2003
Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography
ŠEVČÍKOVÁ, Petra a Zdeněk GLATZZákladní údaje
Originální název
Specific determination of cysteine in human urine by capillary micellar electrokinetic chromatography
Autoři
ŠEVČÍKOVÁ, Petra (203 Česká republika) a Zdeněk GLATZ (203 Česká republika, garant)
Vydání
Journal of Separation Science, Weinheim, WILEY-VCH Verlag GmbH & Co. 2003, 1615-9306
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Německo
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/03:00008072
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000183660100015
Klíčová slova anglicky
urine; cysteine; MEKC; on-column reaction
Štítky
Změněno: 19. 5. 2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.
Anotace
V originále
This paper describes a method for the analysis of cysteine in human urine using capillary micellar electrokinetic chromatography and on-column reaction with 2,2-dipyridyl disulfide. In this reaction cysteine is quantitatively transformed into a mixed disulfide concomitantly with formation of an equimolar amount of 2-thiopyridone that is further separated by capillary micellar electrokinetic chromatography and determined spectrophotometrically at 343 nm. The concentration of cysteine is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay is from 0.05 to 5 mM (correlation coefficient 0.989) with a detection limit of 2.5 M and a limit of quantitation of 8.5 M. The inter-day reproducibility of the peak area was 2.18% and the inter-day reproducibility of the migration time 0.51%. The method is relatively rapid, simple, and can be easily automated. Moreover, its detection limit covers the concentration range at which cysteine is present in biological samples such as human urine.
Návaznosti
| GA203/03/1125, projekt VaV |
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