Occurrence of Borrelia afzelii and Borrelia garinii in Ixodes
ricinus Ticks from Southern Moravia, Czech Republic

J 2004

Occurrence of Borrelia afzelii and Borrelia garinii in Ixodes ricinus Ticks from Southern Moravia, Czech Republic

JANOUŠKOVCOVÁ, Eva; Alena ŽÁKOVSKÁ; Jiří HALOUZKA a Miloš DENDIS

Základní údaje

Originální název

Occurrence of Borrelia afzelii and Borrelia garinii in Ixodes ricinus Ticks from Southern Moravia, Czech Republic

Název česky

Přítomnost of Borrelia afzelii a Borrelia garinii v Ixodes ricinus na jižní Moravě, Czech Republic

Autoři

JANOUŠKOVCOVÁ, Eva; Alena ŽÁKOVSKÁ; Jiří HALOUZKA a Miloš DENDIS

Vydání

Vector-Borne and Zoonotic Diseases, New York, USA, Mary Ann Liebert, Inc. 2004, 1530-3667

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/04:00025485

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000220590400005

Klíčová slova anglicky

Ixodes ricinus Borrelia afzelii Borrelia garinii Prevalence Identification PCR-RFLP SDS-PAGE

Příznaky

Recenzováno

Změněno: 23. 6. 2009 13:53, doc. RNDr. Alena Žákovská, Ph.D.

Anotace

ORIG CZ

V originále

During the years 1996 - 2000, a total of 2398 Ixodes ricinus ticks were collected in three areas of southern Moravia and eastern Bohemia, Czech Republic, and examined by dark-field microscopy for the presence of spirochetes. The prevalence of B. burgdorferi s. l. in Ixodes ricinus ticks varied and depended upon the year. In 1996 the prevalence 6.8 % was observed, during 1997 and 1998 it increased to 8.4 % and 12.3 %, respectively. The lowest prevalence was observed in 1999 (3.6 %) and in 2000 it increased to 4.0 %. The mean rate of infection was 6.5 % and the proportions of infected ticks were 12.2 % in 263 male ticks, 8.3 % in 289 female ticks, 6.0 % in 1621 nymphs and 1.3 % in 225 larvae. From the total of 156 highly infected ticks (>100 spirochetes per sample) transferred into BSK-H medium for isolation attempts, thirteen isolates were obtained. PCR-RFLP and electrophoresis (SDS-PAGE) were used for the identification and characterization of Borrelia strains. Ten tick isolates were identified as Borrelia afzelii and the other three isolates were found to be Borrelia garinii. The results indicate the epidemiological importance of B. afzelii and B. garinii in central Europe and emphasize the role of I. ricinus in the ecology of B. burgdorferi and in the epidemiology and epizootiology of Lyme disease.

Česky

Přítomnost of Borrelia afzelii a Borrelia garinii v Ixodes ricinus na jižní Moravě, Czech Republic, PCR metoda

Návaznosti

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

MSM 143100010, záměr

Název: Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy

Citovat

JANOUŠKOVCOVÁ, Eva; Alena ŽÁKOVSKÁ; Jiří HALOUZKA a Miloš DENDIS. Occurrence of Borrelia afzelii and Borrelia garinii in Ixodes ricinus Ticks from Southern Moravia, Czech Republic. Vector-Borne and Zoonotic Diseases. New York, USA: Mary Ann Liebert, Inc., 2004, roč. 4, č. 1, s. 43-52. ISSN 1530-3667.

@article{555612,
   author = {Janouškovcová, Eva and Žákovská, Alena and Halouzka, Jiří and Dendis, Miloš},
   article_location = {New York, USA},
   article_number = {1},
   keywords = {Ixodes ricinus Borrelia afzelii Borrelia garinii Prevalence Identification PCR-RFLP SDS-PAGE},
   language = {eng},
   issn = {1530-3667},
   journal = {Vector-Borne and Zoonotic Diseases},
   title = {Occurrence of Borrelia afzelii and Borrelia garinii in Ixodes ricinus Ticks from Southern Moravia, Czech Republic},
   volume = {4},
   year = {2004}
}

TY  - JOUR
ID  - 555612
AU  - Janouškovcová, Eva - Žákovská, Alena - Halouzka, Jiří - Dendis, Miloš
PY  - 2004
TI  - Occurrence of Borrelia afzelii and Borrelia garinii in Ixodes ricinus Ticks from Southern Moravia, Czech Republic
JF  - Vector-Borne and Zoonotic Diseases
VL  - 4
IS  - 1
SP  - 43-52
EP  - 43-52
PB  - Mary Ann Liebert, Inc.
SN  - 15303667
KW  - Ixodes ricinus Borrelia afzelii Borrelia garinii Prevalence Identification PCR-RFLP SDS-PAGE
N2  - During the years 1996 - 2000, a total of 2398 Ixodes ricinus ticks were collected in three areas of southern Moravia and eastern Bohemia, Czech Republic, and examined by dark-field microscopy for the presence of spirochetes. The prevalence of B. burgdorferi s. l. in Ixodes ricinus ticks varied and depended upon the year. In 1996 the prevalence 6.8 % was observed, during 1997 and 1998 it increased to 8.4 % and 12.3 %, respectively. The lowest prevalence was observed in 1999 (3.6 %) and in 2000 it increased to 4.0 %. The mean rate of infection was 6.5 % and the proportions of infected ticks were 12.2 % in 263 male ticks, 8.3 % in 289 female ticks, 6.0 % in 1621 nymphs and 1.3 % in 225 larvae. From the total of 156 highly infected ticks (>100 spirochetes per sample) transferred into BSK-H medium for isolation attempts, thirteen isolates were obtained. PCR-RFLP and electrophoresis (SDS-PAGE) were used for the identification and characterization of Borrelia strains. Ten tick isolates were identified as Borrelia afzelii and the other three isolates were found to be Borrelia garinii. The results indicate the epidemiological importance of B. afzelii and B. garinii in central Europe and emphasize the role of I. ricinus in the ecology of B. burgdorferi and in the epidemiology and epizootiology of Lyme disease.
ER  -

JANOUŠKOVCOVÁ, Eva; Alena ŽÁKOVSKÁ; Jiří HALOUZKA a Miloš DENDIS. Occurrence of Borrelia afzelii and Borrelia garinii in Ixodes ricinus Ticks from Southern Moravia, Czech Republic. \textit{Vector-Borne and Zoonotic Diseases}. New York, USA: Mary Ann Liebert, Inc., 2004, roč.~4, č.~1, s.~43-52. ISSN~1530-3667.

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