2003
Tumor suppressive effects of the p53 protein in v-myb-transformed monoblasts BM2
NAVRÁTILOVÁ, Jarmila, Bořivoj VOJTĚŠEK a Jan ŠMARDAZákladní údaje
Originální název
Tumor suppressive effects of the p53 protein in v-myb-transformed monoblasts BM2
Název anglicky
Tumor suppressive effects of the p53 protein in v-myb-transformed monoblasts BM2
Autoři
NAVRÁTILOVÁ, Jarmila (203 Česká republika), Bořivoj VOJTĚŠEK (203 Česká republika) a Jan ŠMARDA (203 Česká republika, garant)
Vydání
Vyškov, Memory in Living Systems, s. 65-65, 2003
Nakladatel
Czechoslovak Biological Society
Další údaje
Jazyk
čeština
Typ výsledku
Stať ve sborníku
Obor
Genetika a molekulární biologie
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/03:00009568
Organizační jednotka
Přírodovědecká fakulta
ISBN
80-210-3264-2
Klíčová slova anglicky
p53; Myb; proliferation
Štítky
Změněno: 22. 4. 2004 17:04, prof. RNDr. Jan Šmarda, CSc.
V originále
The v-myb oncogene of AMV causes rapid fatal monoblastic leukemia in chickens. In order to explore the ways to suppress transforming capabilities of the v-Myb we ectopically expressed p53 cDNA in AMV v-myb-transformed chicken monoblasts BM2. p53 can induce expression of multiple genes involved in control of cellular proliferation and apoptosis. Usually, the intracellular concentration of p53 protein is maintained at a very low level. However, DNA damage and other stress stimuli stabilize p53 increasing its cellular concentration. We transfected BM2 cells with human p53 cDNA under control inducible promoter and purified clones of stable transfectants. Stability of human p53 protein in this chicken cells was up-regulated by treatment with roscovitine and adriamycine. p53 did not arrest BM2 cell cycle but it increased apoptosis. Interestingly, induction of apoptosis occurred in p53-expressing BM2 cells in the absence of activation of bax, mdm2 and p21WAF/Cip1.
Anglicky
The v-myb oncogene of AMV causes rapid fatal monoblastic leukemia in chickens. In order to explore the ways to suppress transforming capabilities of the v-Myb we ectopically expressed p53 cDNA in AMV v-myb-transformed chicken monoblasts BM2. p53 can induce expression of multiple genes involved in control of cellular proliferation and apoptosis. Usually, the intracellular concentration of p53 protein is maintained at a very low level. However, DNA damage and other stress stimuli stabilize p53 increasing its cellular concentration. We transfected BM2 cells with human p53 cDNA under control inducible promoter and purified clones of stable transfectants. Stability of human p53 protein in this chicken cells was up-regulated by treatment with roscovitine and adriamycine. p53 did not arrest BM2 cell cycle but it increased apoptosis. Interestingly, induction of apoptosis occurred in p53-expressing BM2 cells in the absence of activation of bax, mdm2 and p21WAF/Cip1.
Návaznosti
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