LIGNANS OF SCHISANDRA CHINENSIS: ANALYSIS IN PLANT CELL CULTURE
AND ANTIPROLIFERATIVE ACTIVITY

B 2003

LIGNANS OF SCHISANDRA CHINENSIS: ANALYSIS IN PLANT CELL CULTURE AND ANTIPROLIFERATIVE ACTIVITY

SLANINA, Jiří; Iva SLANINOVÁ; Helena VLAŠÍNOVÁ; A. SMÍŠKOVÁ; Eva TÁBORSKÁ et al.

Základní údaje

Originální název

LIGNANS OF SCHISANDRA CHINENSIS: ANALYSIS IN PLANT CELL CULTURE AND ANTIPROLIFERATIVE ACTIVITY

Autoři

SLANINA, Jiří; Iva SLANINOVÁ; Helena VLAŠÍNOVÁ; A. SMÍŠKOVÁ a Eva TÁBORSKÁ

Vydání

Dusseldorf, 1 s. Phytochemistry and Biology of Lignans, 2003

Nakladatel

The Phytochemical Society of Europe

Další údaje

Jazyk

angličtina

Typ výsledku

Odborná kniha

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/03:00008061

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

lignans - Schisandra - plant cell culture - dibenzocyclooctadiene

Změněno: 29. 5. 2003 17:24, Mgr. Jiří Slanina, Ph.D.

Anotace

V originále

Dibenzocyclo[a,c]diene lignans occur exclusively in genera Schisandra and Kadsura (Schisandraceae/Magnoliaceae). Schisnadra chinensis is well-known medicinal plants of traditional Chinese medicine in which fruits have been used as tonic and antitusic. These lignans have been shown to possess a broad range of biological effects, including antivirral, antioxidative and hepatoprotective activities. Content of lignans in fruits of the plants is relatively low (~1 %) and is composed of at least thirty lignans. Moreover, contents of individual lignans considerably vary from plant to plant. Plant cell culture may represent a solution of the supply of these lignans. We determined the production of lignans in cell culture of S. chinensis using HPLC. Our preliminary results showed, that amount of lignans in plant cell cultures was lower than that in fruits and leaves. The main lignan in samples of cell cultures was deoxyschizandrin, its maximum yield reached 0.13 % based on biomass dry weight. The concentration of dexyschizandrin in plant cell culture was 10 times lower than that in fruits and 3 times lower than that in leaves. The antiproliferative activity of lignans against HeLa cells was evaluated using the neutral red uptake assay. The nonpolar lignans with S-biaryl configuration, gomisin N and wuwezisu C, were found to be more cytotoxic than schizandrin and gomisin A, somewhat polar lignans with R-biaryl configuration. Gomisin N displayed the highest cytotoxicity of the tested lignans with IC50 37 mg/L. To learn the possible effects of gomisin N on the architecture of cytoskeleton, actin fibers and microtubules were visualized using fluorescence staining. Exposure of the HeLa cells to gomisin N induced a reorganization of the cytoskeleton with the reduction of microtubules and aggregation of actin at the cell periphery

Návaznosti

GA521/02/1129, projekt VaV

Název: Využití biotechnologických metod k produkci sekundárních metabolitů, šlechtění a mikropropagaci léčivé rostliny klanoprašky čínské

Investor: Grantová agentura ČR, Využití biotechnologických metod k produkci sekundárních metabolitů, šlechtění a mikropropagaci léčivé rostliny klanoprašky čínské

Citovat

SLANINA, Jiří; Iva SLANINOVÁ; Helena VLAŠÍNOVÁ; A. SMÍŠKOVÁ a Eva TÁBORSKÁ. LIGNANS OF SCHISANDRA CHINENSIS: ANALYSIS IN PLANT CELL CULTURE AND ANTIPROLIFERATIVE ACTIVITY. Dusseldorf: The Phytochemical Society of Europe, 2003, 1 s. Phytochemistry and Biology of Lignans.

@book{487187,
   author = {Slanina, Jiří and Slaninová, Iva and Vlašínová, Helena and Smíšková, A. and Táborská, Eva},
   address = {Dusseldorf},
   keywords = {lignans - Schisandra - plant cell culture - dibenzocyclooctadiene},
   language = {eng},
   location = {Dusseldorf},
   publisher = {The Phytochemical Society of Europe},
   title = {LIGNANS OF SCHISANDRA CHINENSIS: ANALYSIS IN PLANT CELL CULTURE AND ANTIPROLIFERATIVE ACTIVITY},
   year = {2003}
}

TY  - BOOK
ID  - 487187
AU  - Slanina, Jiří - Slaninová, Iva - Vlašínová, Helena - Smíšková, A. - Táborská, Eva
PY  - 2003
TI  - LIGNANS OF SCHISANDRA CHINENSIS: ANALYSIS IN PLANT CELL CULTURE AND ANTIPROLIFERATIVE ACTIVITY
VL  - Phytochemistry and Biology of Lignans
PB  - The Phytochemical Society of Europe
CY  - Dusseldorf
KW  - lignans - Schisandra - plant cell culture - dibenzocyclooctadiene
N2  - Dibenzocyclo[a,c]diene lignans occur exclusively in genera Schisandra and Kadsura (Schisandraceae/Magnoliaceae). Schisnadra chinensis is well-known medicinal plants of traditional Chinese medicine in which fruits have been used as tonic and antitusic. These lignans have been shown to possess a broad range of biological effects, including antivirral, antioxidative and hepatoprotective activities. Content of lignans in fruits of the plants is relatively low (~1 %) and is composed of at least thirty lignans. Moreover, contents of individual lignans considerably vary from plant to plant. Plant cell culture may represent a solution of the supply of these lignans. We determined the production of lignans in cell culture of S. chinensis using HPLC. Our preliminary results showed, that amount of lignans in plant cell cultures was lower than that in fruits and leaves. The main lignan in samples of cell cultures was deoxyschizandrin, its maximum yield reached 0.13 % based on biomass dry weight. The concentration of dexyschizandrin in plant cell culture was 10 times lower than that in fruits and 3 times lower than that in leaves. The antiproliferative activity of lignans against HeLa cells was evaluated using the neutral red uptake assay. The nonpolar lignans with S-biaryl configuration, gomisin N and wuwezisu C, were found to be more cytotoxic than schizandrin and gomisin A, somewhat polar lignans with R-biaryl configuration. Gomisin N displayed the highest cytotoxicity of the tested lignans with IC50 37 mg/L. To learn the possible effects of gomisin N on the architecture of cytoskeleton, actin fibers and microtubules were visualized using fluorescence staining. Exposure of the HeLa cells to gomisin N induced a reorganization of the cytoskeleton with the reduction of microtubules and aggregation of actin at the cell periphery
ER  -

SLANINA, Jiří; Iva SLANINOVÁ; Helena VLAŠÍNOVÁ; A. SMÍŠKOVÁ a Eva TÁBORSKÁ. \textit{LIGNANS OF SCHISANDRA CHINENSIS: ANALYSIS IN PLANT CELL CULTURE AND ANTIPROLIFERATIVE ACTIVITY}. Dusseldorf: The Phytochemical Society of Europe, 2003, 1 s. Phytochemistry and Biology of Lignans.

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