Analysis of phytochelatin (g-Glu-Cys)2-Gly by a multi-channel
electrochemical detector in online connection with
reversed-phase liquid chromatography

D 2004

Analysis of phytochelatin (g-Glu-Cys)2-Gly by a multi-channel electrochemical detector in online connection with reversed-phase liquid chromatography

PETRLOVÁ, Jitka; David POTĚŠIL; Vojtěch ADAM; Bořivoj KLEJDUS; Libuše TRNKOVÁ et al.

Základní údaje

Originální název

Analysis of phytochelatin (g-Glu-Cys)2-Gly by a multi-channel electrochemical detector in online connection with reversed-phase liquid chromatography

Název česky

Analýza fytochelatinu (g-Glu-Cys)2-Gly pomocí multikanálového elektrochemického detektoru v online napojení na HPLC s reverzní fází

Autoři

PETRLOVÁ, Jitka; David POTĚŠIL; Vojtěch ADAM; Bořivoj KLEJDUS; Libuše TRNKOVÁ; František JELEN; Josef ZEHNÁLEK; Ladislav HAVEL a René KIZEK

Vydání

1. vyd. Thessaloniki, Greece, Book of Abstracts I - 55th Annual Meeting of the ISE, od s. 434 - 434, 1 s. 2004

Nakladatel

International Society of Electrochemistry

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/04:00011237

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Reversed-phase liquid chromatography; electrochemical detector; phytochelatin; limit detection

Štítky

electrochemical detector, limit detection, phytochelatin, Reversed-phase liquid chromatography

Změněno: 22. 5. 2009 21:51, prof. RNDr. Vojtěch Adam, Ph.D.

Anotace

ORIG CZ

V originále

Phytochelatins (PCs) are Cys-rich peptides produced by plant or some animal cells as protective molecules against heavy-metal ions in the cytoplasm. PCs have the basic formula (g-Glu-Cys)n-X (n: 2 to 11, X: terminal amino acid as Gly). PCs contain cysteine SH-groups and can form metal-PC complexes in cell environment. For the analysis of PCs different separation and detection methods were used, including electrochemical methods. In this work we present determination of PC2 using HPLC-ED. For this purpose hydrodynamic voltammetric measurements were used at a potential of 0.7 V giving the best current response. We tested the effect of temperature of the chromatographic column and the electrochemical detector on chromatographic separation. The best results were observed at 25 C. An HPLC-ED system consisted of two solvent delivery pumps and an eight-channel CoulArray electrochemical detector. This detector was located after the HPLC system with an XDB C18 reverse-phase column (mobile phase: trifluoroacetic acid/acetonitrile; flow-rate 0.4 mL/min). It was found that the PC2 limit detection was improved by the addition of ascorbic acid (in millimolar concentration) to the sample. This combination of HPLC with electrochemical detection is a suitable tool for determination of the picomolar content of PC2 on the column.

Česky

Analýza fytochelatinu (g-Glu-Cys)2-Gly pomocí multikanálového elektrochemického detektoru v online napojení na HPLC s reverzní fází

Návaznosti

GA203/02/0422, projekt VaV

Název: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

Investor: Grantová agentura ČR, Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí

IAA1163201, projekt VaV

Název: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Investor: Akademie věd ČR, Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin

Citovat

PETRLOVÁ, Jitka; David POTĚŠIL; Vojtěch ADAM; Bořivoj KLEJDUS; Libuše TRNKOVÁ; František JELEN; Josef ZEHNÁLEK; Ladislav HAVEL a René KIZEK. Analysis of phytochelatin (g-Glu-Cys)2-Gly by a multi-channel electrochemical detector in online connection with reversed-phase liquid chromatography. In Book of Abstracts I - 55th Annual Meeting of the ISE. 1. vyd. Thessaloniki, Greece: International Society of Electrochemistry, 2004, s. 434 - 434.

@inproceedings{567331,
   author = {Petrlová, Jitka and Potěšil, David and Adam, Vojtěch and Klejdus, Bořivoj and Trnková, Libuše and Jelen, František and Zehnálek, Josef and Havel, Ladislav and Kizek, René},
   address = {Thessaloniki, Greece},
   booktitle = {Book of Abstracts I - 55th Annual Meeting of the ISE},
   edition = {1},
   keywords = {Reversed-phase liquid chromatography; electrochemical detector; phytochelatin; limit detection},
   language = {eng},
   location = {Thessaloniki, Greece},
   pages = {434 - 434},
   publisher = {International Society of Electrochemistry},
   title = {Analysis of phytochelatin (g-Glu-Cys)2-Gly by a multi-channel electrochemical detector in online connection with reversed-phase liquid chromatography},
   year = {2004}
}

TY  - CONF
ID  - 567331
AU  - Petrlová, Jitka - Potěšil, David - Adam, Vojtěch - Klejdus, Bořivoj - Trnková, Libuše - Jelen, František - Zehnálek, Josef - Havel, Ladislav - Kizek, René
PY  - 2004
TI  - Analysis of phytochelatin (g-Glu-Cys)2-Gly by a multi-channel electrochemical detector in online connection with reversed-phase liquid chromatography
PB  - International Society of Electrochemistry
CY  - Thessaloniki, Greece
KW  - Reversed-phase liquid chromatography
KW  - electrochemical detector
KW  - phytochelatin
KW  - limit detection
N2  - Phytochelatins (PCs) are Cys-rich peptides produced by plant or some animal cells as protective molecules against heavy-metal ions in the cytoplasm. PCs have the basic formula (g-Glu-Cys)n-X (n: 2 to 11, X: terminal amino acid as Gly). PCs contain cysteine SH-groups and can form metal-PC complexes in cell environment. For the analysis of PCs different separation and detection methods were used, including electrochemical methods. In this work we present determination of PC2 using HPLC-ED. For this purpose hydrodynamic voltammetric measurements were used at a potential of 0.7 V giving the best current response. We tested the effect of temperature of the chromatographic column and the electrochemical detector on chromatographic separation. The best results were observed at 25 C. An HPLC-ED system consisted of two solvent delivery pumps and an eight-channel CoulArray electrochemical detector. This detector was located after the HPLC system with an XDB C18 reverse-phase column (mobile phase: trifluoroacetic acid/acetonitrile; flow-rate 0.4 mL/min). It was found that the PC2 limit detection was improved by the addition of ascorbic acid (in millimolar concentration) to the sample. This combination of HPLC with electrochemical detection is a suitable tool for determination of the picomolar content of PC2 on the column.
ER  -

PETRLOVÁ, Jitka; David POTĚŠIL; Vojtěch ADAM; Bořivoj KLEJDUS; Libuše TRNKOVÁ; František JELEN; Josef ZEHNÁLEK; Ladislav HAVEL a René KIZEK. Analysis of phytochelatin (g-Glu-Cys)2-Gly by a multi-channel electrochemical detector in online connection with reversed-phase liquid chromatography. In \textit{Book of Abstracts I - 55th Annual Meeting of the ISE}. 1. vyd. Thessaloniki, Greece: International Society of Electrochemistry, 2004, s.~434 - 434.

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