Determination of isoflavones using high-performance liquid
chromatography with electrochemical detection (HPLC- ED)

D 2004

Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED)

KLEJDUS, Bořivoj; Jitka PETRLOVÁ; David POTĚŠIL; Libuše TRNKOVÁ; Radka MIKELOVÁ et al.

Základní údaje

Originální název

Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED)

Název česky

Stanovení isoflavonů za použití HPLC s elektrochemickou detekcí

Autoři

KLEJDUS, Bořivoj; Jitka PETRLOVÁ; David POTĚŠIL; Libuše TRNKOVÁ; Radka MIKELOVÁ; Josef ZEHNÁLEK; René KIZEK a Vlastimil KUBÁŇ

Vydání

1. vyd. Thessaloniki, Greece, Book of Abstracts - 55th Annual Meeting of the ISE, od s. 433 - 433, 1 s. 2004

Nakladatel

International Society of Electrochemistry

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/04:00011236

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

HPLC-ED; square wave voltammetry; isoflavones; daidzein; genistein; soybean products

Štítky

daidzein, genistein, HPLC-ED, Isoflavones, soybean products, square wave voltammetry

Změněno: 15. 2. 2005 21:33, prof. RNDr. Libuše Trnková, CSc.

Anotace

ORIG CZ

V originále

A highly sensitive HPLC-ED was developed for the determination of isoflavones. The electrochemical behaviour of daidzein and genistein was studied on a carbon paste electrode by square wave voltammetry. The electrochemical results obtained were used for the development of the HPLC-ED method. The HPLC-ED system used consisted of two solvent delivery pumps and an eight-channel CoulArray electrochemical detector. Isoflavones were separated on an Atlantis dC18 column using a mobile phase consisting of acetonitrile (solvent A) and 0.15 M acetate buffer of pH 5.5 (solvent B) at a flow rate of 0.4 mL/min. The full scan of multi-channel coulometric detection was tested and an optimal potential at 450 mV was chosen for our purposes. The calibration curves were linear (daidzein R2 = 0.9993 and genistein R2 = 0.9987). The relative standard deviation (R.S.D., n = 5) was 2.5 %. The detection limits for daidzein and genistein were 480 and 394 pg/mL (1.8 and 1.5 nM), respectively. Isoflavones extracted from soybean products (farina, meat, milk) by the pressurised solvent extraction procedure and isoflavones contained in human urine were determined by this HPLC-ED method.

Česky

Stanovení isoflavonů za použití HPLC s elektrochemickou detekcí

Návaznosti

GA203/02/0422, projekt VaV

Název: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

Investor: Grantová agentura ČR, Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

IAA1163201, projekt VaV

Název: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Investor: Akademie věd ČR, Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Citovat

KLEJDUS, Bořivoj; Jitka PETRLOVÁ; David POTĚŠIL; Libuše TRNKOVÁ; Radka MIKELOVÁ; Josef ZEHNÁLEK; René KIZEK a Vlastimil KUBÁŇ. Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED). In Book of Abstracts - 55th Annual Meeting of the ISE. 1. vyd. Thessaloniki, Greece: International Society of Electrochemistry, 2004, s. 433 - 433.

@inproceedings{567330,
   author = {Klejdus, Bořivoj and Petrlová, Jitka and Potěšil, David and Trnková, Libuše and Mikelová, Radka and Zehnálek, Josef and Kizek, René and Kubáň, Vlastimil},
   address = {Thessaloniki, Greece},
   booktitle = {Book of Abstracts - 55th Annual Meeting of the ISE},
   edition = {1},
   keywords = {HPLC-ED; square wave voltammetry; isoflavones; daidzein; genistein; soybean products},
   language = {eng},
   location = {Thessaloniki, Greece},
   pages = {433 - 433},
   publisher = {International Society of Electrochemistry},
   title = {Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED)},
   year = {2004}
}

TY  - CONF
ID  - 567330
AU  - Klejdus, Bořivoj - Petrlová, Jitka - Potěšil, David - Trnková, Libuše - Mikelová, Radka - Zehnálek, Josef - Kizek, René - Kubáň, Vlastimil
PY  - 2004
TI  - Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED)
PB  - International Society of Electrochemistry
CY  - Thessaloniki, Greece
KW  - HPLC-ED
KW  - square wave voltammetry
KW  - isoflavones
KW  - daidzein
KW  - genistein
KW  - soybean products
N2  - A highly sensitive HPLC-ED was developed for the determination of isoflavones. The electrochemical behaviour of daidzein and genistein was studied on a carbon paste electrode by square wave voltammetry. The electrochemical results obtained were used for the development of the HPLC-ED method. The HPLC-ED system used consisted of two solvent delivery pumps and an eight-channel CoulArray electrochemical detector. Isoflavones were separated on an Atlantis dC18 column using a mobile phase consisting of acetonitrile (solvent A) and 0.15 M acetate buffer of pH 5.5 (solvent B) at a flow rate of 0.4 mL/min. The full scan of multi-channel coulometric detection was tested and an optimal potential at 450 mV was chosen for our purposes. The calibration curves were linear (daidzein R2 = 0.9993 and genistein R2 = 0.9987). The relative standard deviation (R.S.D., n = 5) was 2.5 %. The detection limits for daidzein and genistein were 480 and 394 pg/mL (1.8 and 1.5 nM), respectively. Isoflavones extracted from soybean products (farina, meat, milk) by the pressurised solvent extraction procedure and isoflavones contained in human urine were determined by this HPLC-ED method.
ER  -

KLEJDUS, Bořivoj; Jitka PETRLOVÁ; David POTĚŠIL; Libuše TRNKOVÁ; Radka MIKELOVÁ; Josef ZEHNÁLEK; René KIZEK a Vlastimil KUBÁŇ. Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED). In \textit{Book of Abstracts - 55th Annual Meeting of the ISE}. 1. vyd. Thessaloniki, Greece: International Society of Electrochemistry, 2004, s.~433 - 433.

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