Separation of Polycyclic Aromatic Nitrogen Heterocycles on
Different Octadecylsilica Stationary Phases

D 2003

Separation of Polycyclic Aromatic Nitrogen Heterocycles on Different Octadecylsilica Stationary Phases

KOČÍ, Kamila a Zdeněk ŠIMEK

Základní údaje

Originální název

Separation of Polycyclic Aromatic Nitrogen Heterocycles on Different Octadecylsilica Stationary Phases

Název česky

Separace polycyklyckých aromatických uhlovodíků na různých reversních fázích

Autoři

KOČÍ, Kamila (203 Česká republika) a Zdeněk ŠIMEK (203 Česká republika, garant)

Vydání

Siófok, Hungary, 5th Balaton Symphosyum on High Performance Separation Methods, od s. 53-53, 1 s. 2003

Nakladatel

Research Institute for Medical Plants

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Maďarsko

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Polycyclic Aromatic Nitrogen Heterocycles; Octadecylsilica Stationary Phases

Štítky

Octadecylsilica Stationary Phases, Polycyclic Aromatic Nitrogen Heterocycles

Změněno: 31. 1. 2006 16:18, doc. RNDr. Zdeněk Šimek, CSc.

Anotace

V originále

his study deals with the separation of basic polycyclic aromatic nitrogen heterocycles (PANH) by reversed-phase high-performance liquid chromatography (RP-HPLC) on two octadecylsilane stationary phases made by different manufacturers. The aims of our investigation were to evaluate surface properties of the selected sorbents (hydrophobicity and silanol activity) and to compare the retention behavior of tested PANH on these stationary phases. Experiments were carried out with the following members of basic PANH: quinoline and its mono-methyl derivatives, isoquinoline, benzo[h]quinoline, acridine and phenanthridine. Zorbax Eclipse XDB C18 (Agilent Technologies) and Purospher Star RP-18e (Merck) both in 250 x 4,6 mm package with 5 um i.d. were used as octadecylsilane stationary phases. The evaluation procedures as well as the separation experiments studying retention behavior of basic PANH were carried out under isocratic elution conditions. Evaluation of both reversed-phase sorbents was performed via empirical method based on chromatographic data of the test compounds chosen according to the hydrophobicity and silanol activity of tested sorbents surfaces. During separation experiments several different elution conditions were applied on each column. Especially buffer concentration its pH and ionic strength in conjunction with pKa values of individual heterocycles were taken into consideration.

Návaznosti

GA525/03/0367, projekt VaV

Název: Ekotoxikologie persistentních organických polutantů životního prostředí

Investor: Grantová agentura ČR, Ekotoxikologie persistentních organických polutantů životního prostředí

Citovat

KOČÍ, Kamila a Zdeněk ŠIMEK. Separation of Polycyclic Aromatic Nitrogen Heterocycles on Different Octadecylsilica Stationary Phases. In 5th Balaton Symphosyum on High Performance Separation Methods. Siófok, Hungary: Research Institute for Medical Plants, 2003, s. 53-53.

@inproceedings{491693,
   author = {Kočí, Kamila and Šimek, Zdeněk},
   address = {Siófok, Hungary},
   booktitle = {5th Balaton Symphosyum on High Performance Separation Methods},
   keywords = {Polycyclic Aromatic Nitrogen Heterocycles; Octadecylsilica Stationary Phases},
   language = {eng},
   location = {Siófok, Hungary},
   pages = {53-53},
   publisher = {Research Institute for Medical Plants},
   title = {Separation of Polycyclic Aromatic Nitrogen Heterocycles on Different Octadecylsilica Stationary Phases},
   year = {2003}
}

TY  - JOUR
ID  - 491693
AU  - Kočí, Kamila - Šimek, Zdeněk
PY  - 2003
TI  - Separation of Polycyclic Aromatic Nitrogen Heterocycles on Different Octadecylsilica Stationary Phases
PB  - Research Institute for Medical Plants
CY  - Siófok, Hungary
KW  - Polycyclic Aromatic Nitrogen Heterocycles
KW  - Octadecylsilica Stationary Phases
N2  - his study deals with the separation of basic polycyclic aromatic nitrogen heterocycles (PANH) by reversed-phase high-performance liquid chromatography (RP-HPLC) on two octadecylsilane stationary phases made by different manufacturers. The aims of our investigation were to evaluate surface properties of the selected sorbents (hydrophobicity and silanol activity) and to compare the retention behavior of tested PANH on these stationary phases. Experiments were carried out with the following members of basic PANH: quinoline and its mono-methyl derivatives, isoquinoline, benzo[h]quinoline, acridine and phenanthridine. Zorbax Eclipse XDB C18 (Agilent Technologies) and Purospher Star RP-18e (Merck) both in 250 x 4,6 mm package with 5 um i.d. were used as octadecylsilane stationary phases. The evaluation procedures as well as the separation experiments studying retention behavior of basic PANH were carried out under isocratic elution conditions. Evaluation of both reversed-phase sorbents was performed via empirical method based on chromatographic data of the test compounds chosen according to the hydrophobicity and silanol activity of tested sorbents surfaces. During separation experiments several different elution conditions were applied on each column. Especially buffer concentration its pH and ionic strength in conjunction with pKa values of individual heterocycles were taken into consideration.
ER  -

KOČÍ, Kamila a Zdeněk ŠIMEK. Separation of Polycyclic Aromatic Nitrogen Heterocycles on Different Octadecylsilica Stationary Phases. In \textit{5th Balaton Symphosyum on High Performance Separation Methods}. Siófok, Hungary: Research Institute for Medical Plants, 2003, s.~53-53.

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