Retron reverse transcriptase rrtT is ubiquitous in strains of
Salmonella enterica serovar Typhimurium

J 2003

Retron reverse transcriptase rrtT is ubiquitous in strains of Salmonella enterica serovar Typhimurium

MATIASOVIČOVÁ, Jitka; Marcela FALDYNOVÁ; Martina PRAVCOVÁ; Renata KARPÍŠKOVÁ; Ivana KOLÁČKOVÁ et al.

Základní údaje

Originální název

Retron reverse transcriptase rrtT is ubiquitous in strains of Salmonella enterica serovar Typhimurium

Autoři

MATIASOVIČOVÁ, Jitka; Marcela FALDYNOVÁ; Martina PRAVCOVÁ; Renata KARPÍŠKOVÁ; Ivana KOLÁČKOVÁ; Jiří DAMBORSKÝ a Ivan RYCHLÍK

Vydání

FEMS Microbiology Letters, 2003, 0378-1097

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.932

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/03:00008817

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000183964700021

Klíčová slova anglicky

RRTT; TYPHYMURIUM; SALMONELLA; ENTERICA

Štítky

ENTERICA, RRTT, Salmonella, TYPHYMURIUM

Změněno: 19. 3. 2010 11:05, prof. Mgr. Jiří Damborský, Dr.

Anotace

V originále

Bacterial retron reverse transcriptases are unusual enzymes which utilise the same RNA molecule as a template and also as a primer for initiation of the reverse transcription. Except for their relatively frequent presence in Myxococcus spp., they are considered as quite rare proteins. However, in this study we proved that retron reverse transcriptase is frequently found in certain serovars of Salmonella enterica. Using PCR, in strains of serovar Typhimurium, the rrtT (retron reverse transcriptase Typhimurium) gene was detected in 158 out of 175 tested field strains. On the other hand, in none of the 18 tested serovar Enteritidis strains the rrtT was detected in their genome. Detailed computer analysis allowed us to predict the sequence of msDNA and to propose that the final msDNA is free of any RNA. Furthermore, we predict that there are at least three different classes of retron reverse transcriptases.

Návaznosti

LN00A016, projekt VaV

Název: BIOMOLEKULÁRNÍ CENTRUM

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

Citovat

MATIASOVIČOVÁ, Jitka; Marcela FALDYNOVÁ; Martina PRAVCOVÁ; Renata KARPÍŠKOVÁ; Ivana KOLÁČKOVÁ; Jiří DAMBORSKÝ a Ivan RYCHLÍK. Retron reverse transcriptase rrtT is ubiquitous in strains of Salmonella enterica serovar Typhimurium. FEMS Microbiology Letters. 2003, roč. 223, č. 2, s. 281-286. ISSN 0378-1097.

@article{487898,
   author = {Matiasovičová, Jitka and Faldynová, Marcela and Pravcová, Martina and Karpíšková, Renata and Koláčková, Ivana and Damborský, Jiří and Rychlík, Ivan},
   article_number = {2},
   keywords = {RRTT; TYPHYMURIUM; SALMONELLA; ENTERICA},
   language = {eng},
   issn = {0378-1097},
   journal = {FEMS Microbiology Letters},
   title = {Retron reverse transcriptase rrtT is ubiquitous in strains of Salmonella enterica serovar Typhimurium},
   url = {http://ncbr.chemi.muni.cz/~jiri/abstracts/femslett03.html},
   volume = {223},
   year = {2003}
}

TY  - JOUR
ID  - 487898
AU  - Matiasovičová, Jitka - Faldynová, Marcela - Pravcová, Martina - Karpíšková, Renata - Koláčková, Ivana - Damborský, Jiří - Rychlík, Ivan
PY  - 2003
TI  - Retron reverse transcriptase rrtT is ubiquitous in strains of Salmonella enterica serovar Typhimurium
JF  - FEMS Microbiology Letters
VL  - 223
IS  - 2
SP  - 281-286
EP  - 281-286
SN  - 03781097
KW  - RRTT
KW  - TYPHYMURIUM
KW  - SALMONELLA
KW  - ENTERICA
UR  - http://ncbr.chemi.muni.cz/~jiri/abstracts/femslett03.html
N2  - Bacterial retron reverse transcriptases are unusual enzymes which utilise the same RNA molecule as a template and also as a primer for initiation of the reverse transcription. Except for their relatively frequent presence in Myxococcus spp., they are considered as quite rare proteins. However, in this study we proved that retron reverse transcriptase is frequently found in certain serovars of Salmonella enterica. Using PCR, in strains of serovar Typhimurium, the rrtT (retron reverse transcriptase Typhimurium) gene was detected in 158 out of 175 tested field strains. On the other hand, in none of the 18 tested serovar Enteritidis strains the rrtT was detected in their genome. Detailed computer analysis allowed us to predict the sequence of msDNA and to propose that the final msDNA is free of any RNA. Furthermore, we predict that there are at least three different classes of retron reverse transcriptases.
ER  -

MATIASOVIČOVÁ, Jitka; Marcela FALDYNOVÁ; Martina PRAVCOVÁ; Renata KARPÍŠKOVÁ; Ivana KOLÁČKOVÁ; Jiří DAMBORSKÝ a Ivan RYCHLÍK. Retron reverse transcriptase rrtT is ubiquitous in strains of Salmonella enterica serovar Typhimurium. \textit{FEMS Microbiology Letters}. 2003, roč.~223, č.~2, s.~281-286. ISSN~0378-1097.

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