Development of the piezoelectric biosensor for acetochlor
detection

J 2003

Development of the piezoelectric biosensor for acetochlor detection

LEBEDEV, Michail, Sergei EREMIN a Petr SKLÁDAL

Základní údaje

Originální název

Development of the piezoelectric biosensor for acetochlor detection

Autoři

LEBEDEV, Michail (643 Rusko), Sergei EREMIN (643 Rusko) a Petr SKLÁDAL (203 Česká republika, garant)

Vydání

Analytical Letters, New York, Marcel Dekker, 2003, 0003-2719

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Rusko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.929

Kód RIV

RIV/00216224:14310/03:00009629

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000185235800008

Klíčová slova anglicky

Piezoelectric sensor; Immunosensor; Acetochlor

Štítky

Acetochlor, immunosensor, Piezoelectric sensor

Změněno: 31. 5. 2004 17:06, prof. RNDr. Petr Skládal, CSc.

Anotace

V originále

The piezoelectric immunosensor for the determination of acetochlor was developed. The surface of gold electrodes of piezoelectric quartz crystals was modified by self-assembled thiolayers using either 4-aminothiophenol or dithiobis(succinimidyl propionate). In the next step, the modified surface was used for the coupling of acetochlor-protein conjugates. Acetochlor was conjugated to ovalbumin using either thiopropionic acid or acetylthiosuccinimidyl anhydride (AMSA). The acetochlor-modified crystals were used for characterization of the anti-acetochlor polyclonal antibody (Ab). The kinetic rate and equilibrium constants were compared for both types of immobilization. For acetochlor immobilized through AMSA, the dissociation rate constant was 20-times lower. The possibility of using this system for the competitive determination of free acetochlor in water was further studied. The detection limit (10% decrease of relative binding of the antibody) was 0.20 mg/l). The piezoelectric crystals were used repeatedly, 100 mM formic acid served for regeneration of the sensing surface. The total time for one measurement was about 30 min including 15 min pre-incubation of antibody with sample, 10 min binding reaction and 4 min regeneration.

Návaznosti

OC 518.30, projekt VaV

Název: Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů

Citovat

LEBEDEV, Michail, Sergei EREMIN a Petr SKLÁDAL. Development of the piezoelectric biosensor for acetochlor detection. Analytical Letters. New York: Marcel Dekker, 2003, roč. 36, č. 10, s. 2443-2457. ISSN 0003-2719.

@article{555470,
   author = {Lebedev, Michail and Eremin, Sergei and Skládal, Petr},
   article_location = {New York},
   article_number = {10},
   keywords = {Piezoelectric sensor; Immunosensor; Acetochlor},
   language = {eng},
   issn = {0003-2719},
   journal = {Analytical Letters},
   title = {Development of the piezoelectric biosensor for acetochlor detection},
   volume = {36},
   year = {2003}
}

TY  - JOUR
ID  - 555470
AU  - Lebedev, Michail - Eremin, Sergei - Skládal, Petr
PY  - 2003
TI  - Development of the piezoelectric biosensor for acetochlor detection
JF  - Analytical Letters
VL  - 36
IS  - 10
SP  - 2443-2457
EP  - 2443-2457
PB  - Marcel Dekker
SN  - 00032719
KW  - Piezoelectric sensor
KW  - Immunosensor
KW  - Acetochlor
N2  - The piezoelectric immunosensor for the determination of acetochlor was developed. The surface of gold electrodes of piezoelectric quartz crystals was modified by self-assembled thiolayers using either 4-aminothiophenol or dithiobis(succinimidyl propionate). In the next step, the modified surface was used for the coupling of acetochlor-protein conjugates. Acetochlor was conjugated to ovalbumin using either thiopropionic acid or acetylthiosuccinimidyl anhydride (AMSA). The acetochlor-modified crystals were used for characterization of the anti-acetochlor polyclonal antibody (Ab). The kinetic rate and equilibrium constants were compared for both types of immobilization. For acetochlor immobilized through AMSA, the dissociation rate constant was 20-times lower. The possibility of using this system for the competitive determination of free acetochlor in water was further studied. The detection limit (10% decrease of relative binding of the antibody) was 0.20 mg/l). The piezoelectric crystals were used repeatedly, 100 mM formic acid served for regeneration of the sensing surface. The total time for one measurement was about 30 min including 15 min pre-incubation of antibody with sample, 10 min binding reaction and 4 min regeneration.
ER  -

LEBEDEV, Michail, Sergei EREMIN a Petr SKLÁDAL. Development of the piezoelectric biosensor for acetochlor detection. \textit{Analytical Letters}. New York: Marcel Dekker, 2003, roč.~36, č.~10, s.~2443-2457. ISSN~0003-2719.

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