J 2004

Chemiluminescence of lucigenin is dependent on experimental conditions

HYRŠL, Pavel; Antonín LOJEK; Milan ČÍŽ a Lukáš KUBALA

Základní údaje

Originální název

Chemiluminescence of lucigenin is dependent on experimental conditions

Název anglicky

Chemiluminescence of lucigenin is dependent on experimental conditions

Autoři

Vydání

Luminescence, 2004, 1522-7235

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.297

Označené pro přenos do RIV

Ne

Organizační jednotka

Přírodovědecká fakulta

UT WoS

Klíčová slova anglicky

luminescence; lucigenin

Štítky

Změněno: 28. 4. 2011 15:51, doc. RNDr. Pavel Hyršl, Ph.D.

Anotace

V originále

The aim of the study was to test the effect of experimental conditions such as light radiation and temperature on chemiluminescence (CL) of 10-2-10-5 mol/L lucigenin dissolved in various types of solvents. Irradiation by UV light (280, 297, 313 or 400 nm) induced a significant increase in CL of lucigenin dissolved in borate buffer. This effect was the most obvious for 10-2-10-3 mol/L lucigenin. All wavelengths used had a similar effect. UV irradiation did not induce changes in the CL activity of lucigenin dissolved in dH2O or in dimethyl sulphoxide (DMSO). Different results for various solvents were not dependent on pH. The CL activity of 10-2 mol/L and 10-3 mol/L lucigenin dissolved in borate buffer increased depending on the solution temperature (25C, 30C, 37C or 40C) already at the beginning of the analysis, with a further increase during 16 h incubation period. It can be summarized that temperatures higher than 25C and intensive light irradiation are among those factors which significantly affect the result of analysis when lucigenin is used as a luminophor. Copyright Š 2004 John Wiley & Sons, Ltd.