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@proceedings{555527,
author = {David, Marek and Gabriel, Miroslav and Kopecká, Marie},
booktitle = {30nd Annual Conference on Yeasts},
keywords = {lipophilic yeast; Malassezia pachydermatis; transmission electron microscopy; ultrastructure},
language = {eng},
title = {The study of potentially pathogenic lipophilic yeast Malassezia pachydermatis},
year = {2002}
}
TY - CONF
ID - 555527
AU - David, Marek - Gabriel, Miroslav - Kopecká, Marie
PY - 2002
TI - The study of potentially pathogenic lipophilic yeast Malassezia pachydermatis
KW - lipophilic yeast
KW - Malassezia pachydermatis
KW - transmission electron microscopy
KW - ultrastructure
N2 - The potential human pathogen M. pachydermatis was studied to identify cytoskeleton as potential therapeutic target for antifungal agents. Light and transmission electron microscopy (TEM) confirmed the small cells (2,0-3,0 x 4,0-5,0 mm) and few intracellular structures. TEM confirmed the presence of thick multilayered cell wall that appeared to be a barrier for fluorescent antibodies. The higher concentration of detergent and lytical wall enzymes, the prolonged time of their and fluorescent antibodies applications seem necessary prerequisites to detect cytoskeleton. Preliminary results showed poor cytoskeletal structures. This report confirmed multilayered cell wall, the deep and helicoidal invaginations of plasma membrane and few intracellular structures. Cytoskeleton will be further studied by fluorescence and immunofluorescence and by TEM. The research was supported by grant No. 310/00/0391 of the Grant Agency of Czech Republic.
ER -
DAVID, Marek, Miroslav GABRIEL a Marie KOPECKÁ. The study of potentially pathogenic lipophilic yeast Malassezia pachydermatis. In \textit{30nd Annual Conference on Yeasts}. 2002.
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