Use of immobilized cytochrome c as a ligand for affinity
chromatography of thiosulfate dehydrogenase from
Acidithiobacillus ferrooxidans

J 2005

Use of immobilized cytochrome c as a ligand for affinity chromatography of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans

JANICZEK, Oldřich; Blanka POKORNÁ; Jana ZEMANOVÁ a Martin MANDL

Základní údaje

Originální název

Use of immobilized cytochrome c as a ligand for affinity chromatography of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans

Název česky

Využití imobilizovaného cytochromu c při purifikaci thiosulfátdehydrogenasy u bakterie Acidithiobacillus ferrooxidans

Autoři

JANICZEK, Oldřich; Blanka POKORNÁ; Jana ZEMANOVÁ a Martin MANDL

Vydání

Journal of Biotechnology, Amsterdam, Elsevier, 2005, 0168-1656

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.687

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/05:00012444

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000229342100010

Klíčová slova anglicky

Affinity chromatography; Acidithiobacillus; thiosulfate dehydrogenase; immobilized cytochrome c

Štítky

Acidithiobacillus, affinity chromatography, immobilized cytochrome c, thiosulfate dehydrogenase

Změněno: 2. 5. 2005 09:11, doc. RNDr. Oldřich Janiczek, CSc.

Anotace

ORIG CZ

V originále

Three matrixes were used for immobilizing the cytochrome c: Sepharose CL-4B, Silasorb SPH amine and a laboratory-prepared new matrix based on crosslinked triazine (2,4,6-tris (aminoethylamine)-1,3,5-triazine) (TAT). Cytochrome c was immobilized on the matrixes by several procedures and the amount of incorporated cytochrome c was determined. Cytochrome c immobilized on Sepharose CL-4B with periodate activation, cytochrome c immobilized on Silasorbamine with carbodiimide activation and cytochrome c immobilized on crosslinked triazine were suitable for purification of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans. The yield with all matrixes was about 90%. The purification factor of the above matrixes was about 15. A new matrix based on TAT with cytochrome c represented a suitable way for thiosulfate dehydrogenase purification.

Česky

Využití imobilizovaného cytochromu c při purifikaci thiosulfátdehydrogenasy u bakterie Acidithiobacillus ferrooxidans

Návaznosti

GA525/04/1309, projekt VaV

Název: Bakteriální oxidace anorganických sirných látek, ekologické aspekty

Investor: Grantová agentura ČR, Bakteriální oxidace anorganických sirných látek, ekologické aspekty

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

JANICZEK, Oldřich; Blanka POKORNÁ; Jana ZEMANOVÁ a Martin MANDL. Use of immobilized cytochrome c as a ligand for affinity chromatography of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans. Journal of Biotechnology. Amsterdam: Elsevier, 2005, roč. 117, č. 1, s. 293-298. ISSN 0168-1656.

@article{570573,
   author = {Janiczek, Oldřich and Pokorná, Blanka and Zemanová, Jana and Mandl, Martin},
   article_location = {Amsterdam},
   article_number = {1},
   keywords = {Affinity chromatography; Acidithiobacillus; thiosulfate dehydrogenase; immobilized cytochrome c},
   language = {eng},
   issn = {0168-1656},
   journal = {Journal of Biotechnology},
   title = {Use of immobilized cytochrome c as a ligand for affinity chromatography of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans},
   volume = {117},
   year = {2005}
}

TY  - JOUR
ID  - 570573
AU  - Janiczek, Oldřich - Pokorná, Blanka - Zemanová, Jana - Mandl, Martin
PY  - 2005
TI  - Use of immobilized cytochrome c as a ligand for affinity chromatography of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans
JF  - Journal of Biotechnology
VL  - 117
IS  - 1
SP  - 293-298
EP  - 293-298
PB  - Elsevier
SN  - 01681656
KW  - Affinity chromatography
KW  - Acidithiobacillus
KW  - thiosulfate dehydrogenase
KW  - immobilized cytochrome c
N2  - Three matrixes were used for immobilizing the cytochrome c: Sepharose CL-4B, Silasorb SPH amine and a laboratory-prepared new matrix based on crosslinked triazine (2,4,6-tris (aminoethylamine)-1,3,5-triazine) (TAT). Cytochrome c was immobilized on the matrixes by several procedures and the amount of incorporated cytochrome c was determined. Cytochrome c immobilized on Sepharose CL-4B with periodate activation, cytochrome c immobilized on Silasorbamine with carbodiimide activation and cytochrome c immobilized on crosslinked triazine were suitable for purification of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans. The yield with all matrixes was about 90%. The purification factor of the above matrixes was about 15. A new matrix based on TAT with cytochrome c represented a suitable way for thiosulfate dehydrogenase purification.
ER  -

JANICZEK, Oldřich; Blanka POKORNÁ; Jana ZEMANOVÁ a Martin MANDL. Use of immobilized cytochrome c as a ligand for affinity chromatography of thiosulfate dehydrogenase from Acidithiobacillus ferrooxidans. \textit{Journal of Biotechnology}. Amsterdam: Elsevier, 2005, roč.~117, č.~1, s.~293-298. ISSN~0168-1656.

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