Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry

PREISLER, Jan, Patrik VRÁBEL, Jaromíra NOVÁKOVÁ, Michal SPĚŠNÝ, Zbyněk ZDRÁHAL, Hana KONEČNÁ, Pavel KRÁSENSKÝ, Luděk EYER, Jiří DOŠKAŘ, Roman PANTŮČEK, František FORET a Karel KLEPÁRNÍK. Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry. In Final Program and Book of Abstracts of 17th International Symposium on Microscale Separations and Capillary Electrophoresis. 2004.

Základní údaje

• Originální název: Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry
• Název česky: Kapilární elektroforéza proteinů bakteriofága 812 kombinovaná s MALDI-hmotnostní spektrometrií
• Autoři: PREISLER, Jan (203 Česká republika, garant, domácí), Patrik VRÁBEL (203 Česká republika, domácí), Jaromíra NOVÁKOVÁ (203 Česká republika, domácí), Michal SPĚŠNÝ (203 Česká republika, domácí), Zbyněk ZDRÁHAL (203 Česká republika, domácí), Hana KONEČNÁ (203 Česká republika, domácí), Pavel KRÁSENSKÝ (203 Česká republika, domácí), Luděk EYER (203 Česká republika, domácí), Jiří DOŠKAŘ (203 Česká republika), Roman PANTŮČEK (203 Česká republika, domácí), František FORET (203 Česká republika) a Karel KLEPÁRNÍK (203 Česká republika).
• Vydání: Final Program and Book of Abstracts of 17th International Symposium on Microscale Separations and Capillary Electrophoresis, 2004.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: 10406 Analytical chemistry
• Stát vydavatele: Rakousko
• Utajení: není předmětem státního či obchodního tajemství
• WWW: URL
• Kód RIV: RIV/00216224:14310/04:00011440
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova anglicky: Capillary Electrophoresis; Proteins; Bacteriophage
• Štítky: Bacteriophage, Capillary electrophoresis, proteins
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification.

Anotace česky

The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification. Anotace anglicky: The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification.

Návaznosti

• GA203/03/0515, projekt VaV: Název: Integrovaná analýza genomu a proteomu terapeuticky významných bakteriofágů kombinací elektroforézy a hmotnostní spektrometrie

Investor: Grantová agentura ČR, Integrovaná analýza genomu a proteomu terapeuticky významných bakteriofágů kombinací elektroforézy a hmotnostní spektrometrie