Structural basis for oligosaccharide-mediated adhesion of P.
aeruginosa in the lungs of cystic fibrosis patients

D 2003

Structural basis for oligosaccharide-mediated adhesion of P. aeruginosa in the lungs of cystic fibrosis patients

MITCHELL, Edward, Corrine HOULES, Charles SABIN, Michaela WIMMEROVÁ, Catherine GAUTIER et. al.

Základní údaje

Originální název

Structural basis for oligosaccharide-mediated adhesion of P. aeruginosa in the lungs of cystic fibrosis patients

Autoři

MITCHELL, Edward (826 Velká Británie a Severní Irsko), Corrine HOULES (250 Francie), Charles SABIN (250 Francie), Michaela WIMMEROVÁ (203 Česká republika, garant), Catherine GAUTIER (250 Francie), Serge PEREZ (250 Francie), Albert M. WU (158 Tchaj-wan), Nechama GILBOA-GARBER (376 Izrael) a Anne IMBERTY (250 Francie)

Vydání

Grenoble, France, 12th European Carbohydrate Symposium, s. 61-61, 2003

Nakladatel

CERMAV-CNRS

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/03:00008868

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Pseudomonas aeruginosa; lectin; microcalorimetry; crystal structure; cystic fibrosis

Štítky

Crystal Structure, cystic fibrosis, lectin, microcalorimetry, Pseudomonas aeruginosa

Příznaky

Mezinárodní význam

Změněno: 15. 11. 2006 11:39, prof. RNDr. Michaela Wimmerová, Ph.D.

Anotace

V originále

The galactose- and fucose-binding (PA-IL and PA-IIL) lectins of Pseudomonas aeruginosa contribute to the virulence of this pathogenic bacterium, which is a major cause of morbidity and mortality in cystic fibrosis (CF) patients via chronic lung colonisation. CF gene mutations increase cell surface fucosylation and CF patients also display modifications in their respiratory and salivary mucins with a higher percentage of sialylated and sulphated oligosaccharides. These cystic fibrosis mucins and cell surface glycoconjugates carry fucose as the terminal sugar residue. Since the P. aeruginosa lectins have been characterised to reveal an outstandingly high affinity of PA-IIL for fucose, they can serve as binding targets for binding by PA-IIL [1]. Precise three-dimensional knowledge of the lectin sugar binding site, through protein crystallography at high resolution, has allowed the unusually high affinity to be understood and shown a novel sugar binding mode. Subsequent modelling studies, based on the fucose complex structure, and binding studies have demonstrated that the preferred ligands of this bacterial lectin belong to the Lea series. Such structure-based knowledge could be used for the design of efficient anti-bacterial compounds and, furthermore, the unusually high affinity interaction of this novel binding mode suggests that PA-IIL may be a useful target for oligosaccharide-based therapeutics.

Návaznosti

LN00A016, projekt VaV

Název: BIOMOLEKULÁRNÍ CENTRUM

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

Citovat

MITCHELL, Edward, Corrine HOULES, Charles SABIN, Michaela WIMMEROVÁ, Catherine GAUTIER, Serge PEREZ, Albert M. WU, Nechama GILBOA-GARBER a Anne IMBERTY. Structural basis for oligosaccharide-mediated adhesion of P. aeruginosa in the lungs of cystic fibrosis patients. In 12th European Carbohydrate Symposium. Grenoble, France: CERMAV-CNRS, 2003, s. 61.

@inproceedings{488307,
   author = {Mitchell, Edward and Houles, Corrine and Sabin, Charles and Wimmerová, Michaela and Gautier, Catherine and Perez, Serge and Wu, Albert M. and GilboaandGarber, Nechama and Imberty, Anne},
   address = {Grenoble, France},
   booktitle = {12th European Carbohydrate Symposium},
   keywords = {Pseudomonas aeruginosa; lectin; microcalorimetry; crystal structure; cystic fibrosis},
   language = {eng},
   location = {Grenoble, France},
   pages = {61-61},
   publisher = {CERMAV-CNRS},
   title = {Structural basis for oligosaccharide-mediated adhesion of P. aeruginosa in the lungs of cystic fibrosis patients},
   year = {2003}
}

TY  - JOUR
ID  - 488307
AU  - Mitchell, Edward - Houles, Corrine - Sabin, Charles - Wimmerová, Michaela - Gautier, Catherine - Perez, Serge - Wu, Albert M. - Gilboa-Garber, Nechama - Imberty, Anne
PY  - 2003
TI  - Structural basis for oligosaccharide-mediated adhesion of P. aeruginosa in the lungs of cystic fibrosis patients
PB  - CERMAV-CNRS
CY  - Grenoble, France
KW  - Pseudomonas aeruginosa
KW  - lectin
KW  - microcalorimetry
KW  - crystal structure
KW  - cystic fibrosis
N2  - The galactose- and fucose-binding (PA-IL and PA-IIL) lectins of Pseudomonas aeruginosa contribute to the virulence of this pathogenic bacterium, which is a major cause of morbidity and mortality in cystic fibrosis (CF) patients via chronic lung colonisation. CF gene mutations increase cell surface fucosylation and CF patients also display modifications in their respiratory and salivary mucins with a higher percentage of sialylated and sulphated oligosaccharides. These cystic fibrosis mucins and cell surface glycoconjugates carry fucose as the terminal sugar residue. Since the P. aeruginosa lectins have been characterised to reveal an outstandingly high affinity of PA-IIL for fucose, they can serve as binding targets for binding by PA-IIL [1]. Precise three-dimensional knowledge of the lectin sugar binding site, through protein crystallography at high resolution, has allowed the unusually high affinity to be understood and shown a novel sugar binding mode. Subsequent modelling studies, based on the fucose complex structure, and binding studies have demonstrated that the preferred ligands of this bacterial lectin belong to the Lea series. Such structure-based knowledge could be used for the design of efficient anti-bacterial compounds and, furthermore, the unusually high affinity interaction of this novel binding mode suggests that PA-IIL may be a useful target for oligosaccharide-based therapeutics.
ER  -

MITCHELL, Edward, Corrine HOULES, Charles SABIN, Michaela WIMMEROVÁ, Catherine GAUTIER, Serge PEREZ, Albert M. WU, Nechama GILBOA-GARBER a Anne IMBERTY. Structural basis for oligosaccharide-mediated adhesion of P. aeruginosa in the lungs of cystic fibrosis patients. In \textit{12th European Carbohydrate Symposium}. Grenoble, France: CERMAV-CNRS, 2003, s.~61.

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