Identification of European diplozoids (Diplozoidae, Monogenea)
by restriction digestion of the ribosomal RNA internal
transcribed spacer (ITS2)

D 2003

Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2)

MATĚJUSOVÁ, Iveta, Božena KOUBKOVÁ a Carey O. CUNNINGHAM

Základní údaje

Originální název

Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2)

Autoři

MATĚJUSOVÁ, Iveta (203 Česká republika, garant), Božena KOUBKOVÁ (203 Česká republika) a Carey O. CUNNINGHAM (826 Velká Británie a Severní Irsko)

Vydání

Bergen, Norway, Bulletin of the Scandinavian Society for Parasitology, Proceedings of the XXI. Symposium of the Scandinavian Socity for Parasitology, Bergen, Norway 12-15 June, 2003, od s. 44-45, 2 s. 2003

Nakladatel

HS-Trykk A/S, Norway

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Norsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/03:00008781

Organizační jednotka

Přírodovědecká fakulta

ISBN

0803-4907

Klíčová slova anglicky

monogenea; diplozoids; PCR; RFLP; dignostic

Štítky

dignostic, diplozoids, Monogenea, PCR, RFLP

Změněno: 5. 9. 2003 13:18, Mgr. Iveta Matějusová, Ph.D.

Anotace

V originále

The second internal transcribed spacer (ITS2) of ribosomal DNA of diplozoid parasites was amplified, sequenced and restriction enzyme digestion was performed to reveal restriction fragment length polymorphism (RFLP). Genetic markers for 8 species of European diplozoids were developed. As those diplozoids are very closely related, it was very difficult to find suitable enzymes to reveal different patterns for each species. Only a combination of digestion with AluI, HaeIII, HinfI, RsaI and SphI proved convenient to discriminate all species studied. Morphological analysis and measurements of the attachment apparatus of the studied species are also presented, together with comparison of the shape of trapeze spur and the anterior joining sclerites.

Návaznosti

MSM 143100010, záměr

Název: Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy

Citovat

MATĚJUSOVÁ, Iveta, Božena KOUBKOVÁ a Carey O. CUNNINGHAM. Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2). In Bulletin of the Scandinavian Society for Parasitology, Proceedings of the XXI. Symposium of the Scandinavian Socity for Parasitology, Bergen, Norway 12-15 June, 2003. Bergen, Norway: HS-Trykk A/S, Norway, 2003, s. 44-45. ISBN 0803-4907.

@inproceedings{487541,
   author = {Matějusová, Iveta and Koubková, Božena and Cunningham, Carey O.},
   address = {Bergen, Norway},
   booktitle = {Bulletin of the Scandinavian Society for Parasitology, Proceedings of the XXI. Symposium of the Scandinavian Socity for Parasitology, Bergen, Norway 12-15 June, 2003},
   keywords = {monogenea; diplozoids; PCR; RFLP; dignostic},
   language = {eng},
   location = {Bergen, Norway},
   isbn = {0803-4907},
   pages = {44-45},
   publisher = {HS-Trykk A/S, Norway},
   title = {Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2)},
   year = {2003}
}

TY  - JOUR
ID  - 487541
AU  - Matějusová, Iveta - Koubková, Božena - Cunningham, Carey O.
PY  - 2003
TI  - Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2)
PB  - HS-Trykk A/S, Norway
CY  - Bergen, Norway
SN  - 08034907
KW  - monogenea
KW  - diplozoids
KW  - PCR
KW  - RFLP
KW  - dignostic
N2  - The second internal transcribed spacer (ITS2) of ribosomal DNA of diplozoid parasites was amplified, sequenced and restriction enzyme digestion was performed to reveal restriction fragment length polymorphism (RFLP). Genetic markers for 8 species of European diplozoids were developed. As those diplozoids are very closely related, it was very difficult to find suitable enzymes to reveal different patterns for each species. Only a combination of digestion with AluI, HaeIII, HinfI, RsaI and SphI proved convenient to discriminate all species studied. Morphological analysis and measurements of the attachment apparatus of the studied species are also presented, together with comparison of the shape of trapeze spur and the anterior joining sclerites.
ER  -

MATĚJUSOVÁ, Iveta, Božena KOUBKOVÁ a Carey O. CUNNINGHAM. Identification of European diplozoids (Diplozoidae, Monogenea) by restriction digestion of the ribosomal RNA internal transcribed spacer (ITS2). In \textit{Bulletin of the Scandinavian Society for Parasitology, Proceedings of the XXI. Symposium of the Scandinavian Socity for Parasitology, Bergen, Norway 12-15 June, 2003}. Bergen, Norway: HS-Trykk A/S, Norway, 2003, s.~44-45. ISBN~0803-4907.

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