Separation of microcystins by capillary electrochromatography
on sol-gel monolithic columns

D 2004

Separation of microcystins by capillary electrochromatography on sol-gel monolithic columns

ZEISBERGEROVÁ, Marta, Markéta ŠRÁMKOVÁ, Zdeněk GLATZ a Vladislav KAHLE

Základní údaje

Originální název

Separation of microcystins by capillary electrochromatography on sol-gel monolithic columns

Název česky

Separace mikrocystinů pomocí CEC v sol-gel monolitických kolonách.

Autoři

ZEISBERGEROVÁ, Marta (203 Česká republika), Markéta ŠRÁMKOVÁ (203 Česká republika), Zdeněk GLATZ (203 Česká republika, garant) a Vladislav KAHLE (203 Česká republika)

Vydání

Řím, Book of abstract 14 th International Symposium on Capillary Electroseparation Techniques ITP 2004, s. 104-104, 2004

Nakladatel

Consiglio Nazionale delle Ricerche

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Itálie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/04:00010321

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

microcystins; capillary electrochromatography

Štítky

Capillary electrochromatography, microcystins

Změněno: 19. 5. 2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.

Anotace

ORIG CZ

V originále

Microcystins belong to a group of monocyclic peptide toxins produced by some cyanobacterial strains. Microcystins are hepatotoxic heptapeptides and have been found to be strong inhibitors of protein phosphatese (type 1 and 2A) and general tumor promoters. They are extremely stable and resistant. These compounds can induce toxocity in seafood or freshwater organisms at very low levels. They are also presented in drinking water reservoirs. Due to the adverse biological and human health effects there is a demand of sensitive method for their detection and determination. In recent years the most employed methods are high performance liquid chromatography (HPLC) and capillary electrophoresis (CE) with UV or mass spectrometry detection. Other possibility is capillary electrochromatography as a modern technique that comprises the main features of CE and HPLC. Thus it seems a promising technique for such application. In this work we concern with electrochromatographic separation of the most abundant cyanobacterial microcystins. The separation of microcystins on C 18 monolithic column with the UV and mass spectrometry detection will be shown.

Česky

Jedná se o separaci mikrocystinů pomocí CEC v sol-gel monolitických kolonách.

Návaznosti

GA203/02/1447, projekt VaV

Název: Kapilární elektrochromatografie v lineárních a planárních mikrostrukturách s využitím polymerních náplní

Investor: Grantová agentura ČR, Kapilární elektrochromatografie v lineárních a planárních mikrostrukturách s využitím polymerních náplní

MSM 143100005, záměr

Název: Strukturně-funkční vztahy biomolekul a jejich role v metabolismu

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Strukturně-funkční vztahy biomolekul a jejich role v metabolismu

Citovat

ZEISBERGEROVÁ, Marta, Markéta ŠRÁMKOVÁ, Zdeněk GLATZ a Vladislav KAHLE. Separation of microcystins by capillary electrochromatography on sol-gel monolithic columns. In Book of abstract 14 th International Symposium on Capillary Electroseparation Techniques ITP 2004. Řím: Consiglio Nazionale delle Ricerche, 2004, s. 104.

@inproceedings{558259,
   author = {Zeisbergerová, Marta and Šrámková, Markéta and Glatz, Zdeněk and Kahle, Vladislav},
   address = {Řím},
   booktitle = {Book of abstract 14 th International Symposium on Capillary Electroseparation Techniques ITP 2004},
   keywords = {microcystins; capillary electrochromatography},
   language = {eng},
   location = {Řím},
   pages = {104-104},
   publisher = {Consiglio Nazionale delle Ricerche},
   title = {Separation of microcystins by capillary electrochromatography on sol-gel monolithic columns},
   year = {2004}
}

TY  - JOUR
ID  - 558259
AU  - Zeisbergerová, Marta - Šrámková, Markéta - Glatz, Zdeněk - Kahle, Vladislav
PY  - 2004
TI  - Separation of microcystins by capillary electrochromatography on sol-gel monolithic columns
PB  - Consiglio Nazionale delle Ricerche
CY  - Řím
KW  - microcystins
KW  - capillary electrochromatography
N2  - Microcystins belong to a group of monocyclic peptide toxins produced by some cyanobacterial strains. Microcystins are hepatotoxic heptapeptides and have been found to be strong inhibitors of protein phosphatese (type 1 and 2A) and general tumor promoters. They are extremely stable and resistant. These compounds can induce toxocity in seafood or freshwater organisms at very low levels. They are also presented in drinking water reservoirs. Due to the adverse biological and human health effects there is a demand of sensitive method for their detection and determination. In recent years the most employed methods are high performance liquid chromatography (HPLC) and capillary electrophoresis (CE) with UV or mass spectrometry detection. Other possibility is capillary electrochromatography as a modern technique that comprises the main features of CE and HPLC. Thus it seems a promising technique for such application. In this work we concern with electrochromatographic separation of the most abundant cyanobacterial microcystins. The separation of microcystins on C 18 monolithic column with the UV and mass spectrometry detection will be shown.
ER  -

ZEISBERGEROVÁ, Marta, Markéta ŠRÁMKOVÁ, Zdeněk GLATZ a Vladislav KAHLE. Separation of microcystins by capillary electrochromatography on sol-gel monolithic columns. In \textit{Book of abstract 14 th International Symposium on Capillary Electroseparation Techniques ITP 2004}. Řím: Consiglio Nazionale delle Ricerche, 2004, s.~104.

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