CAPILLARY ELECTROPHORESIS AS A TOOL FOR THE STUDY OF ENZYMES

D 2003

CAPILLARY ELECTROPHORESIS AS A TOOL FOR THE STUDY OF ENZYMES

VYTISKOVÁ, Soňa a Zdeněk GLATZ

Základní údaje

Originální název

CAPILLARY ELECTROPHORESIS AS A TOOL FOR THE STUDY OF ENZYMES

Autoři

VYTISKOVÁ, Soňa (203 Česká republika) a Zdeněk GLATZ (203 Česká republika, garant)

Vydání

Bratislava, Proceeding of 15th Internationa Conference "Chromatographic Methods and Human Health" od s. 30-30, 1 s. 2003

Nakladatel

Slovenská chemická společnost

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/03:00008402

Organizační jednotka

Přírodovědecká fakulta

ISSN

Klíčová slova anglicky

Capillary electrophoresis Enzymes EMMA

Štítky

Capillary electrophoresis Enzymes EMMA

Změněno: 19. 5. 2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.

Anotace

V originále

Enzymes are biological catalysts that play an important role in biochemical reactions necessary for normal growth, maturation and reproduction through whole live world. Due to their low concentrations in the samples containing large amount of other proteins, direct measurements of enzymes by masses are impossible. However enzymes can be measured more easily by their catalytic activities, which are the most relevant properties of enzymes in the biochemical context. The accurate measurement of enzymatic activity in biological samples is important in many fields of biochemistry, not only in routine biochemistry and in fundamental research but also in clinical and pharmacological research and diagnosis. The enzyme assay is also important procedure in elucidation of enzyme properties and function. Determination of kinetic parameters is usually undertaken to characterize an enzyme, to provide a quantitative evaluation of substrate specificity and to study kinetic mechanisms. In the last decade capillary electromigration separation techniques have become powerful techniques, which can provide highly efficient separations and large peak capacities. Today the availability of sophisticated automated equipment for CE makes the technique suitable to be implement in routine laboratories for separation of a variety of molecules including metal ions, organic and inorganic ions, drugs, peptides, proteins and DNA fragments, even whole bacterial cells and viruses. All these parameters predict CE as one of the method of choice also for determination of enzyme activities.

Návaznosti

GA203/03/1125, projekt VaV

Název: Využití kapilární zónové elektroforézy pro studium enzymů

Investor: Grantová agentura ČR, Využití kapilární zónové elektroforézy pro studium enzymů

Citovat

VYTISKOVÁ, Soňa a Zdeněk GLATZ. CAPILLARY ELECTROPHORESIS AS A TOOL FOR THE STUDY OF ENZYMES. In Proceeding of 15th Internationa Conference "Chromatographic Methods and Human Health". Bratislava: Slovenská chemická společnost, 2003, s. 30-30. ISSN 1335-5236.

@inproceedings{491152,
   author = {Vytisková, Soňa and Glatz, Zdeněk},
   address = {Bratislava},
   booktitle = {Proceeding of 15th Internationa Conference "Chromatographic Methods and Human Health"},
   keywords = {Capillary electrophoresis Enzymes EMMA},
   language = {eng},
   location = {Bratislava},
   pages = {30-30},
   publisher = {Slovenská chemická společnost},
   title = {CAPILLARY ELECTROPHORESIS AS A TOOL FOR THE STUDY OF ENZYMES},
   year = {2003}
}

TY  - JOUR
ID  - 491152
AU  - Vytisková, Soňa - Glatz, Zdeněk
PY  - 2003
TI  - CAPILLARY ELECTROPHORESIS AS A TOOL FOR THE STUDY OF ENZYMES
PB  - Slovenská chemická společnost
CY  - Bratislava
KW  - Capillary electrophoresis Enzymes EMMA
N2  - Enzymes are biological catalysts that play an important role in biochemical reactions necessary for normal growth, maturation and reproduction through whole live world. Due to their low concentrations in the samples containing large amount of other proteins, direct measurements of enzymes by masses are impossible. However enzymes can be measured more easily by their catalytic activities, which are the most relevant properties of enzymes in the biochemical context. The accurate measurement of enzymatic activity in biological samples is important in many fields of biochemistry, not only in routine biochemistry and in fundamental research but also in clinical and pharmacological research and diagnosis. The enzyme assay is also important procedure in elucidation of enzyme properties and function. Determination of kinetic parameters is usually undertaken to characterize an enzyme, to provide a quantitative evaluation of substrate specificity and to study kinetic mechanisms. In the last decade capillary electromigration separation techniques have become powerful techniques, which can provide highly efficient separations and large peak capacities. Today the availability of sophisticated automated equipment for CE makes the technique suitable to be implement in routine laboratories for separation of a variety of molecules including metal ions, organic and inorganic ions, drugs, peptides, proteins and DNA fragments, even whole bacterial cells and viruses. All these parameters predict CE as one of the method of choice also for determination of enzyme activities.
ER  -

VYTISKOVÁ, Soňa a Zdeněk GLATZ. CAPILLARY ELECTROPHORESIS AS A TOOL FOR THE STUDY OF ENZYMES. In \textit{Proceeding of 15th Internationa Conference ''Chromatographic Methods and Human Health''}. Bratislava: Slovenská chemická společnost, 2003, s.~30-30. ISSN~1335-5236.

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