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@article{564526,
author = {Halámek, Jan and Makower, Alexander and Knosche, Kristina and Skládal, Petr and Scheller, Frieder},
article_location = {The Netherlands},
article_number = {2},
keywords = {piezoelectric sensory; enzyme inhibition;},
language = {eng},
issn = {0039-9140},
journal = {Talanta},
title = {Piezoelectric affinity sensors for cocaine and cholinesterase inhibitors},
url = {http://dx.doi.org/10.1016/j.talanta.2004.07.008},
volume = {65},
year = {2005}
}
TY - JOUR
ID - 564526
AU - Halámek, Jan - Makower, Alexander - Knosche, Kristina - Skládal, Petr - Scheller, Frieder
PY - 2005
TI - Piezoelectric affinity sensors for cocaine and cholinesterase inhibitors
JF - Talanta
VL - 65
IS - 2
SP - 337-342
EP - 337-342
PB - Elsevier Science
SN - 00399140
KW - piezoelectric sensory
KW - enzyme inhibition;
UR - http://dx.doi.org/10.1016/j.talanta.2004.07.008
N2 - We report here the development of piezoelectric affinity sensors for cocaine and cholinesterase inhibitors based on the formation of affinity complexes between an immobilized cocaine derivative and an anti-cocaine antibody or cholinesterase. For both binding reactions benzoylecgonine-1,8-diamino-3,4-dioxaoctane (BZE-DADOO) was immobilized on the surface of the sensor. For immobilization, pre-conjugated BZE-DADOO with 11-mercaptomonoundecanoic acid (MUA) via 2-(5-norbornen-2,3-dicarboximide)-1,1,3,3-tetramethyluronium-tetrafluoroborate (TNTU) allowed the formation of a chemisorbed monolayer on the piezosensor surface. The detection of cocaine was based on a competitive assay. The change of frequency measured after 300 s of the binding reaction was used as the signal. The maximum binding of the antibody resulted in a frequency decrease of 35 Hz (with an imprecision 3%, n = 3) while the presence of 100 pmol l1 cocaine decreased the binding by 11%. The limit of detection was consequently below 100 pmol l1 for cocaine. The total time of one analysis was 15 min. This BZE-DADOO-modified sensor was adapted for the detection of organophosphates. BZE-DADOO a competitive inhibitor served as binding element for cholinesterase in a competitive assay.
ER -
HALÁMEK, Jan, Alexander MAKOWER, Kristina KNOSCHE, Petr SKLÁDAL a Frieder SCHELLER. Piezoelectric affinity sensors for cocaine and cholinesterase inhibitors. \textit{Talanta}. The Netherlands: Elsevier Science, 2005, roč.~65, č.~2, s.~337-342. ISSN~0039-9140.
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