Sub-picomole high-performance liquid chromatographic/mass
spectrometric determination of glutatione in the maize (Zea
mays L.) kernels exposed to cadmium

J 2004

Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutatione in the maize (Zea mays L.) kernels exposed to cadmium

KLEJDUS, Boivoj; Josef ZEHNÁLEK; Vojtch ADAM; Jií PETEK; René KIZEK et al.

Základní údaje

Originální název

Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutatione in the maize (Zea mays L.) kernels exposed to cadmium

Název česky

Využití vysokoúinné kapalinové chromatografie pro stanovení glutatiónu v kukuiných jádrech vystavených kadmiu

Autoři

KLEJDUS, Boivoj; Josef ZEHNÁLEK; Vojtch ADAM; Jií PETEK; René KIZEK; Jan VACEK; Libuše TRNKOVÁ; Roland ROZIK; Ladislav HAVEL a Vlastimil KUBÁ

Vydání

Analytica Chimica Acta, elsevier, 2004, 0003-2670

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.588

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/04:00011228

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000223567500013

Klíčová slova anglicky

Maize; Cadmium; Glutathione

Štítky

cadmium, Glutathione, maize

Změněno: 20. 6. 2008 12:07, prof. RNDr. Libuše Trnková, CSc.

Anotace

ORIG CZ

V originále

Changes in fresh weight, total protein amounts (Bradfords method), cadmium concentration (DPASV) and glutathione content (HPLC/MS) were studied in maize kernels cultivated for 5 days at three different cadmium concentrations (0, 10 and 100 micromol /l CdCl2). A highly sensitive HPLC/MS method was used for the determination of glutathione on a reversed-phase Atlantis dC18 chromatographic column. An isocratic mode with acetonitrile0.01% TFA (5:95, flow rate 0.1 ml min1 and 30 C) was applied.The m/z spectra and the data for the selected ion monitoring (SIM) mode were recorded at m/z for glutathione 308 - 179. Cadmium concentration was measured by a differential pulse adsorptive stripping voltammetry (DPASV) after deposition on a hanging mercury drop electrode (HMDE) at potential 0.7V (accumulation time 180 s, acetate buffer of pH 3.6, 22 C). An AUTOLAB with a VA-Stand 663 and a three-electrode system consisting of the HMDE as a working electrode, an Ag/AgCl/3 mol l1 KCl as a reference electrode and a Pt-wire as an auxiliary electrode was employed. The maize kernels exposed to the highest cadmium concentration (100 micromol/l)germinated formerly and much better. A rapid increase of the fresh weight probably relates with more intensive uptake of water in order to decrease cadmium concentration. An intensive preservation of homeostasis of Cd2+ ions in the germinating plants by defending mechanisms might explain differences of uptake rate of cadmium. The linear increase of GSH content with the exposure time at all studied concentration suggests the defending mechanisms might be triggered by concentrations of a heavy metal.

Česky

Využití vysokoúinné kapalinové chromatografie ve spojení s hmotnostní spektrometrií pro stanovení glutatiónu v kukuiných jádrech vystavených kadmiu

Návaznosti

GA203/02/0422, projekt VaV

Název: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

Investor: Grantová agentura ČR, Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

IAA1163201, projekt VaV

Název: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Investor: Akademie věd ČR, Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Citovat

KLEJDUS, Boivoj; Josef ZEHNÁLEK; Vojtch ADAM; Jií PETEK; René KIZEK; Jan VACEK; Libuše TRNKOVÁ; Roland ROZIK; Ladislav HAVEL a Vlastimil KUBÁ. Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutatione in the maize (Zea mays L.) kernels exposed to cadmium. Analytica Chimica Acta. elsevier, 2004, roč. 520/2004, 1-2, s. 117-124. ISSN 0003-2670.

@article{567302,
   author = {Klejdus, Boivoj and Zehnálek, Josef and Adam, Vojtch and Petek, Jií and Kizek, René and Vacek, Jan and Trnková, Libuše and Rozik, Roland and Havel, Ladislav and Kubá, Vlastimil},
   article_number = {1-2},
   keywords = {Maize; Cadmium; Glutathione},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutatione in the maize (Zea mays L.) kernels exposed to cadmium},
   volume = {520/2004},
   year = {2004}
}

TY  - JOUR
ID  - 567302
AU  - Klejdus, Boivoj - Zehnálek, Josef - Adam, Vojtch - Petek, Jií - Kizek, René - Vacek, Jan - Trnková, Libuše - Rozik, Roland - Havel, Ladislav - Kubá, Vlastimil
PY  - 2004
TI  - Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutatione in the maize (Zea mays L.) kernels exposed to cadmium
JF  - Analytica Chimica Acta
VL  - 520/2004
IS  - 1-2
SP  - 117-124
EP  - 117-124
PB  - elsevier
SN  - 00032670
KW  - Maize
KW  - Cadmium
KW  - Glutathione
N2  - Changes in fresh weight, total protein amounts (Bradfords method), cadmium concentration (DPASV) and glutathione content (HPLC/MS) were studied in maize kernels cultivated for 5 days at three different cadmium concentrations (0, 10 and 100 micromol /l CdCl2). A highly sensitive HPLC/MS method was used for the determination of glutathione on a reversed-phase Atlantis dC18 chromatographic column. An isocratic mode with acetonitrile0.01% TFA (5:95, flow rate 0.1 ml min1 and 30 C) was applied.The m/z spectra and the data for the selected ion monitoring (SIM) mode were recorded at m/z for glutathione 308 - 179. Cadmium concentration was measured by a differential pulse adsorptive stripping voltammetry (DPASV) after deposition on a hanging mercury drop electrode (HMDE) at potential 0.7V (accumulation time 180 s, acetate buffer of pH 3.6, 22 C). An AUTOLAB with a VA-Stand 663 and a three-electrode system consisting of the HMDE as a working electrode, an Ag/AgCl/3 mol l1 KCl as a reference electrode and a Pt-wire as an auxiliary electrode was employed. The maize kernels exposed to the highest cadmium concentration (100 micromol/l)germinated formerly and much better. A rapid increase of the fresh weight probably relates with more intensive uptake of water in order to decrease cadmium concentration. An intensive preservation of homeostasis of Cd2+ ions in the germinating plants by defending mechanisms might explain differences of uptake rate of cadmium. The linear increase of GSH content with the exposure time at all studied concentration suggests the defending mechanisms might be triggered by concentrations of a heavy metal.
ER  -

KLEJDUS, Boivoj; Josef ZEHNÁLEK; Vojtch ADAM; Jií PETEK; René KIZEK; Jan VACEK; Libuše TRNKOVÁ; Roland ROZIK; Ladislav HAVEL a Vlastimil KUBÁ. Sub-picomole high-performance liquid chromatographic/mass spectrometric determination of glutatione in the maize (Zea mays L.) kernels exposed to cadmium. \textit{Analytica Chimica Acta}. elsevier, 2004, roč.~520/2004, 1-2, s.~117-124. ISSN~0003-2670.

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