Nucleolar segregation coincides with nuclear accumulation of
death domain in neurons undergoing apoptosis induced by
ischemia reperfusion injury

J 2003

Nucleolar segregation coincides with nuclear accumulation of death domain in neurons undergoing apoptosis induced by ischemia reperfusion injury

HORKÝ, Marcel, Martin SMRČKA, Filip OTEVŘEL, Šárka KUCHTÍČKOVÁ, Vilém JURÁŇ et. al.

Basic information

Original name

Nucleolar segregation coincides with nuclear accumulation of death domain in neurons undergoing apoptosis induced by ischemia reperfusion injury

Authors

HORKÝ, Marcel (203 Czech Republic, guarantor), Martin SMRČKA (203 Czech Republic), Filip OTEVŘEL (203 Czech Republic), Šárka KUCHTÍČKOVÁ (203 Czech Republic), Vilém JURÁŇ (203 Czech Republic), M. DUBA (203 Czech Republic) and Jan MUŽÍK (203 Czech Republic)

Edition

Physiological Research, Praha, Academy of Sciences of the Czech Rep. 2003, 0862-8408

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.939

RIV identification code

RIV/00216224:14110/03:00008625

Organization unit

Faculty of Medicine

Keywords in English

apoptosis; caspase-3; nucleoli; MADD

Abstract

V originále

We focused on histochemical detection of distribution of NOR (argyrophylic nucleolar proteins) reflecting nucleolar integrity, immunohistochemical detection of MADD (mitogen activated death domain), a protein accumulated in nucleoli upon stimulation by ischemia and active form of caspase-3, a universal proteolytic enzyme of apoptosis. The terminal deoxynucleotidyl- transferase (TdT)-mediated dUTP-biotin nick-end-labeling method (TUNEL) proved the presence of in situ DNA fragmentation. We used the model of transient focal cerebral ischemia in rats. The period of ischemia lasted 15, 30, 60 and 120 minutes followed by 48 hrs of reperfusion. We examined the frontal lobe of the ipsilateral hemisphere. We found disintegrated nucleoli in all investigated periods of ischemia in cortical as well as subcortical neurons whereas the neurons of intact animals showed compact nucleoli with a few satellites. Nuclear positivity for MADD was apparent after 15 min. in neocortex and peaked after 30 min. of ischemia. On the other hand, the subcortical neurons showed nuclear positivity for MADD after 60 and 120 minutes. The intact brain tissue showed negative nuclei with only a slight cytoplasmic staining. The immunohistochemical reaction for active caspase 3 was apparent after 30 minutes onwards predominantly in cortex. The controls were caspase 3 negative. The TUNEL staining was remarkable after 60 and 120 minutes. We detected a rapid onset of mucleolar segregation in cortical and subcortical neurons damaged by ischemia (15 min) reperfusion injury which coincides with a nuclear/nucleolar accumulation of MADD, a stress activated neuron specific death domain. Active caspase 3 and TUNEL positivity were found after a prolonged ischemia (30, 60 and 120 min.)

Links

MSM 141100002, plan (intention)

Name: Molekulární patofyziologie multigenních chorob

Investor: Ministry of Education, Youth and Sports of the CR, Molecular pathophysiology of multigene diseases

Citovat

HORKÝ, Marcel, Martin SMRČKA, Filip OTEVŘEL, Šárka KUCHTÍČKOVÁ, Vilém JURÁŇ, M. DUBA and Jan MUŽÍK. Nucleolar segregation coincides with nuclear accumulation of death domain in neurons undergoing apoptosis induced by ischemia reperfusion injury. Physiological Research. Praha: Academy of Sciences of the Czech Rep., 2003, xxx, xx, p. xxx, 5-in print. ISSN 0862-8408.

@article{485308,
   author = {Horký, Marcel and Smrčka, Martin and Otevřel, Filip and Kuchtíčková, Šárka and Juráň, Vilém and Duba, M. and Mužík, Jan},
   article_location = {Praha},
   article_number = {xx},
   keywords = {apoptosis; caspase-3; nucleoli; MADD},
   language = {eng},
   issn = {0862-8408},
   journal = {Physiological Research},
   title = {Nucleolar segregation coincides with nuclear accumulation of death domain in neurons undergoing apoptosis induced by ischemia reperfusion injury},
   volume = {xxx},
   year = {2003}
}

TY  - JOUR
ID  - 485308
AU  - Horký, Marcel - Smrčka, Martin - Otevřel, Filip - Kuchtíčková, Šárka - Juráň, Vilém - Duba, M. - Mužík, Jan
PY  - 2003
TI  - Nucleolar segregation coincides with nuclear accumulation of death domain in neurons undergoing apoptosis induced by ischemia reperfusion injury
JF  - Physiological Research
VL  - xxx
IS  - xx
SP  - xxx
EP  - xxx
PB  - Academy of Sciences of the Czech Rep.
SN  - 08628408
KW  - apoptosis
KW  - caspase-3
KW  - nucleoli
KW  - MADD
N2  - We focused on histochemical detection of distribution of NOR (argyrophylic nucleolar proteins) reflecting nucleolar integrity, immunohistochemical detection of MADD (mitogen activated death domain), a protein accumulated in nucleoli upon stimulation by ischemia and active form of caspase-3, a universal proteolytic enzyme of apoptosis. The terminal deoxynucleotidyl- transferase (TdT)-mediated dUTP-biotin nick-end-labeling method (TUNEL) proved the presence of in situ DNA fragmentation. We used the model of transient focal cerebral ischemia in rats. The period of ischemia lasted 15, 30, 60 and 120 minutes followed by 48 hrs of reperfusion. We examined the frontal lobe of the ipsilateral hemisphere. We found disintegrated nucleoli in all investigated periods of ischemia in cortical as well as subcortical neurons whereas the neurons of intact animals showed compact nucleoli with a few satellites. Nuclear positivity for MADD was apparent after 15 min. in neocortex and peaked after 30 min. of ischemia. On the other hand, the subcortical neurons showed nuclear positivity for MADD after 60 and 120 minutes. The intact brain tissue showed negative nuclei with only a slight cytoplasmic staining. The immunohistochemical reaction for active caspase 3 was apparent after 30 minutes onwards predominantly in cortex. The controls were caspase 3 negative. The TUNEL staining was remarkable after 60 and 120 minutes. We detected a rapid onset of mucleolar segregation in cortical and subcortical neurons damaged by ischemia (15 min) reperfusion injury which coincides with a nuclear/nucleolar accumulation of MADD, a stress activated neuron specific death domain. Active caspase 3 and TUNEL positivity were found after a prolonged ischemia (30, 60 and 120 min.)
ER  -

HORKÝ, Marcel, Martin SMRČKA, Filip OTEVŘEL, Šárka KUCHTÍČKOVÁ, Vilém JURÁŇ, M. DUBA and Jan MUŽÍK. Nucleolar segregation coincides with nuclear accumulation of death domain in neurons undergoing apoptosis induced by ischemia reperfusion injury. \textit{Physiological Research}. Praha: Academy of Sciences of the Czech Rep., 2003, xxx, xx, p.~xxx, 5-in print. ISSN~0862-8408.

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