Sorting inhibitors (Sortins): Chemical compounds to study
vacuolar sorting in Arabidopsis

J 2004

Sorting inhibitors (Sortins): Chemical compounds to study vacuolar sorting in Arabidopsis

ZOUHAR, Jan, Glenn R HICKS a Natasha V RAIKHEL

Základní údaje

Originální název

Sorting inhibitors (Sortins): Chemical compounds to study vacuolar sorting in Arabidopsis

Autoři

ZOUHAR, Jan, Glenn R HICKS a Natasha V RAIKHEL

Vydání

Proceedings of the National Academy of Sciences of the U.S.A. 2004, 0337543100

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000222278600060

Klíčová slova anglicky

chemical genomics; vacuole; yeast; Arabidopsis

Štítky

Arabidopsis, chemical genomics, Vacuole, Yeast

Změněno: 24. 6. 2004 00:38, doc. Mgr. Jan Zouhar, Ph.D.

Anotace

V originále

Chemical genomics is an interdisciplinary approach that unites the power of chemical screens and genomics strategies to dissect biological processes such as endomembrane trafficking. We have taken advantage of the evolutionary conservation between plants and Saccharomyces cerevisiae to identify such chemicals. Using S. cerevisiae, we screened a library of diverse chemical structures for compounds that induce the secretion of carboxypeptidase Y, which is normally targeted to the vacuole. Among 4,800 chemicals screened, 14 compounds, termed sorting inhibitors (Sortins), were identified that stimulated secretion in yeast. In Arabidopsis seedlings, application of Sortin1 and -2 led to reversible defects in vacuole biogenesis and root development. Sortin1 was found to redirect the vacuolar destination of plant carboxypeptidase Y and other proteins in Arabidopsis suspension cells and cause these proteins to be secreted. Sortin1 treatment of whole Arabidopsis seedlings also resulted in carboxypeptidase Y secretion, indicating that the drug has a similar mode of action in cells and intact plants. We have demonstrated that screening of a simple eukaryote, in which vacuolar biogenesis is not essential, can be a powerful tool to find chemicals that interfere with vacuolar delivery of proteins in plants, where vacuole biogenesis is essential. Our studies were done by using a sublethal dose of Sortin1, demonstrating the powerful ability of the chemical to control the induced phenotype in a manner that would be difficult to achieve using conventional genetics.

Citovat

ZOUHAR, Jan, Glenn R HICKS a Natasha V RAIKHEL. Sorting inhibitors (Sortins): Chemical compounds to study vacuolar sorting in Arabidopsis. Proceedings of the National Academy of Sciences of the U.S.A. 2004, roč. 101, č. 25, s. 9497-9501. ISSN 0337543100.

@article{556018,
   author = {Zouhar, Jan and Hicks, Glenn R and Raikhel, Natasha V},
   article_number = {25},
   keywords = {chemical genomics; vacuole; yeast; Arabidopsis},
   language = {eng},
   issn = {0337543100},
   journal = {Proceedings of the National Academy of Sciences of the U.S.A.},
   title = {Sorting inhibitors (Sortins): Chemical compounds to study vacuolar sorting in Arabidopsis},
   url = {http://www.pnas.org/cgi/content/full/101/25/9497},
   volume = {101},
   year = {2004}
}

TY  - JOUR
ID  - 556018
AU  - Zouhar, Jan - Hicks, Glenn R - Raikhel, Natasha V
PY  - 2004
TI  - Sorting inhibitors (Sortins): Chemical compounds to study vacuolar sorting in Arabidopsis
JF  - Proceedings of the National Academy of Sciences of the U.S.A.
VL  - 101
IS  - 25
SP  - 9497-9501
EP  - 9497-9501
SN  - 0337543100
KW  - chemical genomics
KW  - vacuole
KW  - yeast
KW  - Arabidopsis
UR  - http://www.pnas.org/cgi/content/full/101/25/9497
N2  - Chemical genomics is an interdisciplinary approach that unites the power of chemical screens and genomics strategies to dissect biological processes such as endomembrane trafficking. We have taken advantage of the evolutionary conservation between plants and Saccharomyces cerevisiae to identify such chemicals. Using S. cerevisiae, we screened a library of diverse chemical structures for compounds that induce the secretion of carboxypeptidase Y, which is normally targeted to the vacuole. Among 4,800 chemicals screened, 14 compounds, termed sorting inhibitors (Sortins), were identified that stimulated secretion in yeast. In Arabidopsis seedlings, application of Sortin1 and -2 led to reversible defects in vacuole biogenesis and root development. Sortin1 was found to redirect the vacuolar destination of plant carboxypeptidase Y and other proteins in Arabidopsis suspension cells and cause these proteins to be secreted. Sortin1 treatment of whole Arabidopsis seedlings also resulted in carboxypeptidase Y secretion, indicating that the drug has a similar mode of action in cells and intact plants. We have demonstrated that screening of a simple eukaryote, in which vacuolar biogenesis is not essential, can be a powerful tool to find chemicals that interfere with vacuolar delivery of proteins in plants, where vacuole biogenesis is essential. Our studies were done by using a sublethal dose of Sortin1, demonstrating the powerful ability of the chemical to control the induced phenotype in a manner that would be difficult to achieve using conventional genetics.
ER  -

ZOUHAR, Jan, Glenn R HICKS a Natasha V RAIKHEL. Sorting inhibitors (Sortins): Chemical compounds to study vacuolar sorting in Arabidopsis. \textit{Proceedings of the National Academy of Sciences of the U.S.A.}. 2004, roč.~101, č.~25, s.~9497-9501. ISSN~0337543100.

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