inhibitors, supporting the hypothesis that fatty acid anion is transported by UCP1. Alkylsulfonates cannot be protonated because of their low pK(a), consequently, they cannot catalyze electroneutral proton transport in the bilayer and cannot support uncoupling by UCP. We report for the first time that propranolol forms permeant ion pairs with the alkylsulfonates, thereby removing this restriction. Because a proton is transported with the neutral ion pair, the sulfonate is able to deliver protons across the bilayer, behaving as if it were a fatty acid. When ion pair transport is combined with UCP1, we now observe electrophoretic proton transport and uncoupling of brown adipose tissue mitochondria. These experiments confirm that the proton transport of UCP-mediated uncoupling takes place in the lipid bilayer and not via UCP itself. Thus, UCP1, like other members of its gene family, translocates anions and does not translocate protons.

Česky

Alkylsulfonáty jako sondy transportního mechanismu odpřahujícího proteinu: transport iontových páru demonstruje, že přenos H+ pomocí UCP1 není nezbytný pro odpřažení.

Citovat

JABŮREK, M.; M. VAŘECHA; P. JEŽEK a K.D. GARLID. Alkylsulfonates as probes of uncoupling protein transport mechanism: ion pair transport demonstrates that H+ translocation by UCP1 is not necessary for uncoupling. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2001, roč. 276, č. 34, s. 31897-31905. ISSN 0021-9258.

@article{485152,
   author = {Jabůrek, M. and Vařecha, M. and Ježek, P. and Garlid, K.D.},
   article_location = {Bethesda, USA},
   article_number = {34},
   keywords = {UCP1;uncoupling protein;mitochondria},
   language = {eng},
   issn = {0021-9258},
   journal = {Journal of Biological Chemistry},
   title = {Alkylsulfonates as probes of uncoupling protein transport mechanism: ion pair transport demonstrates that H+ translocation by UCP1 is not necessary for uncoupling},
   volume = {276},
   year = {2001}
}

TY  - JOUR
ID  - 485152
AU  - Jabůrek, M. - Vařecha, M. - Ježek, P. - Garlid, K.D.
PY  - 2001
TI  - Alkylsulfonates as probes of uncoupling protein transport mechanism: ion pair transport demonstrates that H+ translocation by UCP1 is not necessary for uncoupling
JF  - Journal of Biological Chemistry
VL  - 276
IS  - 34
SP  - 31897-31905
EP  - 31897-31905
PB  - Amer. Soc. Biochem. Mol. Biol.
SN  - 00219258
KW  - UCP1;uncoupling protein;mitochondria
N2  - The mechanism of fatty acid-dependent uncoupling by mitochondrial uncoupling proteins (UCP) is still in debate. We have hypothesized that the anionic fatty acid head group is translocated by UCP, and the proton is transported electroneutrally in the bilayer by flip-flop of the protonated fatty acid. Alkylsulfonates are useful as probes of the UCP transport mechanism. They are analogues of fatty acids, and they are transported by UCP1, UCP2, and UCP3. We show that undecanesulfonate and laurate are mutually competitive inhibitors, supporting the hypothesis that fatty acid anion is transported by UCP1. Alkylsulfonates cannot be protonated because of their low pK(a), consequently, they cannot catalyze electroneutral proton transport in the bilayer and cannot support uncoupling by UCP. We report for the first time that propranolol forms permeant ion pairs with the alkylsulfonates, thereby removing this restriction. Because a proton is transported with the neutral ion pair, the sulfonate is able to deliver protons across the bilayer, behaving as if it were a fatty acid. When ion pair transport is combined with UCP1, we now observe electrophoretic proton transport and uncoupling of brown adipose tissue mitochondria. These experiments confirm that the proton transport of UCP-mediated uncoupling takes place in the lipid bilayer and not via UCP itself. Thus, UCP1, like other members of its gene family, translocates anions and does not translocate protons.
ER  -

JABŮREK, M.; M. VAŘECHA; P. JEŽEK a K.D. GARLID. Alkylsulfonates as probes of uncoupling protein transport mechanism: ion pair transport demonstrates that H+ translocation by UCP1 is not necessary for uncoupling. \textit{Journal of Biological Chemistry}. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2001, roč.~276, č.~34, s.~31897-31905. ISSN~0021-9258.

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