Molecular and Physiological Characterisation of an Insertion
Mutant in the ARR21 Putative Response Regulator Gene from
Arabidopsis thaliana

J 2003

Molecular and Physiological Characterisation of an Insertion Mutant in the ARR21 Putative Response Regulator Gene from Arabidopsis thaliana

HORÁK, Jakub; Břetislav BRZOBOHATÝ a Matej LEXA

Základní údaje

Originální název

Molecular and Physiological Characterisation of an Insertion Mutant in the ARR21 Putative Response Regulator Gene from Arabidopsis thaliana

Autoři

HORÁK, Jakub; Břetislav BRZOBOHATÝ a Matej LEXA

Vydání

Plant Biology, Stuttgart, Thieme, Georg Thieme Verlag, 2003, 1435-8603

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.420

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/03:00008134

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000184511500003

Klíčová slova anglicky

SLAT - insertion lines - two-component system - response regulator - signal transduction - Arabidopsis thaliana

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 6. 2009 14:05, Mgr. Jakub Horák, Dr. rer. nat.

Anotace

V originále

In our search for insertion mutants of Arabidopsis response regulator (ARR) genes, we identified a candidate for an ARR21 dSpm transposon insertion line in the SLAT collection by searching the SINS sequence database. Molecular characterisation of this line revealed that the transposon is integrated as a single copy 1727 bases downstream of the ATG signal, within the third intron of the ARR21 gene. The transposon insertion segregated in a Mendelian fashion from heterozygous plants that were allowed to self-pollinate. RT-PCR-based expression analysis showed that ARR21 transcript predominantly accumulates in siliques. In contrast, the full-length ARR21 transcript was not detectable in the ARR21 transposon insertion line, indicating that it harbours an insertion of dSpm transposon in the ARR21 gene. The ARR21 insertion mutant (arr21-1) was subjected to several physiological tests for a possible insertion-linked phenotype. However, our results revealed that the insertion in the ARR21 gene did not cause any alterations in viability and fertility, flowering time, sensitivity to ethylene, cytokinin or red light. We discuss these results in the light of recent findings about the function of the other members of the response regulator gene family of Arabidopsis.

Návaznosti

GA204/99/D024, projekt VaV

Název: Funkce homologů bakteriálních regulátorů indukovatelných dusičnany v rostlinách Arabidopsis thaliana

Investor: Grantová agentura ČR, Funkce homologů bakteriálních regulátorů indukovatelných dusičnany v rostlinách Arabidopsis thaliana

LN00A081, projekt VaV

Název: Signální dráhy u rostlin

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

Citovat

HORÁK, Jakub; Břetislav BRZOBOHATÝ a Matej LEXA. Molecular and Physiological Characterisation of an Insertion Mutant in the ARR21 Putative Response Regulator Gene from Arabidopsis thaliana. Plant Biology. Stuttgart: Thieme, Georg Thieme Verlag, 2003, roč. 5, č. 3, s. 249-254, 10 s. ISSN 1435-8603.

@article{488140,
   author = {Horák, Jakub and Brzobohatý, Břetislav and Lexa, Matej},
   article_location = {Stuttgart},
   article_number = {3},
   keywords = {SLAT - insertion lines - two-component system - response regulator - signal transduction - Arabidopsis thaliana},
   language = {eng},
   issn = {1435-8603},
   journal = {Plant Biology},
   title = {Molecular and Physiological Characterisation of an Insertion Mutant in the ARR21 Putative Response Regulator Gene from Arabidopsis thaliana},
   volume = {5},
   year = {2003}
}

TY  - JOUR
ID  - 488140
AU  - Horák, Jakub - Brzobohatý, Břetislav - Lexa, Matej
PY  - 2003
TI  - Molecular and Physiological Characterisation of an Insertion Mutant in the ARR21 Putative Response Regulator Gene from Arabidopsis thaliana
JF  - Plant Biology
VL  - 5
IS  - 3
SP  - 249-254
EP  - 249-254
PB  - Thieme, Georg Thieme Verlag
SN  - 14358603
KW  - SLAT - insertion lines - two-component system - response regulator - signal transduction - Arabidopsis thaliana
N2  - In our search for insertion mutants of Arabidopsis response regulator (ARR) genes, we identified a candidate for an ARR21 dSpm transposon insertion line in the SLAT collection by searching the SINS sequence database. Molecular characterisation of this line revealed that the transposon is integrated as a single copy 1727 bases downstream of the ATG signal, within the third intron of the ARR21 gene. The transposon insertion segregated in a Mendelian fashion from heterozygous plants that were allowed to self-pollinate. RT-PCR-based expression analysis showed that ARR21 transcript predominantly accumulates in siliques. In contrast, the full-length ARR21 transcript was not detectable in the ARR21 transposon insertion line, indicating that it harbours an insertion of dSpm transposon in the ARR21 gene. The ARR21 insertion mutant (arr21-1) was subjected to several physiological tests for a possible insertion-linked phenotype. However, our results revealed that the insertion in the ARR21 gene did not cause any alterations in viability and fertility, flowering time, sensitivity to ethylene, cytokinin or red light. We discuss these results in the light of recent findings about the function of the other members of the response regulator gene family of Arabidopsis.
ER  -

HORÁK, Jakub; Břetislav BRZOBOHATÝ a Matej LEXA. Molecular and Physiological Characterisation of an Insertion Mutant in the ARR21 Putative Response Regulator Gene from Arabidopsis thaliana. \textit{Plant Biology}. Stuttgart: Thieme, Georg Thieme Verlag, 2003, roč.~5, č.~3, s.~249-254, 10 s. ISSN~1435-8603.

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