Differential roles of Fos and Jun proteins in regulation of
differentiation and apoptosis of v-myb-transformed monoblasts

D 2003

Differential roles of Fos and Jun proteins in regulation of differentiation and apoptosis of v-myb-transformed monoblasts

ZAHRADNÍČKOVÁ, Eva, Sabina ŠEVČÍKOVÁ, Karel SOUČEK, Lukáš KUBALA, Jan ŠMARDA et. al.

Základní údaje

Originální název

Differential roles of Fos and Jun proteins in regulation of differentiation and apoptosis of v-myb-transformed monoblasts

Autoři

ZAHRADNÍČKOVÁ, Eva (203 Česká republika), Sabina ŠEVČÍKOVÁ (203 Česká republika), Karel SOUČEK (203 Česká republika), Lukáš KUBALA (203 Česká republika) a Jan ŠMARDA (203 Česká republika, garant)

Vydání

Silverthorne, USA, Antibody-based therapeutics for cancer, s. 62-62, 2003

Nakladatel

Keystone Symposia

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Kanada

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/03:00008028

Organizační jednotka

Přírodovědecká fakulta

ISBN

970-262-1230

Klíčová slova anglicky

Fos; Jun; apoptosis; differentiation; monoblast

Štítky

apoptosis, differentiation, Fos, Jun, monoblast

Změněno: 14. 5. 2003 15:18, prof. RNDr. Jan Šmarda, CSc.

Anotace

V originále

The line of v-myb-transformed chicken monoblasts BM2 constitutively express v-myb oncogene of AMV. v-Myb causes differentiation block, high proliferation rate and resistance to apoptosis similarly as in v-myb-transformed myeloid precursor primary cells. Differentiation capability of BM2 cells to macrophage-like cells can resume by phorbol esters that activate PKC and transcription from promoters containing TRE. The fact that transcription factor AP-1 binds TRE prompted us to investigate the role of individual components of AP-1 - the Jun and Fos proteins in regulation of differentiation, proliferation and apoptosis of BM2 cells. We ectopically expressed c-Jun, c-Fos, v-Jun and v-Fos proteins in BM2 cells and compared their effects. We found that c-Fos but not c-Jun protein was capable to induce apoptosis of BM2 cells. Interestingly, transforming variant of the Fos protein (v-Fos) lost capacity of its cellular precursor to cause apoptosis of BM2 cells. On contrary, c-Jun but not c-Fos protein induced differentiation of BM2 cells to adherent macrophage-like cells. Both Fos and Jun proteins down-regulated proliferation of BM2 cells suggesting that individual components of AP-1 transcription factor possess distinct roles in determination of cell afte. Therefore, the presence of both Jun and Fos proteins in the cell seems to be optimal for tumor-suppressive processes.

Návaznosti

GA301/01/0040, projekt VaV

Název: Úloha proteinů Fos a Jun v monocytické diferenciaci stimulované kyselinou retinovou

Investor: Grantová agentura ČR, Úloha proteinů Fos a Jun v monocytické diferenciaci stimulované kyselinou retinovou

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

Citovat

ZAHRADNÍČKOVÁ, Eva, Sabina ŠEVČÍKOVÁ, Karel SOUČEK, Lukáš KUBALA a Jan ŠMARDA. Differential roles of Fos and Jun proteins in regulation of differentiation and apoptosis of v-myb-transformed monoblasts. In Antibody-based therapeutics for cancer. Silverthorne, USA: Keystone Symposia, 2003, s. 62. ISBN 970-262-1230.

@inproceedings{486513,
   author = {Zahradníčková, Eva and Ševčíková, Sabina and Souček, Karel and Kubala, Lukáš and Šmarda, Jan},
   address = {Silverthorne, USA},
   booktitle = {Antibody-based therapeutics for cancer},
   keywords = {Fos; Jun; apoptosis; differentiation; monoblast},
   language = {eng},
   location = {Silverthorne, USA},
   isbn = {970-262-1230},
   pages = {62-62},
   publisher = {Keystone Symposia},
   title = {Differential roles of Fos and Jun proteins in regulation of differentiation and apoptosis of v-myb-transformed monoblasts},
   year = {2003}
}

TY  - JOUR
ID  - 486513
AU  - Zahradníčková, Eva - Ševčíková, Sabina - Souček, Karel - Kubala, Lukáš - Šmarda, Jan
PY  - 2003
TI  - Differential roles of Fos and Jun proteins in regulation of differentiation and apoptosis of v-myb-transformed monoblasts
PB  - Keystone Symposia
CY  - Silverthorne, USA
SN  - 9702621230
KW  - Fos
KW  - Jun
KW  - apoptosis
KW  - differentiation
KW  - monoblast
N2  - The line of v-myb-transformed chicken monoblasts BM2 constitutively express v-myb oncogene of AMV. v-Myb causes differentiation block, high proliferation rate and resistance to apoptosis similarly as in v-myb-transformed myeloid precursor primary cells. Differentiation capability of BM2 cells to macrophage-like cells can resume by phorbol esters that activate PKC and transcription from promoters containing TRE. The fact that transcription factor AP-1 binds TRE prompted us to investigate the role of individual components of AP-1 - the Jun and Fos proteins in regulation of differentiation, proliferation and apoptosis of BM2 cells. We ectopically expressed c-Jun, c-Fos, v-Jun and v-Fos proteins in BM2 cells and compared their effects. We found that c-Fos but not c-Jun protein was capable to induce apoptosis of BM2 cells. Interestingly, transforming variant of the Fos protein (v-Fos) lost capacity of its cellular precursor to cause apoptosis of BM2 cells. On contrary, c-Jun but not c-Fos protein induced differentiation of BM2 cells to adherent macrophage-like cells. Both Fos and Jun proteins down-regulated proliferation of BM2 cells suggesting that individual components of AP-1 transcription factor possess distinct roles in determination of cell afte. Therefore, the presence of both Jun and Fos proteins in the cell seems to be optimal for tumor-suppressive processes.
ER  -

ZAHRADNÍČKOVÁ, Eva, Sabina ŠEVČÍKOVÁ, Karel SOUČEK, Lukáš KUBALA a Jan ŠMARDA. Differential roles of Fos and Jun proteins in regulation of differentiation and apoptosis of v-myb-transformed monoblasts. In \textit{Antibody-based therapeutics for cancer}. Silverthorne, USA: Keystone Symposia, 2003, s.~62. ISBN~970-262-1230.

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