Proteomic Analysis of Therapeutically Important Bacteriophage
812 by Combination of Electrophoresis and Mass Spectrometry

D 2003

Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry

PREISLER, Jan, Zbyněk ZDRÁHAL, Karel KLEPÁRNÍK, František FORET, Patrik VRÁBEL et. al.

Základní údaje

Originální název

Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry

Autoři

PREISLER, Jan (203 Česká republika, garant), Zbyněk ZDRÁHAL (203 Česká republika), Karel KLEPÁRNÍK (203 Česká republika), František FORET (203 Česká republika), Patrik VRÁBEL (203 Česká republika), Zdena MALÁ (203 Česká republika), Jiří DOŠKAŘ (203 Česká republika), Roman PANTŮČEK (203 Česká republika), Vladislava RŮŽIČKOVÁ (203 Česká republika), Hana KONEČNÁ (203 Česká republika) a Pavel KRÁSENSKÝ (203 Česká republika)

Vydání

Montreal, Proceedings of the 51st Annual Conference on Mass Spectrometry and Allied Topics, s. 1152-1152, 2003

Nakladatel

American Society for Mass Spectrometry

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

Genetika a molekulární biologie

Stát vydavatele

Kanada

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/03:00008100

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

MALDI; staphylococcal bacteriophage; proteome

Štítky

MALDI, proteome, staphylococcal bacteriophage

Změněno: 3. 3. 2006 14:34, prof. Mgr. Jan Preisler, Ph.D.

Anotace

V originále

The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. Genetic engineering has been previously applied for obtaining phage mutants with the desired host specificity. However, the genomic sequence similarity of the different phages is generally low, and identification of proteins with the same function in various phages based solely on the DNA sequence is often impossible. Furthermore, amino acid modifications, such as variants and post-translational modifications of the predicted proteins cannot be deduced from the DNA sequence. In this work, we propose new approaches to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis. Additionally, protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS.

Návaznosti

GA203/03/0515, projekt VaV

Název: Integrovaná analýza genomu a proteomu terapeuticky významných bakteriofágů kombinací elektroforézy a hmotnostní spektrometrie

Investor: Grantová agentura ČR, Integrovaná analýza genomu a proteomu terapeuticky významných bakteriofágů kombinací elektroforézy a hmotnostní spektrometrie

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

Citovat

PREISLER, Jan, Zbyněk ZDRÁHAL, Karel KLEPÁRNÍK, František FORET, Patrik VRÁBEL, Zdena MALÁ, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ, Hana KONEČNÁ a Pavel KRÁSENSKÝ. Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry. In Proceedings of the 51st Annual Conference on Mass Spectrometry and Allied Topics. Montreal: American Society for Mass Spectrometry, 2003, s. 1152.

@inproceedings{487584,
   author = {Preisler, Jan and Zdráhal, Zbyněk and Klepárník, Karel and Foret, František and Vrábel, Patrik and Malá, Zdena and Doškař, Jiří and Pantůček, Roman and Růžičková, Vladislava and Konečná, Hana and Krásenský, Pavel},
   address = {Montreal},
   booktitle = {Proceedings of the 51st Annual Conference on Mass Spectrometry and Allied Topics},
   keywords = {MALDI; staphylococcal bacteriophage; proteome},
   language = {eng},
   location = {Montreal},
   pages = {1152-1152},
   publisher = {American Society for Mass Spectrometry},
   title = {Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry},
   year = {2003}
}

TY  - CONF
ID  - 487584
AU  - Preisler, Jan - Zdráhal, Zbyněk - Klepárník, Karel - Foret, František - Vrábel, Patrik - Malá, Zdena - Doškař, Jiří - Pantůček, Roman - Růžičková, Vladislava - Konečná, Hana - Krásenský, Pavel
PY  - 2003
TI  - Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry
PB  - American Society for Mass Spectrometry
CY  - Montreal
KW  - MALDI
KW  - staphylococcal bacteriophage
KW  - proteome
N2  - The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. Genetic engineering has been previously applied for obtaining phage mutants with the desired host specificity. However, the genomic sequence similarity of the different phages is generally low, and identification of proteins with the same function in various phages based solely on the DNA sequence is often impossible. Furthermore, amino acid modifications, such as variants and post-translational modifications of the predicted proteins cannot be deduced from the DNA sequence. In this work, we propose new approaches to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis. Additionally, protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS.
ER  -

PREISLER, Jan, Zbyněk ZDRÁHAL, Karel KLEPÁRNÍK, František FORET, Patrik VRÁBEL, Zdena MALÁ, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ, Hana KONEČNÁ a Pavel KRÁSENSKÝ. Proteomic Analysis of Therapeutically Important Bacteriophage 812 by Combination of Electrophoresis and Mass Spectrometry. In \textit{Proceedings of the 51st Annual Conference on Mass Spectrometry and Allied Topics}. Montreal: American Society for Mass Spectrometry, 2003, s.~1152.

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