Structural and thermodynamical characterisation  of interaction
between a pseudomonas lectin and monosaccharides

D 2003

Structural and thermodynamical characterisation of interaction between a pseudomonas lectin and monosaccharides

SABIN, Charles, Michaela WIMMEROVÁ, Martina BUDOVÁ, Lenka ŠNAJDROVÁ, Catherine GAUTIER et. al.

Základní údaje

Originální název

Structural and thermodynamical characterisation of interaction between a pseudomonas lectin and monosaccharides

Autoři

SABIN, Charles (250 Francie), Michaela WIMMEROVÁ (203 Česká republika, garant), Martina BUDOVÁ (250 Francie), Lenka ŠNAJDROVÁ (250 Francie), Catherine GAUTIER (250 Francie), Jaroslav KOČA (376 Izrael), Edward MITCHELL (250 Francie), Nechama GILBOA-GARBER (376 Izrael) a Anne IMBERTY (250 Francie)

Vydání

Grenoble, France, 12th European Carbohydrate Symposium, s. 390-390, 2003

Nakladatel

CERMAV-CNRS

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/03:00008865

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Pseudomonas aeruginosa; lectin; microcalorimetry; crystal structure; cystic fibrosis

Štítky

Crystal Structure, cystic fibrosis, lectin, microcalorimetry, Pseudomonas aeruginosa

Příznaky

Mezinárodní význam

Změněno: 15. 11. 2006 11:39, prof. RNDr. Michaela Wimmerová, Ph.D.

Anotace

V originále

Pseudomonas aeruginosa galactose- and fucose-binding (PA-IL and PA-IIL) lectins contribute to the virulence of this pathogenic bacterium, which is a major cause of morbidity and mortality in cystic fibrosis patients. The crystal structure of PA-IIL complexed with fucose has been solved at 1.3 A and further refined at 1 Ĺ resolution. Additional experiments have been performed in order to understand the molecular basis of both specificity and affinity of PA-IIL for monosaccharides. Crystals have been obtained for the complex between PA-ILL and b-Me-D-arabinopyranoside and the structure has been solved at 1.8 A resolution. PA-IIL exhibits unusually high specificity to fucose, which can be related to the presence of two calcium ions in the site. Isothermal titration microcalorimetry (ITC) experiments were performed in order to characterize the thermodynamic parameters of the interaction since this method has been proven to be well adapted to the study of protein/carbohydrate interactions. Several monosaccharides differing only by the group at C5 (L-fucose, L-galactose and D-arabinose) were tested, as well as their methyl-glycosides. Calculations of charges distribution were performed using quantum chemistry in order to rationalize the role of calcium ions in the affinity.

Návaznosti

LN00A016, projekt VaV

Název: BIOMOLEKULÁRNÍ CENTRUM

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

Citovat

SABIN, Charles, Michaela WIMMEROVÁ, Martina BUDOVÁ, Lenka ŠNAJDROVÁ, Catherine GAUTIER, Jaroslav KOČA, Edward MITCHELL, Nechama GILBOA-GARBER a Anne IMBERTY. Structural and thermodynamical characterisation of interaction between a pseudomonas lectin and monosaccharides. In 12th European Carbohydrate Symposium. Grenoble, France: CERMAV-CNRS, 2003, s. 390.

@inproceedings{488303,
   author = {Sabin, Charles and Wimmerová, Michaela and Budová, Martina and Šnajdrová, Lenka and Gautier, Catherine and Koča, Jaroslav and Mitchell, Edward and GilboaandGarber, Nechama and Imberty, Anne},
   address = {Grenoble, France},
   booktitle = {12th European Carbohydrate Symposium},
   keywords = {Pseudomonas aeruginosa; lectin; microcalorimetry; crystal structure; cystic fibrosis},
   language = {eng},
   location = {Grenoble, France},
   pages = {390-390},
   publisher = {CERMAV-CNRS},
   title = {Structural and thermodynamical characterisation of interaction between a pseudomonas lectin and monosaccharides},
   year = {2003}
}

TY  - JOUR
ID  - 488303
AU  - Sabin, Charles - Wimmerová, Michaela - Budová, Martina - Šnajdrová, Lenka - Gautier, Catherine - Koča, Jaroslav - Mitchell, Edward - Gilboa-Garber, Nechama - Imberty, Anne
PY  - 2003
TI  - Structural and thermodynamical characterisation of interaction between a pseudomonas lectin and monosaccharides
PB  - CERMAV-CNRS
CY  - Grenoble, France
KW  - Pseudomonas aeruginosa
KW  - lectin
KW  - microcalorimetry
KW  - crystal structure
KW  - cystic fibrosis
N2  - Pseudomonas aeruginosa galactose- and fucose-binding (PA-IL and PA-IIL) lectins contribute to the virulence of this pathogenic bacterium, which is a major cause of morbidity and mortality in cystic fibrosis patients. The crystal structure of PA-IIL complexed with fucose has been solved at 1.3 A and further refined at 1 Ĺ resolution. Additional experiments have been performed in order to understand the molecular basis of both specificity and affinity of PA-IIL for monosaccharides. Crystals have been obtained for the complex between PA-ILL and b-Me-D-arabinopyranoside and the structure has been solved at 1.8 A resolution. PA-IIL exhibits unusually high specificity to fucose, which can be related to the presence of two calcium ions in the site. Isothermal titration microcalorimetry (ITC) experiments were performed in order to characterize the thermodynamic parameters of the interaction since this method has been proven to be well adapted to the study of protein/carbohydrate interactions. Several monosaccharides differing only by the group at C5 (L-fucose, L-galactose and D-arabinose) were tested, as well as their methyl-glycosides. Calculations of charges distribution were performed using quantum chemistry in order to rationalize the role of calcium ions in the affinity.
ER  -

SABIN, Charles, Michaela WIMMEROVÁ, Martina BUDOVÁ, Lenka ŠNAJDROVÁ, Catherine GAUTIER, Jaroslav KOČA, Edward MITCHELL, Nechama GILBOA-GARBER a Anne IMBERTY. Structural and thermodynamical characterisation of interaction between a pseudomonas lectin and monosaccharides. In \textit{12th European Carbohydrate Symposium}. Grenoble, France: CERMAV-CNRS, 2003, s.~390.

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