Electrochemical determination of d(GCGAAAGC) hairpin

D 2003

Electrochemical determination of d(GCGAAAGC) hairpin

TRNKOVÁ, Libuše; Irena POSTBIEGLOVÁ a Miroslav HOLÍK

Základní údaje

Originální název

Electrochemical determination of d(GCGAAAGC) hairpin

Autoři

TRNKOVÁ, Libuše; Irena POSTBIEGLOVÁ a Miroslav HOLÍK

Vydání

1. vyd. Florence, Italy, XVII th International Symposium on Bioelectrochemistry and Bioenergetics, s. 138-138, 2003

Nakladatel

The Bioelectrochemical Society

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10405 Electrochemistry

Stát vydavatele

Itálie

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/03:00008111

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

hairpin; linear sweep and cyclic voltammetry; elimination voltammetry with linear scan; Fourier Transform;confidence ellipse

Štítky

confidence ellipse, Fourier Transform, hairpin

Změněno: 20. 6. 2008 12:03, prof. RNDr. Libuše Trnková, CSc.

Anotace

V originále

Hairpins (or hairpin-like structures) may play a major role in expansion events of triplet repeat expansion diseases (X syndrome, Huntington s disease, Friedeich s ataxia). The d(GCGAAGC) fragment has been found in the replication origins of phage fX 174 and herpes simplex virus, in a promoter region of an E.coli heat-shock gene, and in rRNA genes. Recently, the structure of d(GCGAAGC) has been determined by NMR spectroscopy and refined using molecular dynamics [1]. For fast and economical determination of the hairpin in biological materials applicability of voltammetric methods was investigated. On mercury electrodes the d(GCGAAGC) hairpin provides a voltammetric reduction signal (A and C) and an oxidation signal (G). Both signals have been studied by cyclic voltammetry and square wave voltammetry in dependence on pH change, accumulation time, scan rate, and exchange of minihairpin sequences. The elimination voltammetry with linear scan (EVLS) [2-4] in combination with the adsorptive stripping the A and C reduction peak in the d(GCGXAGC) hairpin (X = A,C,T,G) has been employed to the determination of detection limits (~ nM). Multidimensional data of CV and EVLS were worked up by Fourier Transform (FT) and for the first coefficient a confidence ellipse was calculated in order to drop out some outlier data. The same method was used for detection limit determinations.

Návaznosti

GA203/02/0422, projekt VaV

Název: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

Investor: Grantová agentura ČR, Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

IAA1163201, projekt VaV

Název: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Investor: Akademie věd ČR, Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Citovat

TRNKOVÁ, Libuše; Irena POSTBIEGLOVÁ a Miroslav HOLÍK. Electrochemical determination of d(GCGAAAGC) hairpin. In XVII th International Symposium on Bioelectrochemistry and Bioenergetics. 1. vyd. Florence, Italy: The Bioelectrochemical Society, 2003, s. 138.

@inproceedings{487723,
   author = {Trnková, Libuše and Postbieglová, Irena and Holík, Miroslav},
   address = {Florence, Italy},
   booktitle = {XVII th International Symposium on Bioelectrochemistry and Bioenergetics},
   edition = {1},
   keywords = {hairpin; linear sweep and cyclic voltammetry; elimination voltammetry with linear scan; Fourier Transform;confidence ellipse},
   language = {eng},
   location = {Florence, Italy},
   pages = {138-138},
   publisher = {The Bioelectrochemical Society},
   title = {Electrochemical determination of d(GCGAAAGC) hairpin},
   year = {2003}
}

TY  - CONF
ID  - 487723
AU  - Trnková, Libuše - Postbieglová, Irena - Holík, Miroslav
PY  - 2003
TI  - Electrochemical determination of d(GCGAAAGC) hairpin
PB  - The Bioelectrochemical Society
CY  - Florence, Italy
KW  - hairpin
KW  - linear sweep and cyclic voltammetry
KW  - elimination voltammetry with linear scan
KW  - Fourier Transform;confidence ellipse
N2  - Hairpins (or hairpin-like structures) may play a major role in expansion events of triplet repeat expansion diseases (X syndrome, Huntington s disease, Friedeich s ataxia). The d(GCGAAGC) fragment has been found in the replication origins of phage fX 174 and herpes simplex virus, in a promoter region of an E.coli heat-shock gene, and in rRNA genes. Recently, the structure of d(GCGAAGC) has been determined by NMR spectroscopy and refined using molecular dynamics [1]. For fast and economical determination of the hairpin in biological materials applicability of voltammetric methods was investigated. On mercury electrodes the d(GCGAAGC) hairpin provides a voltammetric reduction signal (A and C) and an oxidation signal (G). Both signals have been studied by cyclic voltammetry and square wave voltammetry in dependence on pH change, accumulation time, scan rate, and exchange of minihairpin sequences. The elimination voltammetry with linear scan (EVLS) [2-4] in combination with the adsorptive stripping the A and C reduction peak in the d(GCGXAGC) hairpin (X = A,C,T,G) has been employed to the determination of detection limits (~ nM). Multidimensional data of CV and EVLS were worked up by Fourier Transform (FT) and for the first coefficient a confidence ellipse was calculated in order to drop out some outlier data. The same method was used for detection limit determinations.
ER  -

TRNKOVÁ, Libuše; Irena POSTBIEGLOVÁ a Miroslav HOLÍK. Electrochemical determination of d(GCGAAAGC) hairpin. In \textit{XVII th International Symposium on Bioelectrochemistry and Bioenergetics}. 1. vyd. Florence, Italy: The Bioelectrochemical Society, 2003, s.~138.

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