Effect of endogenous cytokinin levels on the Arabidopsis
thaliana proteome

D 2004

Effect of endogenous cytokinin levels on the Arabidopsis thaliana proteome

VÁŇOVÁ, Pavlína, François CHEVALIER, Gabriela BÖHMOVÁ, Hana KONEČNÁ, Zbyněk ZDRÁHAL et. al.

Basic information

Original name

Effect of endogenous cytokinin levels on the Arabidopsis thaliana proteome

Name in Czech

Vliv hladiny endogenních cytokininů na proteom Arabidopsis thaliana

Authors

VÁŇOVÁ, Pavlína (203 Czech Republic), François CHEVALIER (250 France), Gabriela BÖHMOVÁ (203 Czech Republic), Hana KONEČNÁ (203 Czech Republic), Zbyněk ZDRÁHAL (203 Czech Republic), Michel ROSSIGNOL (250 France) and Břetislav BRZOBOHATÝ (203 Czech Republic, guarantor)

Edition

Olomouc, Chemica 43S Supplement (Collected Reports of the Natural Science Faculty Palacký University Olomouc Czech Republic), p. 24-24, 2004

Publisher

Palacký University

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/04:00025491

Organization unit

Faculty of Science

ISBN

80-244-0882-1

Keywords in English

Arabidopsis; proteomics; cytokinin; 2-D electrophoresis

Abstract

ORIG CZ

V originále

The plant hormone cytokinin plays a crucial role in plant development from germination to senescence. Nevertheless, the exact mechanisms of its influence on these several developmental processes remain unexplained. We report an attempt to reveal more information about the mode of action of cytokinin in the model plant Arabidopsis thaliana. For this purpose a classical proteome-based approach using two-dimensional electrophoretic separation followed by protein digestion and identification by mass spectrometry was established. Total proteins were extracted from whole Arabidopsis plantlets of following genotypes: wild-type ColO, amp1 mutant accumulating six-fold higher level of endogenous cytokinin (1) and pOp-ipt::LhGR transgenic line in which the production of endogenous cytokinin is inducible by dexamethasone (2). All plants were grown for 15 days under long-day conditions (16h light/8h dark) on MS media, in parallel with and without 2.5 mM dexamethasone. Image analysis of two-dimensional gels in triplicates using Progenesis Workstation software and subsequent statistical evaluation revealed both qualitative and quantitative changes in protein expression between plants with elevated cytokinin levels and controls. Out of these differently expressed protein spots so far 21 significant ones were in-gel digested with trypsine and the resulting peptides were subjected to MALDI-TOF MS analysis. Proteins corresponding to the acquired mass spectra were identified in 17 of the analysed spots. Elucidating the relationship of the identified proteins to cytokinin metabolism and action will be a longer-term task. A study of the kinetics of the proteome changes after dexamethasone treatment in pOp-ipt::LhGR plants represents the immediate challenge. This project was supported by the Ministry of Education of the Czech Republic (grant No. MSM 143100008) and by the scholarship IN-CO Czech 2002-2004 within the Leonardo da Vinci programme. References 1. Chaudhury A. M., Letham S., Craig S., and Dennis E.S.: Plant J. 4, 907916 (1993). 2. Moore I., Galweiler L., Grosskopf D., Schell J. and Palme K.: Proc Natl Acad Sci USA 95(1), 376-81 (1998).

In Czech

Rostlinný hormon cytokinin hraje klíčovou úlohu ve vývoji rostlin od klíčení po senescenci, nicméně přesné mechanismy jeho vlivu na tyto vývojové procesy nejsou dosud dostatečně objasněny. Naše studie se pokouší přinést více informací o působení cytokininu u modelové rostliny Arabidopsis thaliana. K tomuto účelu jsme využili klasický proteomický přístup založený na separaci proteinů dvourozměrnou elektroforézou následovanou jejich digescí a identifikací pomocí hmotnostní spektrometrie.

Links

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

VÁŇOVÁ, Pavlína, François CHEVALIER, Gabriela BÖHMOVÁ, Hana KONEČNÁ, Zbyněk ZDRÁHAL, Michel ROSSIGNOL and Břetislav BRZOBOHATÝ. Effect of endogenous cytokinin levels on the Arabidopsis thaliana proteome. In Chemica 43S Supplement (Collected Reports of the Natural Science Faculty Palacký University Olomouc Czech Republic). Olomouc: Palacký University, 2004, p. 24. ISBN 80-244-0882-1.

@inproceedings{558708,
   author = {Váňová, Pavlína and Chevalier, François and Böhmová, Gabriela and Konečná, Hana and Zdráhal, Zbyněk and Rossignol, Michel and Brzobohatý, Břetislav},
   address = {Olomouc},
   booktitle = {Chemica 43S Supplement (Collected Reports of the Natural Science Faculty Palacký University Olomouc Czech Republic)},
   keywords = {Arabidopsis; proteomics; cytokinin; 2-D electrophoresis},
   language = {eng},
   location = {Olomouc},
   isbn = {80-244-0882-1},
   pages = {24-24},
   publisher = {Palacký University},
   title = {Effect of endogenous cytokinin levels on the Arabidopsis thaliana proteome},
   year = {2004}
}

TY  - JOUR
ID  - 558708
AU  - Váňová, Pavlína - Chevalier, François - Böhmová, Gabriela - Konečná, Hana - Zdráhal, Zbyněk - Rossignol, Michel - Brzobohatý, Břetislav
PY  - 2004
TI  - Effect of endogenous cytokinin levels on the Arabidopsis thaliana proteome
PB  - Palacký University
CY  - Olomouc
SN  - 8024408821
KW  - Arabidopsis
KW  - proteomics
KW  - cytokinin
KW  - 2-D electrophoresis
N2  - The plant hormone cytokinin plays a crucial role in plant development from germination to senescence. Nevertheless, the exact mechanisms of its influence on these several developmental processes remain unexplained. We report an attempt to reveal more information about the mode of action of cytokinin in the model plant Arabidopsis thaliana. For this purpose a classical proteome-based approach using two-dimensional electrophoretic separation followed by protein digestion and identification by mass spectrometry was established. Total proteins were extracted from whole Arabidopsis plantlets of following genotypes: wild-type ColO, amp1 mutant accumulating six-fold higher level of endogenous cytokinin (1) and pOp-ipt::LhGR transgenic line in which the production of endogenous cytokinin is inducible by dexamethasone (2). All plants were grown for 15 days under long-day conditions (16h light/8h dark) on MS media, in parallel with and without 2.5 mM dexamethasone. Image analysis of two-dimensional gels in triplicates using Progenesis Workstation software and subsequent statistical evaluation revealed both qualitative and quantitative changes in protein expression between plants with elevated cytokinin levels and controls. Out of these differently expressed protein spots so far 21 significant ones were in-gel digested with trypsine and the resulting peptides were subjected to MALDI-TOF MS analysis. Proteins corresponding to the acquired mass spectra were identified in 17 of the analysed spots. Elucidating the relationship of the identified proteins to cytokinin metabolism and action will be a longer-term task. A study of the kinetics of the proteome changes after dexamethasone treatment in pOp-ipt::LhGR plants represents the immediate challenge. This project was supported by the Ministry of Education of the Czech Republic (grant No. MSM 143100008) and by the scholarship IN-CO Czech 2002-2004 within the Leonardo da Vinci programme. References 1. Chaudhury A. M., Letham S., Craig S., and Dennis E.S.: Plant J. 4, 907916 (1993). 2. Moore I., Galweiler L., Grosskopf D., Schell J. and Palme K.: Proc Natl Acad Sci USA 95(1), 376-81 (1998).
ER  -

VÁŇOVÁ, Pavlína, Fran$\backslash$c cois CHEVALIER, Gabriela BÖHMOVÁ, Hana KONEČNÁ, Zbyněk ZDRÁHAL, Michel ROSSIGNOL and Břetislav BRZOBOHATÝ. Effect of endogenous cytokinin levels on the Arabidopsis thaliana proteome. In \textit{Chemica 43S Supplement (Collected Reports of the Natural Science Faculty Palacký University Olomouc Czech Republic)}. Olomouc: Palacký University, 2004, p.~24. ISBN~80-244-0882-1.

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