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@inproceedings{555436,
author = {Hradilová, Jana and Brzobohatý, Břetislav},
address = {Brno},
booktitle = {VIII.Pracovní setkání biochemik a molekulárních biolog},
edition = {1.},
keywords = {ALTERNATIVE SPLICING; PHOSPHOTRANSFER PROTEIN; ARABIDOPSIS THALIANA},
language = {cze},
location = {Brno},
isbn = {80-210-3321-5},
pages = {25-26},
publisher = {Masarykova univerzita v Brn},
title = {TISSUE SPECIFIC ALTERNATIVE SPLICING IN THE GENE FOR AHP5, A HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN FROM ARABIDOPSIS THALIANA},
year = {2004}
}
TY - JOUR
ID - 555436
AU - Hradilová, Jana - Brzobohatý, Břetislav
PY - 2004
TI - TISSUE SPECIFIC ALTERNATIVE SPLICING IN THE GENE FOR AHP5, A HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN FROM ARABIDOPSIS THALIANA
PB - Masarykova univerzita v Brn
CY - Brno
SN - 8021033215
KW - ALTERNATIVE SPLICING
KW - PHOSPHOTRANSFER PROTEIN
KW - ARABIDOPSIS THALIANA
N2 - A multistep two-component system is involved in signal transduction in Arabidopsis and consists of a sensory histidine kinase, a phosphotransfer factor and a response regulator. Factors with histidine-containing phosphotransfer domains (AHPs) transfer the phosphoryl group from membrane localised receptor (histidine kinases) to effectors (response regulators) localised in the nucleus. Five AHPs have been identified in Arabidopsis. AHPs are small proteins (14,5 18 kDa), with a predicted acidic isoeletric point (5.53.9) and a typical phosphotransfer domain, including the conserved sequence XHQXKGSSXS. AHP5 comprises six exons and five introns. RNA was prepared using Trizol reagent from different plant tissues: leaves, roots, shoot, flowers, siliques and seedlings. Using RT-PCR we detected two products (AHP5s and AHP5l). Following sequencing, the second intron was found intact at position 211 bp in the longer product (AHP5l). Real time RT-PCR showed that the ratio between spliced and nonspliced products is in tested tissues significant different. Other AHPs (AHP1, AHP2, AHP3 and AHP4) were established to be normally spliced. AHP5l comprises two open reading frames, and in the longer of them the phosphotransfer domain is preserved intact as the original reading frame is not altered. This protein has a predicted molecular mass of 11.7kDa and the putative isoeletric point is 7.72.
ER -
HRADILOVÁ, Jana a Břetislav BRZOBOHATÝ. TISSUE SPECIFIC ALTERNATIVE SPLICING IN THE GENE FOR AHP5, A HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN FROM ARABIDOPSIS THALIANA. In \textit{VIII.Pracovní setkání biochemik a molekulárních biolog}. 1. vyd. Brno: Masarykova univerzita v Brn, 2004, s.~25-26. ISBN~80-210-3321-5.
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