A balance of apoptosis and proliferation of the satellite cells
in the rat dorsal root ganglia after dorsal rhizotomy

J 2004

A balance of apoptosis and proliferation of the satellite cells in the rat dorsal root ganglia after dorsal rhizotomy

KLUSÁKOVÁ, Ilona a Petr DUBOVÝ

Základní údaje

Originální název

A balance of apoptosis and proliferation of the satellite cells in the rat dorsal root ganglia after dorsal rhizotomy

Název česky

Balance apoptózy a proliferace satelitních buněk ve spinálních gangliích po dorzální rhizotomii

Autoři

KLUSÁKOVÁ, Ilona a Petr DUBOVÝ

Vydání

Journal of Molecular Histology, Kluwer Academic Publishers, 2004, 1567-2379

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/04:00010571

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

TUNEL Ki-67 satellite cells dorsal root ganglion

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 12. 2006 16:53, prof. RNDr. Petr Dubový, CSc.

Anotace

ORIG CZ

V originále

A peripheral axotomy has been shown to cause satellite cell reactions (proliferation, apoptosis, morphological and biochemical changes) in corresponding dorsal root ganglia (DRG). However, only scarce information is available about the reaction of satellite cells to the central axotomy (rhizotomy). Therefore, we have investigated apoptosis and proliferation of the rat DRG cells after dorsal rhizotomy. Thirty six adult rats with dorsal rhizotomy (L1, L2) were left to survival for 2, 4 and 8 weeks and twelve intact rats were used as control. Cryostat sections through axotomized and intact ganglia were incubated for immunohistochemical identification of apoptosis (TUNEL) and proliferation (Ki-67). Satellite cells were identified by immunohistochemical reaction for S-100 protein and their nuclei were counterstained with Hoechst 33342. The sections were digitalized and analyzed using image analysis system (Lucia - G). The neurons were divided into three types according to diameter of their bodies; A (40-75 Ým), B (25-40 Ým) and C (Ź 24.9 Ým). The mean number of satellite cells surrounding each type of neurons was determined. Apoptosis and proliferation were evaluated by means of following criteria: mean percentage of neurons surrounded by TUNEL+ or Ki-67+ satellite cells in individual type of neurons and mean percentage of TUNEL+ or Ki-67+ satellite cells surrounding each type of neurons. No significant changes of total number of satellite cells surrounding individual types of neurons were found up to 8 weeks of survival. The number of TUNEL+ and Ki-67+ satellite cells significantly increased to 4 weeks, but 8 weeks from rhizotomy the positive cells slightly decreased. The largest differences in both increase and decrease of TUNEL+ as well as Ki-67+ satellite cells were detected around C - type of neurons. Simultaneous elevation or diminution of amount of TUNEL+ and Ki-67+ satellite cells following dorsal rhizotomy suggest that both apoptosis and proliferation of the cells are balanced to keep their constant amount around individual types of neurons.

Česky

Periferní axotomie způsobuje reakci satelitních buněk (proliferaci, apoptózu, morfologické a biochemické změny). V práci byly studovány tyto změny po centrální axotomii.

Návaznosti

GA309/03/1199, projekt VaV

Název: Změny v satelitních gliových buňkách a jejich extracelulární matrix po axotomii - podmínky pro tvorbu sympatických kolaterál ve spinálních gangliích ?

Investor: Grantová agentura ČR, Změny v satelitních gliových buňkách a jejich extracelulární matrix po axotomii - podmínky pro tvorbu sympatických kolaterál ve spinálních gangliích ?

Citovat

KLUSÁKOVÁ, Ilona a Petr DUBOVÝ. A balance of apoptosis and proliferation of the satellite cells in the rat dorsal root ganglia after dorsal rhizotomy. Journal of Molecular Histology. Kluwer Academic Publishers, 2004, roč. 35, č. 2, s. 186-186. ISSN 1567-2379.

@article{561597,
   author = {Klusáková, Ilona and Dubový, Petr},
   article_number = {2},
   keywords = {TUNEL Ki-67 satellite cells dorsal root ganglion},
   language = {eng},
   issn = {1567-2379},
   journal = {Journal of Molecular Histology},
   title = {A balance of apoptosis and proliferation of the satellite cells in the rat dorsal root ganglia after dorsal rhizotomy},
   volume = {35},
   year = {2004}
}

TY  - JOUR
ID  - 561597
AU  - Klusáková, Ilona - Dubový, Petr
PY  - 2004
TI  - A balance of apoptosis and proliferation of the satellite cells in the rat dorsal root ganglia after dorsal rhizotomy
JF  - Journal of Molecular Histology
VL  - 35
IS  - 2
SP  - 186-186
EP  - 186-186
PB  - Kluwer Academic Publishers
SN  - 15672379
KW  - TUNEL Ki-67 satellite cells dorsal root ganglion
N2  - A peripheral axotomy has been shown to cause satellite cell reactions (proliferation, apoptosis, morphological and biochemical changes) in corresponding dorsal root ganglia (DRG). However, only scarce information is available about the reaction of satellite cells to the central axotomy (rhizotomy). Therefore, we have investigated apoptosis and proliferation of the rat DRG cells after dorsal rhizotomy. Thirty six adult rats with dorsal rhizotomy (L1, L2) were left to survival for 2, 4 and 8 weeks and twelve intact rats were used as control. Cryostat sections through axotomized and intact ganglia were incubated for immunohistochemical identification of apoptosis (TUNEL) and proliferation (Ki-67). Satellite cells were identified by immunohistochemical reaction for S-100 protein and their nuclei were counterstained with Hoechst 33342. The sections were digitalized and analyzed using image analysis system (Lucia - G). The neurons were divided into three types according to diameter of their bodies; A (40-75 Ým), B (25-40 Ým) and C (Ź 24.9 Ým). The mean number of satellite cells surrounding each type of neurons was determined. Apoptosis and proliferation were evaluated by means of following criteria: mean percentage of neurons surrounded by TUNEL+ or Ki-67+ satellite cells in individual type of neurons and mean percentage of TUNEL+ or Ki-67+ satellite cells surrounding each type of neurons. No significant changes of total number of satellite cells surrounding individual types of neurons were found up to 8 weeks of survival. The number of TUNEL+ and Ki-67+ satellite cells significantly increased to 4 weeks, but 8 weeks from rhizotomy the positive cells slightly decreased. The largest differences in both increase and decrease of TUNEL+ as well as Ki-67+ satellite cells were detected around C - type of neurons. Simultaneous elevation or diminution of amount of TUNEL+ and Ki-67+ satellite cells following dorsal rhizotomy suggest that both apoptosis and proliferation of the cells are balanced to keep their constant amount around individual types of neurons.
ER  -

KLUSÁKOVÁ, Ilona a Petr DUBOVÝ. A balance of apoptosis and proliferation of the satellite cells in the rat dorsal root ganglia after dorsal rhizotomy. \textit{Journal of Molecular Histology}. Kluwer Academic Publishers, 2004, roč.~35, č.~2, s.~186-186. ISSN~1567-2379.

Přehled o publikaci