2004
Dynamics of HP1 transgene loci movement and silencing in living cells
ONDŘEJ, Vladan, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Pavel MATULA, Petr MATULA et. al.Základní údaje
Originální název
Dynamics of HP1 transgene loci movement and silencing in living cells
Název česky
Dynamika pohybu a klidového stavu HP1 transgenních lokusů v živých buňkách
Autoři
ONDŘEJ, Vladan (203 Česká republika), Stanislav KOZUBEK (203 Česká republika, garant), Emilie LUKÁŠOVÁ (203 Česká republika), Pavel MATULA (203 Česká republika), Petr MATULA (203 Česká republika) a Michal KOZUBEK (203 Česká republika)
Vydání
First Edition 2004. Brno, Biophysics of the Genome, od s. 66-71, 6 s. 2004
Nakladatel
Masaryk University
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
Genetika a molekulární biologie
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14330/04:00009705
Organizační jednotka
Fakulta informatiky
ISBN
80-210-3560-9
Klíčová slova anglicky
cytometry
Štítky
Příznaky
Mezinárodní význam
Změněno: 6. 8. 2010 14:58, doc. RNDr. Petr Matula, Ph.D.
V originále
Intranuclear localization and mobility of Cy3 labelled transgene loci were studied in living cells. Observations showed occurrence of several transgene copies in each cell nucleus. The majority of copies were localized in the centromeric heterochromatin defined by HP1beta-GFP fusion protein, the minority of copies in euchromatin. The tracking of loci showed restricted diffusive motion of these in the short-time range. During long-time observation of HP1beta domains and transgene loci, we have found that in most cases the proximity of these objects decreased within time. The changes of positions of HP1 domains were very small but transgene loci displayed directional movement towards the relevant HP1 domain. Our data records support the idea that the cell nucleus consists of several separated higher-order compartments. The genes relocate within these compartments, which is finally connected with changes of their expression status. In our experiments, the transgene loci were strongly silenced. Three days after the transfection of cells, their expression was reduced dramatically. At that time we found colocalization of transgene loci with HP1beta domains and centromeres. Moreover, the loci were methylated, which was confirmed using antibodies against trimethylated histone H3 at Lys9.
Česky
Dynamika pohybu a klidového stavu HP1 transgenních lokusů v živých buňkách.
Návaznosti
| AV0Z5004920, záměr |
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| IAA1065203, projekt VaV |
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| IAA5004306, projekt VaV |
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| IBS5004010, projekt VaV |
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| 1A8241, projekt VaV |
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