Přímý průkaz Treponema pallidum v diagnostice syfilis

WOZNICOVÁ, Vladana. Přímý průkaz Treponema pallidum v diagnostice syfilis. Epidemiologie, mikrobiologie, imunologie. Praha: ČLS JEP, 2004, roč. 53, č. 3, s. 121-125. ISSN 1210-7913.

Základní údaje

Originální název

Přímý průkaz Treponema pallidum v diagnostice syfilis

Název anglicky

Direct detection of Treponema pallidum in diagnosis of syphilis

Autoři

WOZNICOVÁ, Vladana (203 Česká republika, garant)

Vydání

Epidemiologie, mikrobiologie, imunologie, Praha, ČLS JEP, 2004, 1210-7913

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Další údaje

Jazyk

čeština

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/04:00030904

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

syphilis; Treponema pallidum; direct detection; polA PCR

Štítky

direct detection, polA PCR, syphilis, Treponema pallidum

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Anotace

V originále

Available methods for direct diagnosis of syphilis are summarized with emphasis being on those promising for routine use. Direct detection of the causative agent T. pallidum is limited since the agent is not able to synthesize enzyme cofactors, fatty acids and nucleotides de novo, is completely dependent on its host and thus culture on synthetic media is not feasible. Direct diagnosis of syphilis is based on rabbit infectivity testing (RIT), dark field or fluorescent microscopy and recently also on molecular biological methods used with increasing frequency in routine practice. Suitability and usability of different methods for direct detection of T. pallidum at different stages of syphilis are explained. Except for molecular biological methods, most of detection techniques can only be used at the primary and secondary stages or in early congenital syphilis. Major PCR methods for diagnosis of syphilis are presented. Not all of them are suitable for use in routine practice owing to differences in their sensitivity and design. The polA PCR method appears to be the most promising in this regard.

Anglicky

Available methods for direct diagnosis of syphilis are summarized with emphasis being on those promising for routine use. Direct detection of the causative agent T. pallidum is limited since the agent is not able to synthesize enzyme cofactors, fatty acids and nucleotides de novo, is completely dependent on its host and thus culture on synthetic media is not feasible. Direct diagnosis of syphilis is based on rabbit infectivity testing (RIT), dark field or fluorescent microscopy and recently also on molecular biological methods used with increasing frequency in routine practice. Suitability and usability of different methods for direct detection of T. pallidum at different stages of syphilis are explained. Except for molecular biological methods, most of detection techniques can only be used at the primary and secondary stages or in early congenital syphilis. Major PCR methods for diagnosis of syphilis are presented. Not all of them are suitable for use in routine practice owing to differences in their sensitivity and design. The polA PCR method appears to be the most promising in this regard.