| ŠMARDA, Jan, Eva ZAHRADNÍČKOVÁ, Karel SOUČEK a Viktor HORVÁTH. Camptothecin induces autophagy of v-myb-transformed monoblasts. Differentiation. Malden, MA 02148, USA: Blackwell Publishing, 2004, roč. 72, č. 6, s. 279. ISSN 0301-4681. |
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@article{557993,
author = {Šmarda, Jan and Zahradníčková, Eva and Souček, Karel and Horváth, Viktor},
article_location = {Malden, MA 02148, USA},
article_number = {6},
keywords = {Myb; differentiation; programmed cell death; autophagy},
language = {eng},
issn = {0301-4681},
journal = {Differentiation},
title = {Camptothecin induces autophagy of v-myb-transformed monoblasts},
volume = {72},
year = {2004}
}
TY - JOUR
ID - 557993
AU - Šmarda, Jan - Zahradníčková, Eva - Souček, Karel - Horváth, Viktor
PY - 2004
TI - Camptothecin induces autophagy of v-myb-transformed monoblasts
JF - Differentiation
VL - 72
IS - 6
SP - 279
EP - 279
PB - Blackwell Publishing
SN - 03014681
KW - Myb
KW - differentiation
KW - programmed cell death
KW - autophagy
N2 - Programmed cell death (PCD) pathways are frequently damaged during processes of malignant transformation. Abrogation of PCD can significantly affect the response of the cancer cell to a therapy. Therefore, understanding of molecular mechanisms regulating PCD in cancer cells is of potential clinical interest. In this study we analyzed PCD pathways in the cells of two leukemia cell lines: human U937 promonocytes and chicken v-myb-transformed BM2 monoblasts. PCD of these cells was induced using three agents: arsenic trioxide induces PCD by elevation of the intracellular level of hydrogen peroxide, cycloheximide acts as inhibitor of proteosynthesis and camptothecin inhibits activity of DNA topoisomerase I thus causing DNA damage. We found the mechanisms of PCD activated in BM2 and U937 cells by these agents partially different. Under conditions when U937 cells undergo caspase-mediated apoptosis, BM2 cells die by caspase-independent pathway. In addition, DNA damage caused by camptothecin results in a switch from apoptosis to autophagy in BM2 cells. Interestingly, camptothecin fails to induce similar effect in U937 cells. v-myb oncogene of avian myeloblastosis virus codes for regulator of proliferation, differentiation and apoptosis. Our results suggest that v-Myb oncoprotein over-expressed in BM2 but not in U937 cells can be significantly engaged in regulation of programmed cell death pathways in leukemic cells.
ER -
ŠMARDA, Jan, Eva ZAHRADNÍČKOVÁ, Karel SOUČEK a Viktor HORVÁTH. Camptothecin induces autophagy of v-myb-transformed monoblasts. \textit{Differentiation}. Malden, MA 02148, USA: Blackwell Publishing, 2004, roč.~72, č.~6, s.~279. ISSN~0301-4681.
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