Changes of immunofluorescence staining for ECM molecules in the
satellite cells of rat dorsal root ganglia following a chronic
constriction injury of the peripheral nerve

J 2003

Changes of immunofluorescence staining for ECM molecules in the satellite cells of rat dorsal root ganglia following a chronic constriction injury of the peripheral nerve

DUBOVÝ, Petr, Radim JANČÁLEK a Ilona KLUSÁKOVÁ

Základní údaje

Originální název

Changes of immunofluorescence staining for ECM molecules in the satellite cells of rat dorsal root ganglia following a chronic constriction injury of the peripheral nerve

Autoři

DUBOVÝ, Petr (203 Česká republika, garant), Radim JANČÁLEK (203 Česká republika) a Ilona KLUSÁKOVÁ (203 Česká republika)

Vydání

Glia Suppl, USA, Wiley-Liss, Inc. 2003, 0894-1491

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.677

Kód RIV

RIV/00216224:14110/03:00008386

Organizační jednotka

Lékařská fakulta

UT WoS

000184938300154

Klíčová slova anglicky

laminin; chondroitin sulfate; tenascin; fibronectin; endoneurium

Štítky

chondroitin sulfate, endoneurium, fibronectin, laminin, tenascin

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 6. 2009 10:49, prof. RNDr. Petr Dubový, CSc.

Anotace

ORIG CZ

V originále

We have investigated immunostaining for some ECM molecules ( chondroitin sulfate, tenascin, thrombospondin, fibronectin) in the DRG satellite cells following CCI of the peripheral nerve. The experimental CCI was applied ipsilaterally on the spinal nerve and the sciatic nerve of Wistar rats (12 females, 280g). Both ipsilateral and contralateral DRG (L4-L5) were removed 1, 2, 3, 5 weeks after the operation. The immunofluorescence staining for ECM molecules was compared in the sections through ipsilateral and contralateral DRG by an image analysis system. The satellite cell sheaths of ipsilateral DRG (L4-L5) displayed a higher brightness of immunofluorescence staining for investigated ECM molecules in comparison to the contralateral ones. The changes were more noticeable after CCI applied near to DRG (4mm) than in longer distance (40mm). An increased immunostaining for the ECM molecules in the satellite cells following CCI of peripheral nerve suggested that the changes may be implicated in growth stimuli for sympathetic sprouts and formation of sympathetic baskets.

Česky

Molekuly ECM (CSPG, tenascin, thrombospondin, fibronectin) byly imunohistochemicky detekovány ve spinálních gangliích po chronické konstrikci nervu.

Návaznosti

GA309/00/0407, projekt VaV

Název: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Grantová agentura ČR, Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Citovat

DUBOVÝ, Petr, Radim JANČÁLEK a Ilona KLUSÁKOVÁ. Changes of immunofluorescence staining for ECM molecules in the satellite cells of rat dorsal root ganglia following a chronic constriction injury of the peripheral nerve. Glia Suppl. USA: Wiley-Liss, Inc., 2003, roč. 2, č. 1, s. 36-37. ISSN 0894-1491.

@article{490969,
   author = {Dubový, Petr and Jančálek, Radim and Klusáková, Ilona},
   article_location = {USA},
   article_number = {1},
   keywords = {laminin; chondroitin sulfate; tenascin; fibronectin; endoneurium},
   language = {eng},
   issn = {0894-1491},
   journal = {Glia Suppl},
   title = {Changes of immunofluorescence staining for ECM molecules in the satellite cells of rat dorsal root ganglia following a chronic constriction injury of the peripheral nerve},
   volume = {2},
   year = {2003}
}

TY  - JOUR
ID  - 490969
AU  - Dubový, Petr - Jančálek, Radim - Klusáková, Ilona
PY  - 2003
TI  - Changes of immunofluorescence staining for ECM molecules in the satellite cells of rat dorsal root ganglia following a chronic constriction injury of the peripheral nerve
JF  - Glia Suppl
VL  - 2
IS  - 1
SP  - 36
EP  - 36
PB  - Wiley-Liss, Inc.
SN  - 08941491
KW  - laminin
KW  - chondroitin sulfate
KW  - tenascin
KW  - fibronectin
KW  - endoneurium
N2  - We have investigated immunostaining for some ECM molecules ( chondroitin sulfate, tenascin, thrombospondin, fibronectin) in the DRG satellite cells following CCI of the peripheral nerve. The experimental CCI was applied ipsilaterally on the spinal nerve and the sciatic nerve of Wistar rats (12 females, 280g). Both ipsilateral and contralateral DRG (L4-L5) were removed 1, 2, 3, 5 weeks after the operation. The immunofluorescence staining for ECM molecules was compared in the sections through ipsilateral and contralateral DRG by an image analysis system. The satellite cell sheaths of ipsilateral DRG (L4-L5) displayed a higher brightness of immunofluorescence staining for investigated ECM molecules in comparison to the contralateral ones. The changes were more noticeable after CCI applied near to DRG (4mm) than in longer distance (40mm). An increased immunostaining for the ECM molecules in the satellite cells following CCI of peripheral nerve suggested that the changes may be implicated in growth stimuli for sympathetic sprouts and formation of sympathetic baskets.
ER  -

DUBOVÝ, Petr, Radim JANČÁLEK a Ilona KLUSÁKOVÁ. Changes of immunofluorescence staining for ECM molecules in the satellite cells of rat dorsal root ganglia following a chronic constriction injury of the peripheral nerve. \textit{Glia Suppl}. USA: Wiley-Liss, Inc., 2003, roč.~2, č.~1, s.~36-37. ISSN~0894-1491.

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