Identification of European diplozoids (Diplozoidae, Monogenea)
using the RFLP pattern of second internal transcribed spacer
(ITS2)

J 2004

Identification of European diplozoids (Diplozoidae, Monogenea) using the RFLP pattern of second internal transcribed spacer (ITS2)

MATĚJUSOVÁ, Iveta; Božena KOUBKOVÁ a Carey O. CUNNINGHAM

Základní údaje

Originální název

Identification of European diplozoids (Diplozoidae, Monogenea) using the RFLP pattern of second internal transcribed spacer (ITS2)

Název česky

Druhova identifikace evropskych diplozoonu (Diplozoidae, Monogenea) s pouzitim RFLP ITS2 rDNA

Autoři

MATĚJUSOVÁ, Iveta; Božena KOUBKOVÁ a Carey O. CUNNINGHAM

Vydání

Journal of Parasitology, 2004, 0022-3395

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.439

Kód RIV

RIV/00216224:14310/04:00009908

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

diplozoids; ITS2; genetic markers; maximum likelihood

Štítky

diplozoids, genetic markers, ITS2, maximum likelihood

Změněno: 30. 5. 2005 14:11, Mgr. Iveta Matějusová, Ph.D.

Anotace

ORIG CZ

V originále

The second internal transcribed spacer (ITS2) of the ribosomal RNA genes of Diplozoon paradoxum and Paradiplozoon nagibinae were amplified and sequenced. The PCR product of D. paradoxum was bigger (840bp) than that of P. nagibinae (820bp). There was no intraspecific variability recorded in sequences from either species. Sequence comparisons and ITS2 RFLP pattern of eight European diplozoid species aimed to resolve their identification and amends the previous studies. Restriction fragment length polymorphism (RFLP) was used to distinguish both species from each other and from P. bliccae, P. homoion, P. megan, P. pavlovskii, P. sapae and Eudiplozoon nipponicum, using restriction enzymes AluI, HaeIII, HinfI, RsaI and SphI. The criteria for morphological identification of eight European diplozoids are also included with the main morphological characters of clamps, trapeze spur and anterior joining sclerites of eight diplozoid species being illustrated. Combination of the shape and comparison of length of the trapeze spur and anterior joining sclerites could lead to accurate identification of diplozoid species.

Česky

ITS2 rDNA byla sekvenovana pro druhy Diplozoon paradoxum a Paradiplozoon nagibinae. PCR produkt D. paradoxum byl vetsi (840bp) nez pro P. nagibinae (820bp). Nebyla nalezena vnitrodruhova variabilita v sekvenci obou druhu. Porovnani sekvenci a vysledku RFLP ITS prokazalo existenci osmi druhu evropskych diplozoonu.

Návaznosti

MSM 143100010, záměr

Název: Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy

Citovat

MATĚJUSOVÁ, Iveta; Božena KOUBKOVÁ a Carey O. CUNNINGHAM. Identification of European diplozoids (Diplozoidae, Monogenea) using the RFLP pattern of second internal transcribed spacer (ITS2). Journal of Parasitology. 2004, roč. 90, č. 4, s. 817-822. ISSN 0022-3395.

@article{492322,
   author = {Matějusová, Iveta and Koubková, Božena and Cunningham, Carey O.},
   article_number = {4},
   keywords = {diplozoids; ITS2; genetic markers; maximum likelihood},
   language = {eng},
   issn = {0022-3395},
   journal = {Journal of Parasitology},
   title = {Identification of European diplozoids (Diplozoidae, Monogenea) using the RFLP pattern of second internal transcribed spacer (ITS2)},
   volume = {90},
   year = {2004}
}

TY  - JOUR
ID  - 492322
AU  - Matějusová, Iveta - Koubková, Božena - Cunningham, Carey O.
PY  - 2004
TI  - Identification of European diplozoids (Diplozoidae, Monogenea) using the RFLP pattern of second internal transcribed spacer (ITS2)
JF  - Journal of Parasitology
VL  - 90
IS  - 4
SP  - 817-822
EP  - 817-822
SN  - 00223395
KW  - diplozoids
KW  - ITS2
KW  - genetic markers
KW  - maximum likelihood
N2  - The second internal transcribed spacer (ITS2) of the ribosomal RNA genes of Diplozoon paradoxum and Paradiplozoon nagibinae were amplified and sequenced. The PCR product of D. paradoxum was bigger (840bp) than that of P. nagibinae (820bp). There was no intraspecific variability recorded in sequences from either species. Sequence comparisons and ITS2 RFLP pattern of eight European diplozoid species aimed to resolve their identification and amends the previous studies. Restriction fragment length polymorphism (RFLP) was used to distinguish both species from each other and from P. bliccae, P. homoion, P. megan, P. pavlovskii, P. sapae and Eudiplozoon nipponicum, using restriction enzymes AluI, HaeIII, HinfI, RsaI and SphI. The criteria for morphological identification of eight European diplozoids are also included with the main morphological characters of clamps, trapeze spur and anterior joining sclerites of eight diplozoid species being illustrated. Combination of the shape and comparison of length of the trapeze spur and anterior joining sclerites could lead to accurate identification of diplozoid species.
ER  -

MATĚJUSOVÁ, Iveta; Božena KOUBKOVÁ a Carey O. CUNNINGHAM. Identification of European diplozoids (Diplozoidae, Monogenea) using the RFLP pattern of second internal transcribed spacer (ITS2). \textit{Journal of Parasitology}. 2004, roč.~90, č.~4, s.~817-822. ISSN~0022-3395.

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