Lignans in embryogenic culture of Schisandra chinensis

D 2004

Lignans in embryogenic culture of Schisandra chinensis

VLAŠÍNOVÁ, Helena; Alexandra SMÍŠKOVÁ; Jiří SLANINA; Eva TÁBORSKÁ; L. HAVEL et al.

Základní údaje

Originální název

Lignans in embryogenic culture of Schisandra chinensis

Název česky

Lignany v embyogenní kultuře Schisandra chinensis

Autoři

VLAŠÍNOVÁ, Helena; Alexandra SMÍŠKOVÁ; Jiří SLANINA; Eva TÁBORSKÁ a L. HAVEL

Vydání

1. vyd. Bratislava, X. Dny rostlinné fyziologie, s. 38-38, 2004

Nakladatel

Společnost fyziologie rostlin

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

40100 4.1 Agriculture, Forestry, and Fisheries

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/04:00011937

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Schisandra chinensis; lignans; embryogenic culture

Štítky

embryogenic culture, lignans, Schisandra chinensis

Změněno: 24. 1. 2005 08:54, Mgr. Jiří Slanina, Ph.D.

Anotace

ORIG CZ

V originále

Dibenzo[a,c]cyclooctadiene lignans are unique natural substances, found mainly in family Schisandraceae, the oldest family of dicotyledonous plants. Schisandra chinensis is well-known medicinal plants of traditional Chinese medicine particularly as tonic and antitusic. Their lignans have been shown to possess a broad range of biological effects, including antiviral, antioxidative, hepatoprotective and anticancer activities (Chen et al. 1997, Opletal et al. 2001). Commercial vegetative and seed propagation is limited. Introduction of micropropagation of selected plants via somatic embryogenesis would therefore contribute to rapid distribution of valuable genotypes. S. chinensis calluses, derived from endosperm and immature zygotic embryo were developed on MS and VW5 medium. Thidiazuron was tested as an inductor of somatic embryogenesis and compared with 2,4-dichlorophenoxyacetic acid. Sustainability and morphological features of cultures depended on induction and maintenance regimes. Partially synchronous embryogenic system was established on induction medium containing thidiazuron. 2,4-dichlorphenoxyacetic acid and benzylaminopurine proved essential for the maintenance and abscisic acid and polyethylene glycol 4000 supplement for development and maturation of somatic embryos. After conversion, emblings were hardened in Climacell box and then cultured in a greenhouse. High performance liquid chromatography method on reversed phase was applied for profiling of lignans in various stages of the culture and as a potential tool for further selection and breeding. The highest amount (three times lower than in leaves of mother plant) was found in mature embryos, where the dominant substance was deoxyschizandrin. Lignans were detected in all stages of embryo development as a potential tool for further breeding. Content of lignans found in fruits (1.6 %) was higher than that in pericarps (0.31 %), leaves (0.2 %) and in vitro cultures (max. 0.047 %). However, using of plant cell culture for the production of lignans seems to be one of the methods capable to improve yields of individual lignans. The main lignan in samples of cell cultures was deoxyschizandrin, its maximum yield reached 0.13 % based on dry weight. Its concentration was 10 times lower than that in fruits and 3 times lower than that in leaves. Gomisin N, which was found more cytotoxic against HeLa cells than other lignans showed increased level after addition of polyamine putrescin into the cultivation medium. The determination of lignans by improved HPLC method is susceptible for selection of the best lines and theirs further breeding.

Česky

Lignany v embyogenní kultuře Schisandra chinensis byly stanoveny.

Návaznosti

GA521/02/1129, projekt VaV

Název: Využití biotechnologických metod k produkci sekundárních metabolitů, šlechtění a mikropropagaci léčivé rostliny klanoprašky čínské

Investor: Grantová agentura ČR, Využití biotechnologických metod k produkci sekundárních metabolitů, šlechtění a mikropropagaci léčivé rostliny klanoprašky čínské

Citovat

VLAŠÍNOVÁ, Helena; Alexandra SMÍŠKOVÁ; Jiří SLANINA; Eva TÁBORSKÁ a L. HAVEL. Lignans in embryogenic culture of Schisandra chinensis. In X. Dny rostlinné fyziologie. 1. vyd. Bratislava: Společnost fyziologie rostlin, 2004, s. 38.

@inproceedings{564871,
   author = {Vlašínová, Helena and Smíšková, Alexandra and Slanina, Jiří and Táborská, Eva and Havel, L.},
   address = {Bratislava},
   booktitle = {X. Dny rostlinné fyziologie},
   edition = {1.},
   keywords = {Schisandra chinensis; lignans; embryogenic culture},
   language = {eng},
   location = {Bratislava},
   pages = {38-38},
   publisher = {Společnost fyziologie rostlin},
   title = {Lignans in embryogenic culture of Schisandra chinensis},
   year = {2004}
}

TY  - CONF
ID  - 564871
AU  - Vlašínová, Helena - Smíšková, Alexandra - Slanina, Jiří - Táborská, Eva - Havel, L.
PY  - 2004
TI  - Lignans in embryogenic culture of Schisandra chinensis
PB  - Společnost fyziologie rostlin
CY  - Bratislava
KW  - Schisandra chinensis
KW  - lignans
KW  - embryogenic culture
N2  - Dibenzo[a,c]cyclooctadiene lignans are unique natural substances, found mainly in family Schisandraceae, the oldest family of dicotyledonous plants. Schisandra chinensis is well-known medicinal plants of traditional Chinese medicine particularly as tonic and antitusic. Their lignans have been shown to possess a broad range of biological effects, including antiviral, antioxidative, hepatoprotective and anticancer activities (Chen et al. 1997, Opletal et al. 2001). Commercial vegetative and seed propagation is limited. Introduction of micropropagation of selected plants via somatic embryogenesis would therefore contribute to rapid distribution of valuable genotypes. S. chinensis calluses, derived from endosperm and immature zygotic embryo were developed on MS and VW5 medium. Thidiazuron was tested as an inductor of somatic embryogenesis and compared with 2,4-dichlorophenoxyacetic acid. Sustainability and morphological features of cultures depended on induction and maintenance regimes. Partially synchronous embryogenic system was established on induction medium containing thidiazuron. 2,4-dichlorphenoxyacetic acid and benzylaminopurine proved essential for the maintenance and abscisic acid and polyethylene glycol 4000 supplement for development and maturation of somatic embryos. After conversion, emblings were hardened in Climacell box and then cultured in a greenhouse. High performance liquid chromatography method on reversed phase was applied for profiling of lignans in various stages of the culture and as a potential tool for further selection and breeding. The highest amount (three times lower than in leaves of mother plant) was found in mature embryos, where the dominant substance was deoxyschizandrin. Lignans were detected in all stages of embryo development as a potential tool for further breeding. Content of lignans found in fruits (1.6 %) was higher than that in pericarps (0.31 %), leaves (0.2 %) and in vitro cultures (max. 0.047 %). However, using of plant cell culture for the production of lignans seems to be one of the methods capable to improve yields of individual lignans. The main lignan in samples of cell cultures was deoxyschizandrin, its maximum yield reached 0.13 % based on dry weight. Its concentration was 10 times lower than that in fruits and 3 times lower than that in leaves. Gomisin N, which was found more cytotoxic against HeLa cells than other lignans showed increased level after addition of polyamine putrescin into the cultivation medium. The determination of lignans by improved HPLC method is susceptible for selection of the best lines and theirs further breeding.
ER  -

VLAŠÍNOVÁ, Helena; Alexandra SMÍŠKOVÁ; Jiří SLANINA; Eva TÁBORSKÁ a L. HAVEL. Lignans in embryogenic culture of Schisandra chinensis. In \textit{X. Dny rostlinné fyziologie}. 1. vyd. Bratislava: Společnost fyziologie rostlin, 2004, s.~38.

Přehled o publikaci