Separace mikrocystinů kapilární elektrochromatografií spojenou
s hmotnostní spektrometrií

D 2005

Separace mikrocystinů kapilární elektrochromatografií spojenou s hmotnostní spektrometrií

ZEISBERGEROVÁ, Marta, Vratislav KOŠŤÁL, Josef ČÁSLAVSKÝ, Zdeněk GLATZ, Vladislav KAHLE et. al.

Základní údaje

Originální název

Separace mikrocystinů kapilární elektrochromatografií spojenou s hmotnostní spektrometrií

Název česky

Separace mikrocystinů kapilární elektrochromatografií spojenou s hmotnostní spektrometrií

Autoři

ZEISBERGEROVÁ, Marta (203 Česká republika, garant), Vratislav KOŠŤÁL (203 Česká republika), Josef ČÁSLAVSKÝ (203 Česká republika), Zdeněk GLATZ (203 Česká republika) a Vladislav KAHLE (203 Česká republika)

Vydání

1. vyd. Olomouc, Sborník konference "Chiranal 2005" s. 100-100, 2005

Nakladatel

K. analytické chemie př.f. UP

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/05:00012392

Organizační jednotka

Přírodovědecká fakulta

ISBN

80-244-0984-4

Klíčová slova anglicky

microcystines; CEC; MS

Štítky

CEC, microcystines, MS

Změněno: 19. 5. 2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.

Anotace

ORIG CZ

V originále

Enzymes are biological catalysts that play an important role in biochemical reactions necessary for normal growth, maturation and reproduction through whole live world. Due to their low concentrations in the samples containing large amount of other proteins, direct measurements of enzymes by masses are impossible. However enzymes can be measured more easily by their catalytic activities, which are the most relevant properties of enzymes in the biochemical context. The accurate measurement of enzymatic activity in biological samples is important in many fields of biochemistry, not only in routine biochemistry and in fundamental research but also in clinical and pharmacological research and diagnosis. The enzyme assay is also important procedure in elucidation of enzyme properties and function. Determination of kinetic parameters is usually undertaken to characterize an enzyme, to provide a quantitative evaluation of substrate specificity and to study kinetic mechanisms. In the last decade capillary electromigration separation techniques have become powerful techniques, which can provide highly efficient separations and large peak capacities. Measurements of enzyme activity by CE, which have been under active investigation in recent years, include the enzyme reaction prior CE analysis and the enzyme reaction in the separation capillaries during CE also known as electrophoretically mediated microanalysis (EMMA). Both these approaches were applied in the complex study on rhodanese, important enzyme catalysing detoxication of cyanide to less toxic thiocyanate after reaction with a sulfur donor, such as thiosulfate. The enzyme activity of rhodanese and the effects of temperature and pH on enzymatic reaction were evaluated by the off-capillary approach, whereas the kinetic parameters such as the Michaelis and inhibition constants were determined by means of the EMMA methodology. The applications of this new methodology on other enzyme systems will be also shortly given.

Česky

V přednášce byly shrnuty výsledky dosažené při studiu enzymů pomoci CE v off-line a on-line uspořádání.

Návaznosti

GA203/02/1447, projekt VaV

Název: Kapilární elektrochromatografie v lineárních a planárních mikrostrukturách s využitím polymerních náplní

Investor: Grantová agentura ČR, Kapilární elektrochromatografie v lineárních a planárních mikrostrukturách s využitím polymerních náplní

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

ZEISBERGEROVÁ, Marta, Vratislav KOŠŤÁL, Josef ČÁSLAVSKÝ, Zdeněk GLATZ a Vladislav KAHLE. Separace mikrocystinů kapilární elektrochromatografií spojenou s hmotnostní spektrometrií. In Sborník konference "Chiranal 2005". 1. vyd. Olomouc: K. analytické chemie př.f. UP, 2005, s. 100. ISBN 80-244-0984-4.

@inproceedings{568367,
   author = {Zeisbergerová, Marta and Košťál, Vratislav and Čáslavský, Josef and Glatz, Zdeněk and Kahle, Vladislav},
   address = {Olomouc},
   booktitle = {Sborník konference "Chiranal 2005"},
   edition = {1},
   keywords = {microcystines; CEC; MS},
   language = {eng},
   location = {Olomouc},
   isbn = {80-244-0984-4},
   pages = {100-100},
   publisher = {K. analytické chemie př.f. UP},
   title = {Separace mikrocystinů kapilární elektrochromatografií spojenou s hmotnostní spektrometrií},
   year = {2005}
}

TY  - JOUR
ID  - 568367
AU  - Zeisbergerová, Marta - Košťál, Vratislav - Čáslavský, Josef - Glatz, Zdeněk - Kahle, Vladislav
PY  - 2005
TI  - Separace mikrocystinů kapilární elektrochromatografií spojenou s hmotnostní spektrometrií
PB  - K. analytické chemie př.f. UP
CY  - Olomouc
SN  - 8024409844
KW  - microcystines
KW  - CEC
KW  - MS
N2  - Enzymes are biological catalysts that play an important role in biochemical reactions necessary for normal growth, maturation and reproduction through whole live world. Due to their low concentrations in the samples containing large amount of other proteins, direct measurements of enzymes by masses are impossible. However enzymes can be measured more easily by their catalytic activities, which are the most relevant properties of enzymes in the biochemical context. The accurate measurement of enzymatic activity in biological samples is important in many fields of biochemistry, not only in routine biochemistry and in fundamental research but also in clinical and pharmacological research and diagnosis. The enzyme assay is also important procedure in elucidation of enzyme properties and function. Determination of kinetic parameters is usually undertaken to characterize an enzyme, to provide a quantitative evaluation of substrate specificity and to study kinetic mechanisms. In the last decade capillary electromigration separation techniques have become powerful techniques, which can provide highly efficient separations and large peak capacities. Measurements of enzyme activity by CE, which have been under active investigation in recent years, include the enzyme reaction prior CE analysis and the enzyme reaction in the separation capillaries during CE also known as electrophoretically mediated microanalysis (EMMA). Both these approaches were applied in the complex study on rhodanese, important enzyme catalysing detoxication of cyanide to less toxic thiocyanate after reaction with a sulfur donor, such as thiosulfate. The enzyme activity of rhodanese and the effects of temperature and pH on enzymatic reaction were evaluated by the off-capillary approach, whereas the kinetic parameters such as the Michaelis and inhibition constants were determined by means of the EMMA methodology. The applications of this new methodology on other enzyme systems will be also shortly given.
ER  -

ZEISBERGEROVÁ, Marta, Vratislav KOŠŤÁL, Josef ČÁSLAVSKÝ, Zdeněk GLATZ a Vladislav KAHLE. Separace mikrocystinů kapilární elektrochromatografií spojenou s hmotnostní spektrometrií. In \textit{Sborník konference ''Chiranal 2005''}. 1. vyd. Olomouc: K. analytické chemie př.f. UP, 2005, s.~100. ISBN~80-244-0984-4.

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