Relationship between secondary structure of elicitins,
proteinaceous elicitors of Phytophthora sp. and defense
reaction induced in tobacco cells.

D 2004

Relationship between secondary structure of elicitins, proteinaceous elicitors of Phytophthora sp. and defense reaction induced in tobacco cells.

MIKEŠ, Vladimír, Tomáš KAŠPAROVSKÝ, Jan LOCHMAN, Jiří DAMBORSKY, Michel PONCHET et. al.

Základní údaje

Originální název

Relationship between secondary structure of elicitins, proteinaceous elicitors of Phytophthora sp. and defense reaction induced in tobacco cells.

Název česky

Vztah mezi sekundární strukturou elicitinů, proteinových reaktantů z Phytophthora sp. a obranou reakcí vyvolanou v buňkách tabáku.

Autoři

MIKEŠ, Vladimír (203 Česká republika, garant), Tomáš KAŠPAROVSKÝ (203 Česká republika), Jan LOCHMAN (203 Česká republika), Jiří DAMBORSKY (203 Česká republika), Michel PONCHET (250 Francie) a Jean-Pierre BLEIN (124 Kanada)

Vydání

2004. vyd. Aberdeen, UK, Absract Book of 7th Conference of European Foundation for Plant Pathology 2004, s. 10-10, 2004

Nakladatel

EFPP

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14310/04:00011587

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

cryptogein; plant defense; Phytophthora

Štítky

cryptogein, Phytophthora, plant defense

Změněno: 10. 2. 2005 09:22, prof. RNDr. Vladimír Mikeš, CSc.

Anotace

ORIG CZ

V originále

Elicitins are secreted specifically by the fungi Oomycete genera Pythium and Phytophthora [1, 2]. Biological function of elicitins is currently unknown. The response is induced by the interaction of elicitins with a putative receptor located on the cytoplasmic membrane. The transfer of signal through the receptor triggers phosphorylation-dephosphorylation cascades in the tobacco resulting in alkalization of the extracellular medium, efflux of potassium and chloride ions, influx of calcium, production of the active species from oxygen [3, 4], changes in composition of the cell wall and the induction of acquired systemic resistance. Binding of sterols to the cavity of cryptogein (elicitin from P. cryptogea) seems to be essential for consecutive association of the cryptogein with a receptor and induction of a biological response in a plant [5]. We prepared a series of mutants of cryptogein, one of the most potent elicitors of this group, with altered capacity of binding sterols (L19-R, I63-F, L15-W, L36-F, M35-F, M35-W, M59-W, M59-F). We compared the physicochemical parameters of sterol-cryptogein binding, their ability to induce the synthesis of active oxygen species, ion fluxes, their necrotic activity on tobacco suspension cells and the ability to induce the expression of pathogen related (PR) proteins in plants. The results showed that some of the early events are proportional to the affinity of cryptogein to bind sterols whereas the others (necrotic effect and the induction of PR protein synthesis) seemed to be dependent on the overall cryptogein structure.

Česky

Elicitiny jsou vylucovány specificky houbami Oomycete genera Pythium a Phytophthora. Biologická funkce elicitinů je stále neznáma.Srovnávali jsme fyzikálně chemické parametry vazby sterol-cryptogen, jejich schopnost vyvolat syntézu aktivního kyslíku,jejich nekrotickou aktivitu v buňkách tabáku.

Návaznosti

GA522/02/0925, projekt VaV

Název: Modelování struktury a interakce bílkovinných elicitorů houby Phytophthora

Investor: Grantová agentura ČR, Modelování struktury a interakce bílkovinných elicitorů houby Phytophthora

Citovat

MIKEŠ, Vladimír, Tomáš KAŠPAROVSKÝ, Jan LOCHMAN, Jiří DAMBORSKY, Michel PONCHET a Jean-Pierre BLEIN. Relationship between secondary structure of elicitins, proteinaceous elicitors of Phytophthora sp. and defense reaction induced in tobacco cells. In Absract Book of 7th Conference of European Foundation for Plant Pathology 2004. 2004. vyd. Aberdeen, UK: EFPP, 2004, s. 10.

@inproceedings{558760,
   author = {Mikeš, Vladimír and Kašparovský, Tomáš and Lochman, Jan and Damborsky, Jiří and Ponchet, Michel and Blein, JeanandPierre},
   address = {Aberdeen, UK},
   booktitle = {Absract Book of 7th Conference of European Foundation for Plant Pathology 2004},
   edition = {2004},
   keywords = {cryptogein; plant defense; Phytophthora},
   language = {eng},
   location = {Aberdeen, UK},
   pages = {10-10},
   publisher = {EFPP},
   title = {Relationship between secondary structure of elicitins, proteinaceous elicitors of Phytophthora sp. and defense reaction induced in tobacco cells.},
   url = {www.efpp.net},
   year = {2004}
}

TY  - JOUR
ID  - 558760
AU  - Mikeš, Vladimír - Kašparovský, Tomáš - Lochman, Jan - Damborsky, Jiří - Ponchet, Michel - Blein, Jean-Pierre
PY  - 2004
TI  - Relationship between secondary structure of elicitins, proteinaceous elicitors of Phytophthora sp. and defense reaction induced in tobacco cells.
PB  - EFPP
CY  - Aberdeen, UK
KW  - cryptogein
KW  - plant defense
KW  - Phytophthora
UR  - www.efpp.net
N2  - Elicitins are secreted specifically by the fungi Oomycete genera Pythium and Phytophthora [1, 2]. Biological function of elicitins is currently unknown. The response is induced by the interaction of elicitins with a putative receptor located on the cytoplasmic membrane. The transfer of signal through the receptor triggers phosphorylation-dephosphorylation cascades in the tobacco resulting in alkalization of the extracellular medium, efflux of potassium and chloride ions, influx of calcium, production of the active species from oxygen [3, 4], changes in composition of the cell wall and the induction of acquired systemic resistance. Binding of sterols to the cavity of cryptogein (elicitin from P. cryptogea) seems to be essential for consecutive association of the cryptogein with a receptor and induction of a biological response in a plant [5]. We prepared a series of mutants of cryptogein, one of the most potent elicitors of this group, with altered capacity of binding sterols (L19-R, I63-F, L15-W, L36-F, M35-F, M35-W, M59-W, M59-F). We compared the physicochemical parameters of sterol-cryptogein binding, their ability to induce the synthesis of active oxygen species, ion fluxes, their necrotic activity on tobacco suspension cells and the ability to induce the expression of pathogen related (PR) proteins in plants. The results showed that some of the early events are proportional to the affinity of cryptogein to bind sterols whereas the others (necrotic effect and the induction of PR protein synthesis) seemed to be dependent on the overall cryptogein structure.
ER  -

MIKEŠ, Vladimír, Tomáš KAŠPAROVSKÝ, Jan LOCHMAN, Jiří DAMBORSKY, Michel PONCHET a Jean-Pierre BLEIN. Relationship between secondary structure of elicitins, proteinaceous elicitors of Phytophthora sp. and defense reaction induced in tobacco cells. In \textit{Absract Book of 7th Conference of European Foundation for Plant Pathology 2004}. 2004. vyd. Aberdeen, UK: EFPP, 2004, s.~10.

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