Electrochemical determination of cysteine-containing 
biomolecules by means of catalytic reactions on a mercury
electrode

D 2004

Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode

VACEK, Jan, Libuše TRNKOVÁ, František JELEN a René KIZEK

Základní údaje

Originální název

Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode

Název česky

Elektrochemické stanovení biomolekul obsahujících cystein pomocí katalytických reakcí na rtuové elektrod

Autoři

VACEK, Jan (203 Česká republika), Libuše TRNKOVÁ (203 Česká republika, garant), František JELEN (203 Česká republika) a René KIZEK (203 Česká republika)

Vydání

1. vyd. Thessaloniki, Greece, Book of Abstracts - 55th Annual Meeting of the ISE, od s. 431 - 431, 1 s. 2004

Nakladatel

International Society of Electrochemistry

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10405 Electrochemistry

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/04:00011235

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

metallothioneines; phytochelatins; glutathione; mercury electrode;catalytic reactions; Brdicka reaction;peak H

Štítky

Brdicka reaction, catalytic reactions, Glutathione, Mercury Electrode, metallothioneines, Peak H, phytochelatins

Změněno: 15. 2. 2005 21:22, prof. RNDr. Libuše Trnková, CSc.

Anotace

ORIG CZ

V originále

The group cysteine-containing peptides (metallothioneines, phytochelatins, and glutathione) plays an important role in active homeostasis and detoxification of metals in animal, plant, and/or microorganism cells. In this study we describe a novel approach to electroanalysis of cys-containing peptides and proteins. This approach is based on catalytic processes (presodium wave, peak H, Brdicka current) proceeding at negative potentials (ca. 1.8 V, Ag/AgCl/3M KCl) on the hanging mercury drop electrode (HMDE). A presodium wave is current-potential response accompanied with catalytic hydrogen evolution from aqueous solutions in the presence of proteins. It was found that the sulfhydryl(SH) groups in proteins are responsible for presodium catalytic processes. The highest sensitivity in the determination was observed with derivative chronopotentiometric stripping analysis (CPSA), which monitored the catalytic signal known as peak H. The determination of the low content of Cys-peptides was successfully performed in a cobalt(III)-containing solution with ammonia buffer by differential pulse voltammetry (DPV). The CPSA (peak H) and DPV (Brdicka reaction) can be used in connection with the adsorptive transfer stripping (AdTS) technique, which is capable of determining nano/femtomole levels of metallothioneine, phytochelatin and glutathione in mL of the sample.

Česky

Elektrochemické stanovení biomolekul obsahujících cystein pomocí katalytických reakcí na rtuové elektrod.

Návaznosti

GA203/02/0422, projekt VaV

Název: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

Investor: Grantová agentura ČR, Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

IAA1163201, projekt VaV

Název: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Investor: Akademie věd ČR, Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Citovat

VACEK, Jan, Libuše TRNKOVÁ, František JELEN a René KIZEK. Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode. In Book of Abstracts - 55th Annual Meeting of the ISE. 1. vyd. Thessaloniki, Greece: International Society of Electrochemistry, 2004, s. 431 - 431.

@inproceedings{567329,
   author = {Vacek, Jan and Trnková, Libuše and Jelen, František and Kizek, René},
   address = {Thessaloniki, Greece},
   booktitle = {Book of Abstracts - 55th Annual Meeting of the ISE},
   edition = {1},
   keywords = {metallothioneines; phytochelatins; glutathione; mercury electrode;catalytic reactions; Brdicka reaction;peak H},
   language = {eng},
   location = {Thessaloniki, Greece},
   pages = {431 - 431},
   publisher = {International Society of Electrochemistry},
   title = {Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode},
   year = {2004}
}

TY  - JOUR
ID  - 567329
AU  - Vacek, Jan - Trnková, Libuše - Jelen, František - Kizek, René
PY  - 2004
TI  - Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode
PB  - International Society of Electrochemistry
CY  - Thessaloniki, Greece
KW  - metallothioneines
KW  - phytochelatins
KW  - glutathione
KW  - mercury electrode;catalytic reactions
KW  - Brdicka reaction;peak H
N2  - The group cysteine-containing peptides (metallothioneines, phytochelatins, and glutathione) plays an important role in active homeostasis and detoxification of metals in animal, plant, and/or microorganism cells. In this study we describe a novel approach to electroanalysis of cys-containing peptides and proteins. This approach is based on catalytic processes (presodium wave, peak H, Brdicka current) proceeding at negative potentials (ca. 1.8 V, Ag/AgCl/3M KCl) on the hanging mercury drop electrode (HMDE). A presodium wave is current-potential response accompanied with catalytic hydrogen evolution from aqueous solutions in the presence of proteins. It was found that the sulfhydryl(SH) groups in proteins are responsible for presodium catalytic processes. The highest sensitivity in the determination was observed with derivative chronopotentiometric stripping analysis (CPSA), which monitored the catalytic signal known as peak H. The determination of the low content of Cys-peptides was successfully performed in a cobalt(III)-containing solution with ammonia buffer by differential pulse voltammetry (DPV). The CPSA (peak H) and DPV (Brdicka reaction) can be used in connection with the adsorptive transfer stripping (AdTS) technique, which is capable of determining nano/femtomole levels of metallothioneine, phytochelatin and glutathione in mL of the sample.
ER  -

VACEK, Jan, Libuše TRNKOVÁ, František JELEN a René KIZEK. Electrochemical determination of cysteine-containing biomolecules by means of catalytic reactions on a mercury electrode. In \textit{Book of Abstracts - 55th Annual Meeting of the ISE}. 1. vyd. Thessaloniki, Greece: International Society of Electrochemistry, 2004, s.~431 - 431.

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