Characterization of Staphylococcus xylosus strains isolated
from humans clinical material.

a 2004

Characterization of Staphylococcus xylosus strains isolated from humans clinical material.

KUBOŠKOVÁ, Dana, Ivo SEDLÁČEK, Pavel ŠVEC a Petr PETRÁŠ

Základní údaje

Originální název

Characterization of Staphylococcus xylosus strains isolated from humans clinical material.

Název česky

Charakterizace kmenů druhu Staphylococcus xylosus izolovaných z humánního klinického materiálu.

Autoři

KUBOŠKOVÁ, Dana (203 Česká republika, garant), Ivo SEDLÁČEK (203 Česká republika), Pavel ŠVEC (203 Česká republika) a Petr PETRÁŠ (203 Česká republika)

Vydání

14th European Congress of Clinical Microbiology and Infectious Diseases, 2004

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.361

Kód RIV

RIV/00216224:14310/04:00028916

Organizační jednotka

Přírodovědecká fakulta

ISSN

Klíčová slova anglicky

Identification; Staphylococcus; Human clinical material

Štítky

Human clinical material, identification, Staphylococcus

Změněno: 31. 3. 2010 14:06, prof. RNDr. Ivo Sedláček, CSc.

Anotace

V originále

Objectives: Staphylococci are common inhabitants of skin, skin glands and mucous membranes of mammals and birds. Certain Staphylococcus species are found as aetiological agents of a variety of human and animal infections as well. S. xylosus and S. equorum represents two phylogenetically and biochemically close species. Although S. xylosus is usually only transient on humans and is primarily acquired from domestic animals and their products it has been isolated from clinical material, mainly from urinary-tract infections. It is difficult to distinguish S. xylosus and S. equorum on the base of the biotyping. S. equorum was isolated from animal sources, but it has not been isolated from human clinical specimens yet. Methods: A group of 18 presumptive S. xylosus strains isolated from human clinical specimens and reference strains (S. xylosus, S. equorum and S. gallinarum) were analysed by biochemical tests and ribotyping. Biochemical properties were tested by API Staph and ID 32 Staph kits and by conventional tests. Ribotyping was done with EcoRI and HindIII restriction enzymes and a probe complementary to 16S and 23S rRNA from E. coli. Results: Phenotypic data of key tests corresponded with species description of S. xylosus except for one intermediate strain S. xylosus/S. equorum. Some results obtained for acid production were different from S. xylosus description, but identification to the species level was acceptable. Ribotyping with EcoRI divided tested strains into two groups, the S. xylosus group and smaller group which, as we supposed, belongs to the S. equorum species. This group contained of two reference strains and four clinical isolates. Group S. equorum was clearly distinguished also with HindIII, but S. xylosus group was divided into three smaller groups by using HindIII. Results of ribotype profiles did not correspond with biochemical characterisation of the isolates. Conclusions: There were identified 17 S. xylosus strains and one intermediate strain S. xylosus/S. equorum by biochemical tests. Contrary to biotyping, the colony size on the P agar and the ribotyping with both used restriction enzymes, showed, that four strains from tested series represent S. equorum species. This is the first case of occurrence S. equorum in human clinical specimens. Ribotyping with EcoRI and with HindIII showed heterogeneous ribotype profiles. These results imply that this method could be suitable for intraspecies characterisation of S. xylosus and S. equorum.

Návaznosti

GA301/02/1505, projekt VaV

Název: Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků

Investor: Grantová agentura ČR, Molekulární diagnostika, epidemiologie a klasifikace klinicky významných grampozitivních koků

Citovat

KUBOŠKOVÁ, Dana, Ivo SEDLÁČEK, Pavel ŠVEC a Petr PETRÁŠ. Characterization of Staphylococcus xylosus strains isolated from humans clinical material. In 14th European Congress of Clinical Microbiology and Infectious Diseases. 2004. ISSN 1198-743X.

@proceedings{563513,
   author = {Kubošková, Dana and Sedláček, Ivo and Švec, Pavel and Petráš, Petr},
   booktitle = {14th European Congress of Clinical Microbiology and Infectious Diseases},
   keywords = {Identification; Staphylococcus; Human clinical material},
   language = {eng},
   title = {Characterization of Staphylococcus xylosus strains isolated from humans clinical material.},
   year = {2004}
}

TY  - CONF
ID  - 563513
AU  - Kubošková, Dana - Sedláček, Ivo - Švec, Pavel - Petráš, Petr
PY  - 2004
TI  - Characterization of Staphylococcus xylosus strains isolated from humans clinical material.
KW  - Identification
KW  - Staphylococcus
KW  - Human clinical material
N2  - Objectives: Staphylococci are common inhabitants of skin, skin glands and mucous membranes of mammals and birds. Certain Staphylococcus species are found as aetiological agents of a variety of human and animal infections as well. S. xylosus and S. equorum represents two phylogenetically and biochemically close species. Although S. xylosus is usually only transient on humans and is primarily acquired from domestic animals and their products it has been isolated from clinical material, mainly from urinary-tract infections. It is difficult to distinguish S. xylosus and S. equorum on the base of the biotyping. S. equorum was isolated from animal sources, but it has not been isolated from human clinical specimens yet. Methods: A group of 18 presumptive S. xylosus strains isolated from human clinical specimens and reference strains (S. xylosus, S. equorum and S. gallinarum) were analysed by biochemical tests and ribotyping. Biochemical properties were tested by API Staph and ID 32 Staph kits and by conventional tests. Ribotyping was done with EcoRI and HindIII restriction enzymes and a probe complementary to 16S and 23S rRNA from E. coli. Results: Phenotypic data of key tests corresponded with species description of S. xylosus except for one intermediate strain S. xylosus/S. equorum. Some results obtained for acid production were different from S. xylosus description, but identification to the species level was acceptable. Ribotyping with EcoRI divided tested strains into two groups, the S. xylosus group and smaller group which, as we supposed, belongs to the S. equorum species. This group contained of two reference strains and four clinical isolates. Group S. equorum was clearly distinguished also with HindIII, but S. xylosus group was divided into three smaller groups by using HindIII. Results of ribotype profiles did not correspond with biochemical characterisation of the isolates. Conclusions: There were identified 17 S. xylosus strains and one intermediate strain S. xylosus/S. equorum by biochemical tests. Contrary to biotyping, the colony size on the P agar and the ribotyping with both used restriction enzymes, showed, that four strains from tested series represent S. equorum species. This is the first case of occurrence S. equorum in human clinical specimens. Ribotyping with EcoRI and with HindIII showed heterogeneous ribotype profiles. These results imply that this method could be suitable for intraspecies characterisation of S. xylosus and S. equorum.
ER  -

KUBOŠKOVÁ, Dana, Ivo SEDLÁČEK, Pavel ŠVEC a Petr PETRÁŠ. Characterization of Staphylococcus xylosus strains isolated from humans clinical material. In \textit{14th European Congress of Clinical Microbiology and Infectious Diseases}. 2004. ISSN~1198-743X.

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