J 2004

Silkworm (Bombyx mori) hemocytes do not produce reactive oxygen metabolites as a part of defence mechanisms

HYRŠL, Pavel; Milan ČÍŽ; Lukáš KUBALA a Antonín LOJEK

Základní údaje

Originální název

Silkworm (Bombyx mori) hemocytes do not produce reactive oxygen metabolites as a part of defence mechanisms

Název anglicky

Silkworm (Bombyx mori) hemocytes do not produce reactive oxygen metabolites as a part of defence mechanisms

Autoři

Vydání

Folia Microbiologica, 2004

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ne

Organizační jednotka

Přírodovědecká fakulta

UT WoS

Klíčová slova anglicky

Bombyx mori; hemocytes; ROM

Štítky

Změněno: 28. 4. 2011 15:51, doc. RNDr. Pavel Hyršl, Ph.D.

Anotace

V originále

The aim of this study was to investigate whether hemocytes of Bombyx mori (Lepidoptera) larvae can produce reactive oxygen species (ROS) as a tool for the oxidative killing of invading pathogens. The production of ROS measured as a luminol- and lucigenin-enhanced chemiluminescence of unstimulated or stimulated (zymosan particles, phorbol myristate acetate, calcium ionophore, rice starch or Xenorhabdus nematophila) hemolymph was not detectable. To eliminate the influence of the antioxidative potential of hemolymph plasma (extremely high when measured by the chemiluminescence method), isolated hemocytes were also tested. However, spontaneous as well as the activated generation of ROS by isolated hemocytes remained at the background level. No ROS production by isolated hemocytes was observed through spectrophotometric (NBT test, cytochrome c assay) and fluorometric (using dihydrorhodamine and hydroethidine probes) analyses. To conclude, none of the several experimental approaches used in this study indicated the production of ROS by hemocytes of Bombyx mori larvae as a part of their immune response.