Methylation of histones in myeloid leukemias as a potential
marker of granulocyte abnormalities

J 2005

Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities

LUKÁŠOVÁ, Emilie, Zdeněk KOŘÍSTEK, Martin FALK, Stanislav KOZUBEK, Sergei GRIGORYEV et. al.

Basic information

Original name

Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities

Name in Czech

Metylace histonů u myeloidních leukémií jako potenciální znak abnormality granulocytů

Authors

LUKÁŠOVÁ, Emilie (203 Czech Republic), Zdeněk KOŘÍSTEK (203 Czech Republic, belonging to the institution), Martin FALK (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Sergei GRIGORYEV (643 Russian Federation), Michal KOZUBEK (203 Czech Republic, guarantor, belonging to the institution), Vladan ONDŘEJ (203 Czech Republic) and Iva KROUPOVÁ (203 Czech Republic)

Edition

Journal of Leukocyte Biology, New York, Society for Leukocyte Biology, 2005, 0741-5400

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.627

RIV identification code

RIV/00216224:14330/05:00012357

Organization unit

Faculty of Informatics

UT WoS

000227390800013

Keywords in English

human granulocytes differentiation; chromatin condensation; heterochromatin; HP1 proteins

Abstract

ORIG CZ

V originále

We show that common heterochromatin antigenic protein markers [HP1alpha, -betta, -gamma and mono-, di-, and trimethylated histone H3 lysine 9 (H3K9)], although present in human blood progenitor CD34+ cells, differentiated lymphocytes, and monocytes, are absent in neutrophil granulocytes and to large extent, in eosinophils. Monomethylated and in particular, dimethylated H3K9 are present to variable degrees in the granulocytes of chronic myeloid leukemia (CML) patients, without being accompanied by HP1 proteins. In patients with an acute phase of CML and in acute myeloid leukemia patients, strong methylation of H3K9 and all isoforms of HP1 are detected. In chronic forms of CML, no strong correlations among the level of histone methylation, disease progression, and modality of treatment were observed. Histone methylation was found even in "cured" patients without Philadelphia chromosome (Ph) resulting from +(9;22)(q34;q11) BCR/ABL translocation, suggesting an incomplete process of developmentally regulated chromatin remodeling in the granulocytes of these patients. Similarly, reprogramming of leukemia HL-60 cells to terminal differentiation by retinoic acid does not eliminate H3K9 methylation and the presence of HP1 isoforms from differentiated granulocytes. Thus, our study shows for the first time that histone H3 methylation may be changed dramatically during normal cell differentiation. The residual histone H3 methylation in myeloid leukemia cells suggests an incomplete chromatin condensation that may be linked to the leukemia cell proliferation and may be important for the prognosis of disease treatment and relapse.

In Czech

Metylace histonů u myeloidních leukémií jako potenciální znak abnormality granulocytů.

Links

NC6987, research and development project

Name: Epigeneticky kontrolované změny exprese genů u nádorových onemocnění

Investor: Ministry of Health of the CR, Epigenetic control of gene expression in malignant diseases

Citovat

LUKÁŠOVÁ, Emilie, Zdeněk KOŘÍSTEK, Martin FALK, Stanislav KOZUBEK, Sergei GRIGORYEV, Michal KOZUBEK, Vladan ONDŘEJ and Iva KROUPOVÁ. Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities. Journal of Leukocyte Biology. New York: Society for Leukocyte Biology, 2005, vol. 77, No 1, p. 100-111. ISSN 0741-5400.

@article{564541,
   author = {Lukášová, Emilie and Kořístek, Zdeněk and Falk, Martin and Kozubek, Stanislav and Grigoryev, Sergei and Kozubek, Michal and Ondřej, Vladan and Kroupová, Iva},
   article_location = {New York},
   article_number = {1},
   keywords = {human granulocytes differentiation; chromatin condensation; heterochromatin; HP1 proteins},
   language = {eng},
   issn = {0741-5400},
   journal = {Journal of Leukocyte Biology},
   title = {Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities},
   volume = {77},
   year = {2005}
}

TY  - JOUR
ID  - 564541
AU  - Lukášová, Emilie - Kořístek, Zdeněk - Falk, Martin - Kozubek, Stanislav - Grigoryev, Sergei - Kozubek, Michal - Ondřej, Vladan - Kroupová, Iva
PY  - 2005
TI  - Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities
JF  - Journal of Leukocyte Biology
VL  - 77
IS  - 1
SP  - 100-111
EP  - 100-111
PB  - Society for Leukocyte Biology
SN  - 07415400
KW  - human granulocytes differentiation
KW  - chromatin condensation
KW  - heterochromatin
KW  - HP1 proteins
N2  - We show that common heterochromatin antigenic protein markers [HP1alpha, -betta, -gamma and mono-, di-, and trimethylated histone H3 lysine 9 (H3K9)], although present in human blood progenitor CD34+ cells, differentiated lymphocytes, and monocytes, are absent in neutrophil granulocytes and to large extent, in eosinophils. Monomethylated and in particular, dimethylated H3K9 are present to variable degrees in the granulocytes of chronic myeloid leukemia (CML) patients, without being accompanied by HP1 proteins. In patients with an acute phase of CML and in acute myeloid leukemia patients, strong methylation of H3K9 and all isoforms of HP1 are detected. In chronic forms of CML, no strong correlations among the level of histone methylation, disease progression, and modality of treatment were observed. Histone methylation was found even in "cured" patients without Philadelphia chromosome (Ph) resulting from +(9;22)(q34;q11) BCR/ABL translocation, suggesting an incomplete process of developmentally regulated chromatin remodeling in the granulocytes of these patients. Similarly, reprogramming of leukemia HL-60 cells to terminal differentiation by retinoic acid does not eliminate H3K9 methylation and the presence of HP1 isoforms from differentiated granulocytes. Thus, our study shows for the first time that histone H3 methylation may be changed dramatically during normal cell differentiation. The residual histone H3 methylation in myeloid leukemia cells suggests an incomplete chromatin condensation that may be linked to the leukemia cell proliferation and may be important for the prognosis of disease treatment and relapse.
ER  -

LUKÁŠOVÁ, Emilie, Zdeněk KOŘÍSTEK, Martin FALK, Stanislav KOZUBEK, Sergei GRIGORYEV, Michal KOZUBEK, Vladan ONDŘEJ and Iva KROUPOVÁ. Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities. \textit{Journal of Leukocyte Biology}. New York: Society for Leukocyte Biology, 2005, vol.~77, No~1, p.~100-111. ISSN~0741-5400.

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